Identification of inhibitors of ovarian cancer stem-like cells by high-throughput screening

<p>Abstract</p> <p>Background</p> <p>Ovarian cancer stem cells are characterized by self-renewal capacity, ability to differentiate into distinct lineages, as well as higher invasiveness and resistance to many anticancer agents. Since they may be responsible for the rec...

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Main Authors: Mezencev Roman, Wang Lijuan, McDonald John F
Format: Article
Language:English
Published: BMC 2012-10-01
Series:Journal of Ovarian Research
Subjects:
Online Access:http://www.ovarianresearch.com/content/5/1/30
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spelling doaj-b08e009205884115a48e0cacb022b8d52020-11-25T02:35:46ZengBMCJournal of Ovarian Research1757-22152012-10-01513010.1186/1757-2215-5-30Identification of inhibitors of ovarian cancer stem-like cells by high-throughput screeningMezencev RomanWang LijuanMcDonald John F<p>Abstract</p> <p>Background</p> <p>Ovarian cancer stem cells are characterized by self-renewal capacity, ability to differentiate into distinct lineages, as well as higher invasiveness and resistance to many anticancer agents. Since they may be responsible for the recurrence of ovarian cancer after initial response to chemotherapy, development of new therapies targeting this special cellular subpopulation embedded within bulk ovarian cancers is warranted.</p> <p>Methods</p> <p>A high-throughput screening (HTS) campaign was performed with 825 compounds from the Mechanistic Set chemical library [Developmental Therapeutics Program (DTP)/National Cancer Institute (NCI)] against ovarian cancer stem-like cells (CSC) using a resazurin-based cell cytotoxicity assay. Identified sets of active compounds were projected onto self-organizing maps to identify their putative cellular response groups.</p> <p>Results</p> <p>From 793 screening compounds with evaluable data, 158 were found to have significant inhibitory effects on ovarian CSC. Computational analysis indicates that the majority of these compounds are associated with mitotic cellular responses.</p> <p>Conclusions</p> <p>Our HTS has uncovered a number of candidate compounds that may, after further testing, prove effective in targeting both ovarian CSC and their more differentiated progeny.</p> http://www.ovarianresearch.com/content/5/1/30High-throughput screeningOvarian cancerCancer stem cells
collection DOAJ
language English
format Article
sources DOAJ
author Mezencev Roman
Wang Lijuan
McDonald John F
spellingShingle Mezencev Roman
Wang Lijuan
McDonald John F
Identification of inhibitors of ovarian cancer stem-like cells by high-throughput screening
Journal of Ovarian Research
High-throughput screening
Ovarian cancer
Cancer stem cells
author_facet Mezencev Roman
Wang Lijuan
McDonald John F
author_sort Mezencev Roman
title Identification of inhibitors of ovarian cancer stem-like cells by high-throughput screening
title_short Identification of inhibitors of ovarian cancer stem-like cells by high-throughput screening
title_full Identification of inhibitors of ovarian cancer stem-like cells by high-throughput screening
title_fullStr Identification of inhibitors of ovarian cancer stem-like cells by high-throughput screening
title_full_unstemmed Identification of inhibitors of ovarian cancer stem-like cells by high-throughput screening
title_sort identification of inhibitors of ovarian cancer stem-like cells by high-throughput screening
publisher BMC
series Journal of Ovarian Research
issn 1757-2215
publishDate 2012-10-01
description <p>Abstract</p> <p>Background</p> <p>Ovarian cancer stem cells are characterized by self-renewal capacity, ability to differentiate into distinct lineages, as well as higher invasiveness and resistance to many anticancer agents. Since they may be responsible for the recurrence of ovarian cancer after initial response to chemotherapy, development of new therapies targeting this special cellular subpopulation embedded within bulk ovarian cancers is warranted.</p> <p>Methods</p> <p>A high-throughput screening (HTS) campaign was performed with 825 compounds from the Mechanistic Set chemical library [Developmental Therapeutics Program (DTP)/National Cancer Institute (NCI)] against ovarian cancer stem-like cells (CSC) using a resazurin-based cell cytotoxicity assay. Identified sets of active compounds were projected onto self-organizing maps to identify their putative cellular response groups.</p> <p>Results</p> <p>From 793 screening compounds with evaluable data, 158 were found to have significant inhibitory effects on ovarian CSC. Computational analysis indicates that the majority of these compounds are associated with mitotic cellular responses.</p> <p>Conclusions</p> <p>Our HTS has uncovered a number of candidate compounds that may, after further testing, prove effective in targeting both ovarian CSC and their more differentiated progeny.</p>
topic High-throughput screening
Ovarian cancer
Cancer stem cells
url http://www.ovarianresearch.com/content/5/1/30
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