Convergent antibody evolution and clonotype expansion following influenza virus vaccination.
Recent advances in high-throughput single cell sequencing have opened up new avenues into the investigation of B cell receptor (BCR) repertoires. In this study, PBMCs were collected from 17 human participants vaccinated with the split-inactivated influenza virus vaccine during the 2016-2017 influenz...
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doaj-b09e8c0027f148049f12503edc5197fb2021-08-17T04:31:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01162e024725310.1371/journal.pone.0247253Convergent antibody evolution and clonotype expansion following influenza virus vaccination.David ForgacsRodrigo B AbreuGiuseppe A SauttoGreg A KirchenbaumElliott DrabekKevin S WilliamsonDongkyoon KimDaniel E EmerlingTed M RossRecent advances in high-throughput single cell sequencing have opened up new avenues into the investigation of B cell receptor (BCR) repertoires. In this study, PBMCs were collected from 17 human participants vaccinated with the split-inactivated influenza virus vaccine during the 2016-2017 influenza season. A combination of Immune Repertoire Capture (IRCTM) technology and IgG sequencing was performed on ~7,800 plasmablast (PB) cells and preferential IgG heavy-light chain pairings were investigated. In some participants, a single expanded clonotype accounted for ~22% of their PB BCR repertoire. Approximately 60% (10/17) of participants experienced convergent evolution, possessing public PBs that were elicited independently in multiple participants. Binding profiles of one private and three public PBs confirmed they were all subtype-specific, cross-reactive hemagglutinin (HA) head-directed antibodies. Collectively, this high-resolution antibody repertoire analysis demonstrated the impact evolution can have on BCRs in response to influenza virus vaccination, which can guide future universal influenza prophylactic approaches.https://doi.org/10.1371/journal.pone.0247253 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
David Forgacs Rodrigo B Abreu Giuseppe A Sautto Greg A Kirchenbaum Elliott Drabek Kevin S Williamson Dongkyoon Kim Daniel E Emerling Ted M Ross |
spellingShingle |
David Forgacs Rodrigo B Abreu Giuseppe A Sautto Greg A Kirchenbaum Elliott Drabek Kevin S Williamson Dongkyoon Kim Daniel E Emerling Ted M Ross Convergent antibody evolution and clonotype expansion following influenza virus vaccination. PLoS ONE |
author_facet |
David Forgacs Rodrigo B Abreu Giuseppe A Sautto Greg A Kirchenbaum Elliott Drabek Kevin S Williamson Dongkyoon Kim Daniel E Emerling Ted M Ross |
author_sort |
David Forgacs |
title |
Convergent antibody evolution and clonotype expansion following influenza virus vaccination. |
title_short |
Convergent antibody evolution and clonotype expansion following influenza virus vaccination. |
title_full |
Convergent antibody evolution and clonotype expansion following influenza virus vaccination. |
title_fullStr |
Convergent antibody evolution and clonotype expansion following influenza virus vaccination. |
title_full_unstemmed |
Convergent antibody evolution and clonotype expansion following influenza virus vaccination. |
title_sort |
convergent antibody evolution and clonotype expansion following influenza virus vaccination. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2021-01-01 |
description |
Recent advances in high-throughput single cell sequencing have opened up new avenues into the investigation of B cell receptor (BCR) repertoires. In this study, PBMCs were collected from 17 human participants vaccinated with the split-inactivated influenza virus vaccine during the 2016-2017 influenza season. A combination of Immune Repertoire Capture (IRCTM) technology and IgG sequencing was performed on ~7,800 plasmablast (PB) cells and preferential IgG heavy-light chain pairings were investigated. In some participants, a single expanded clonotype accounted for ~22% of their PB BCR repertoire. Approximately 60% (10/17) of participants experienced convergent evolution, possessing public PBs that were elicited independently in multiple participants. Binding profiles of one private and three public PBs confirmed they were all subtype-specific, cross-reactive hemagglutinin (HA) head-directed antibodies. Collectively, this high-resolution antibody repertoire analysis demonstrated the impact evolution can have on BCRs in response to influenza virus vaccination, which can guide future universal influenza prophylactic approaches. |
url |
https://doi.org/10.1371/journal.pone.0247253 |
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