Morphological and Molecular Features of Porcine Mesenchymal Stem Cells Derived From Different Types of Synovial Membrane, and Genetic Background of Cell Donors
Synovial mesenchymal stem cells (SMSCs) have become a great cell source for musculoskeletal stem cell research, especially related to cartilage and bone tissue regeneration, due to their superior cell proliferation properties and multidifferentiation potential into various cell lineages. This study...
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doaj-b0a029463d4847bf83a20954a9c0c6e22020-12-09T04:41:51ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2020-12-01810.3389/fcell.2020.601212601212Morphological and Molecular Features of Porcine Mesenchymal Stem Cells Derived From Different Types of Synovial Membrane, and Genetic Background of Cell DonorsPuntita Siengdee0Michael Oster1Henry Reyer2Torsten Viergutz3Klaus Wimmers4Siriluck Ponsuksili5Institute for Genome Biology, Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, GermanyInstitute for Genome Biology, Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, GermanyInstitute for Genome Biology, Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, GermanyInstitute for Reproductive Biology, Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, GermanyInstitute for Genome Biology, Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, GermanyInstitute for Genome Biology, Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, GermanySynovial mesenchymal stem cells (SMSCs) have become a great cell source for musculoskeletal stem cell research, especially related to cartilage and bone tissue regeneration, due to their superior cell proliferation properties and multidifferentiation potential into various cell lineages. This study revealed isolation methods, culture conditions, and morphological and molecular characterization of SMSCs derived fibrous synovium (FS) and adipose synovium (FP) of two pig breeds differing in growth performance [German Landrace (DL), and fat deposition (Angeln Saddleback (AS)]. Herein, FS possessed nucleated cell numbers nearly twice as high as those of FP at Passage 0. SMSCs derived from different types of synovial membrane and genetic background show similar cell morphologies and immunophenotypes, which were assessed by cell surface epitopes and multilineage differentiation potential, but differ significantly in their molecular characteristics. In addition, transcripts of SMSCs from AS were more enriched in IGF-1 signaling and VEGF ligand receptor, while SMSCs from DL were more enriched in growth hormone signaling and bone metabolism. The results indicate that genetics and tissues play significant roles for SMSC characteristics so that SMSCs can be traced back to the original cell donor and be used for fine turning in applications of medical research and therapies.https://www.frontiersin.org/articles/10.3389/fcell.2020.601212/fullmesenchymal stem cellsSMSCssynovial membraneporcine synoviumGerman LandraceAngeln Saddleback |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Puntita Siengdee Michael Oster Henry Reyer Torsten Viergutz Klaus Wimmers Siriluck Ponsuksili |
spellingShingle |
Puntita Siengdee Michael Oster Henry Reyer Torsten Viergutz Klaus Wimmers Siriluck Ponsuksili Morphological and Molecular Features of Porcine Mesenchymal Stem Cells Derived From Different Types of Synovial Membrane, and Genetic Background of Cell Donors Frontiers in Cell and Developmental Biology mesenchymal stem cells SMSCs synovial membrane porcine synovium German Landrace Angeln Saddleback |
author_facet |
Puntita Siengdee Michael Oster Henry Reyer Torsten Viergutz Klaus Wimmers Siriluck Ponsuksili |
author_sort |
Puntita Siengdee |
title |
Morphological and Molecular Features of Porcine Mesenchymal Stem Cells Derived From Different Types of Synovial Membrane, and Genetic Background of Cell Donors |
title_short |
Morphological and Molecular Features of Porcine Mesenchymal Stem Cells Derived From Different Types of Synovial Membrane, and Genetic Background of Cell Donors |
title_full |
Morphological and Molecular Features of Porcine Mesenchymal Stem Cells Derived From Different Types of Synovial Membrane, and Genetic Background of Cell Donors |
title_fullStr |
Morphological and Molecular Features of Porcine Mesenchymal Stem Cells Derived From Different Types of Synovial Membrane, and Genetic Background of Cell Donors |
title_full_unstemmed |
Morphological and Molecular Features of Porcine Mesenchymal Stem Cells Derived From Different Types of Synovial Membrane, and Genetic Background of Cell Donors |
title_sort |
morphological and molecular features of porcine mesenchymal stem cells derived from different types of synovial membrane, and genetic background of cell donors |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Cell and Developmental Biology |
issn |
2296-634X |
publishDate |
2020-12-01 |
description |
Synovial mesenchymal stem cells (SMSCs) have become a great cell source for musculoskeletal stem cell research, especially related to cartilage and bone tissue regeneration, due to their superior cell proliferation properties and multidifferentiation potential into various cell lineages. This study revealed isolation methods, culture conditions, and morphological and molecular characterization of SMSCs derived fibrous synovium (FS) and adipose synovium (FP) of two pig breeds differing in growth performance [German Landrace (DL), and fat deposition (Angeln Saddleback (AS)]. Herein, FS possessed nucleated cell numbers nearly twice as high as those of FP at Passage 0. SMSCs derived from different types of synovial membrane and genetic background show similar cell morphologies and immunophenotypes, which were assessed by cell surface epitopes and multilineage differentiation potential, but differ significantly in their molecular characteristics. In addition, transcripts of SMSCs from AS were more enriched in IGF-1 signaling and VEGF ligand receptor, while SMSCs from DL were more enriched in growth hormone signaling and bone metabolism. The results indicate that genetics and tissues play significant roles for SMSC characteristics so that SMSCs can be traced back to the original cell donor and be used for fine turning in applications of medical research and therapies. |
topic |
mesenchymal stem cells SMSCs synovial membrane porcine synovium German Landrace Angeln Saddleback |
url |
https://www.frontiersin.org/articles/10.3389/fcell.2020.601212/full |
work_keys_str_mv |
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