A Novel One-Step Fabricated, Droplet-Based Electrochemical Sensor for Facile Biochemical Assays
A simple, novel concept for the one-step fabrication of a low-cost, easy-to-use droplet-based electrochemical (EC) sensor is described, in which the EC reagents are contained in a droplet and the droplet assay is operated on a simple planar surface instead of in a complicated closed channel/chamber....
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doaj-b0cc5f605f864632b1c59e4a75d8940c2020-11-24T21:10:33ZengMDPI AGSensors1424-82202016-08-01168123110.3390/s16081231s16081231A Novel One-Step Fabricated, Droplet-Based Electrochemical Sensor for Facile Biochemical AssaysYong Yao0Chunsun Zhang1MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, ChinaMOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, ChinaA simple, novel concept for the one-step fabrication of a low-cost, easy-to-use droplet-based electrochemical (EC) sensor is described, in which the EC reagents are contained in a droplet and the droplet assay is operated on a simple planar surface instead of in a complicated closed channel/chamber. In combination with an elegant carbon electrode configuration, screen-printed on a widely available polyethylene terephthalate (PET) substrate, the developed sensor exhibits a stable solution-restriction capacity and acceptable EC response, and thus can be used directly for the detection of different analytes (including ascorbic acid (AA), copper ions (Cu2+), 2′-deoxyguanosine 5′-triphosphate (dGTP) and ferulic acid (FA)), without any pretreatment. The obtained, acceptable linear ranges/detection limits for AA, Cu2+, dGTP and FA are 0.5–10/0.415 mM, (0.0157–0.1574 and 0.1574–1.5736)/0.011 mM, 0.01–0.1/0.008 mM and 0.0257–0.515/0.024 mM, respectively. Finally, the utility of the droplet-based EC sensor was demonstrated for the determination of AA in two commercial beverages, and of Cu2+ in two water samples, with reliable recovery and good stability. The applicability of the droplet-based sensor demonstrates that the proposed EC strategy is potentially a cost-effective solution for a series of biochemical sensing applications in public health, environmental monitoring, and the developing world.http://www.mdpi.com/1424-8220/16/8/1231one-step fabricationdroplet-based sensorelectrochemical sensingbiochemical assays |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yong Yao Chunsun Zhang |
spellingShingle |
Yong Yao Chunsun Zhang A Novel One-Step Fabricated, Droplet-Based Electrochemical Sensor for Facile Biochemical Assays Sensors one-step fabrication droplet-based sensor electrochemical sensing biochemical assays |
author_facet |
Yong Yao Chunsun Zhang |
author_sort |
Yong Yao |
title |
A Novel One-Step Fabricated, Droplet-Based Electrochemical Sensor for Facile Biochemical Assays |
title_short |
A Novel One-Step Fabricated, Droplet-Based Electrochemical Sensor for Facile Biochemical Assays |
title_full |
A Novel One-Step Fabricated, Droplet-Based Electrochemical Sensor for Facile Biochemical Assays |
title_fullStr |
A Novel One-Step Fabricated, Droplet-Based Electrochemical Sensor for Facile Biochemical Assays |
title_full_unstemmed |
A Novel One-Step Fabricated, Droplet-Based Electrochemical Sensor for Facile Biochemical Assays |
title_sort |
novel one-step fabricated, droplet-based electrochemical sensor for facile biochemical assays |
publisher |
MDPI AG |
series |
Sensors |
issn |
1424-8220 |
publishDate |
2016-08-01 |
description |
A simple, novel concept for the one-step fabrication of a low-cost, easy-to-use droplet-based electrochemical (EC) sensor is described, in which the EC reagents are contained in a droplet and the droplet assay is operated on a simple planar surface instead of in a complicated closed channel/chamber. In combination with an elegant carbon electrode configuration, screen-printed on a widely available polyethylene terephthalate (PET) substrate, the developed sensor exhibits a stable solution-restriction capacity and acceptable EC response, and thus can be used directly for the detection of different analytes (including ascorbic acid (AA), copper ions (Cu2+), 2′-deoxyguanosine 5′-triphosphate (dGTP) and ferulic acid (FA)), without any pretreatment. The obtained, acceptable linear ranges/detection limits for AA, Cu2+, dGTP and FA are 0.5–10/0.415 mM, (0.0157–0.1574 and 0.1574–1.5736)/0.011 mM, 0.01–0.1/0.008 mM and 0.0257–0.515/0.024 mM, respectively. Finally, the utility of the droplet-based EC sensor was demonstrated for the determination of AA in two commercial beverages, and of Cu2+ in two water samples, with reliable recovery and good stability. The applicability of the droplet-based sensor demonstrates that the proposed EC strategy is potentially a cost-effective solution for a series of biochemical sensing applications in public health, environmental monitoring, and the developing world. |
topic |
one-step fabrication droplet-based sensor electrochemical sensing biochemical assays |
url |
http://www.mdpi.com/1424-8220/16/8/1231 |
work_keys_str_mv |
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1716756071262978048 |