Development of a fluorescent immunoassay strip for the rapid quantitative detection of cadmium in rice

• Main Authors: Yumei Xing, Xiaoling Wu, Liqiang Liu, Jianping Zhu, Liguang Xu, Hua Kuang
• Format: Article
• Language: English
• Published: Taylor & Francis Group 2020-01-01
• Series: Food and Agricultural Immunology
• Subjects: cadmium; rice samples; monoclonal antibody; fluorescent immunoassay test strip
• Online Access: http://dx.doi.org/10.1080/09540105.2020.1741518

In this study, we produced a monoclonal antibody (mAb) 2F7 against cadmium (Cd) with IC50 of 0.20 ng/mL. The cross-reactivity of mAb 2F7 with other eight metals was < 1%. A fluorescent immunoassay strip was developed for the determination of Cd in rice. The cut-off value by visual inspection of the test strip was 50 μg/kg, and the quantitative detection range determined by the strip reader was 3.86–48.92 μg/kg. Both ic-ELISA and the fluorescent immunoassay strip can be used to detect Cd rice. Therefore, our developed fluorescent immunoassay strip may be an effective tool for detecting Cd in rice.