Effects of repeated application of sulfadiazine-contaminated pig manure on the abundance and diversity of ammonia and nitrite oxidizers in the root-rhizosphere complex of pasture plants under field conditions
In a field experiment, the impact of repeated application of the antibiotic sulfadiazine (SDZ)-contaminated pig manure was assessed on functional microbial communities involved in ammonia and nitrite oxidation in the root-rhizosphere complexes (RRCs) of diverse plants composing a pasture. We surveye...
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doaj-b0ddedce01924acc8c253dc9d9fbdd552020-11-24T22:31:30ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2013-02-01410.3389/fmicb.2013.0002240252Effects of repeated application of sulfadiazine-contaminated pig manure on the abundance and diversity of ammonia and nitrite oxidizers in the root-rhizosphere complex of pasture plants under field conditionsJulien eOllivier0Julien eOllivier1Daniela eSchacht2Reimo eKindler3Joost eGroeneweg4Marion eEngel5Berndt-Michael eWilke6Kristina eKleineidam7Michael eSchloter8Technical University MunichHelmholtz Zentrum MünchenHelmholtz Zentrum MünchenTechnical University BerlinForschungszentrum JülichHelmholtz Zentrum MünchenTechnical University BerlinHelmholtz Zentrum MünchenHelmholtz Zentrum MünchenIn a field experiment, the impact of repeated application of the antibiotic sulfadiazine (SDZ)-contaminated pig manure was assessed on functional microbial communities involved in ammonia and nitrite oxidation in the root-rhizosphere complexes (RRCs) of diverse plants composing a pasture. We surveyed the abundance of ammonia-oxidizing archaea (AOA) and bacteria (AOB) as well as Nitrobacter- and Nitrospira-like nitrite-oxidizing bacteria (NOB) by quantitative PCR, and the diversity of amoA AOA and Nitrobacter-like nxrA amplicons using a cloning-sequencing approach. Whereas the first SDZ-contaminated manure application caused only slight effects on the investigated microbial communities and did not change the diversity and abundance pattern significantly, the second application of SDZ-contaminated manure induced an up to 15-fold increased ratio of AOA:AOB and a reduction of nrxA genes. The diversity of AOA amoA increased after the second application of SDZ-contaminated manure compared to the control treatment whereas a clear reduction of nrxA OTUs was visible in the same samples. The results indicate that the application of SDZ may principally affect nitrite oxidation by NOB and alternative pathways like nitrite reduction might be favored under these conditions.http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00022/fullNitrificationSulfadiazinerhizosphereammonia-oxidizing microbesnitrite-oxidizing bacteria |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Julien eOllivier Julien eOllivier Daniela eSchacht Reimo eKindler Joost eGroeneweg Marion eEngel Berndt-Michael eWilke Kristina eKleineidam Michael eSchloter |
spellingShingle |
Julien eOllivier Julien eOllivier Daniela eSchacht Reimo eKindler Joost eGroeneweg Marion eEngel Berndt-Michael eWilke Kristina eKleineidam Michael eSchloter Effects of repeated application of sulfadiazine-contaminated pig manure on the abundance and diversity of ammonia and nitrite oxidizers in the root-rhizosphere complex of pasture plants under field conditions Frontiers in Microbiology Nitrification Sulfadiazine rhizosphere ammonia-oxidizing microbes nitrite-oxidizing bacteria |
author_facet |
Julien eOllivier Julien eOllivier Daniela eSchacht Reimo eKindler Joost eGroeneweg Marion eEngel Berndt-Michael eWilke Kristina eKleineidam Michael eSchloter |
author_sort |
Julien eOllivier |
title |
Effects of repeated application of sulfadiazine-contaminated pig manure on the abundance and diversity of ammonia and nitrite oxidizers in the root-rhizosphere complex of pasture plants under field conditions |
title_short |
Effects of repeated application of sulfadiazine-contaminated pig manure on the abundance and diversity of ammonia and nitrite oxidizers in the root-rhizosphere complex of pasture plants under field conditions |
title_full |
Effects of repeated application of sulfadiazine-contaminated pig manure on the abundance and diversity of ammonia and nitrite oxidizers in the root-rhizosphere complex of pasture plants under field conditions |
title_fullStr |
Effects of repeated application of sulfadiazine-contaminated pig manure on the abundance and diversity of ammonia and nitrite oxidizers in the root-rhizosphere complex of pasture plants under field conditions |
title_full_unstemmed |
Effects of repeated application of sulfadiazine-contaminated pig manure on the abundance and diversity of ammonia and nitrite oxidizers in the root-rhizosphere complex of pasture plants under field conditions |
title_sort |
effects of repeated application of sulfadiazine-contaminated pig manure on the abundance and diversity of ammonia and nitrite oxidizers in the root-rhizosphere complex of pasture plants under field conditions |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2013-02-01 |
description |
In a field experiment, the impact of repeated application of the antibiotic sulfadiazine (SDZ)-contaminated pig manure was assessed on functional microbial communities involved in ammonia and nitrite oxidation in the root-rhizosphere complexes (RRCs) of diverse plants composing a pasture. We surveyed the abundance of ammonia-oxidizing archaea (AOA) and bacteria (AOB) as well as Nitrobacter- and Nitrospira-like nitrite-oxidizing bacteria (NOB) by quantitative PCR, and the diversity of amoA AOA and Nitrobacter-like nxrA amplicons using a cloning-sequencing approach. Whereas the first SDZ-contaminated manure application caused only slight effects on the investigated microbial communities and did not change the diversity and abundance pattern significantly, the second application of SDZ-contaminated manure induced an up to 15-fold increased ratio of AOA:AOB and a reduction of nrxA genes. The diversity of AOA amoA increased after the second application of SDZ-contaminated manure compared to the control treatment whereas a clear reduction of nrxA OTUs was visible in the same samples. The results indicate that the application of SDZ may principally affect nitrite oxidation by NOB and alternative pathways like nitrite reduction might be favored under these conditions. |
topic |
Nitrification Sulfadiazine rhizosphere ammonia-oxidizing microbes nitrite-oxidizing bacteria |
url |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00022/full |
work_keys_str_mv |
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