Translational control of enzyme scavenger expression with toxin-induced micro RNA switches
Abstract Biological computation requires in vivo control of molecular behavior to progress development of autonomous devices. miRNA switches represent excellent, easily engineerable synthetic biology tools to achieve user-defined gene regulation. Here we present the construction of a synthetic netwo...
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2021-01-01
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Online Access: | https://doi.org/10.1038/s41598-021-81679-6 |
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doaj-b0e97f0d97544fadb1b5f8b8a0c9ba752021-01-31T16:24:48ZengNature Publishing GroupScientific Reports2045-23222021-01-0111111210.1038/s41598-021-81679-6Translational control of enzyme scavenger expression with toxin-induced micro RNA switchesNina M. Pollak0Justin J. Cooper-White1Joanne Macdonald2Genecology Research Centre, University of the Sunshine CoastAustralian Institute for Bioengineering and Nanotechnology, The University of QueenslandGenecology Research Centre, University of the Sunshine CoastAbstract Biological computation requires in vivo control of molecular behavior to progress development of autonomous devices. miRNA switches represent excellent, easily engineerable synthetic biology tools to achieve user-defined gene regulation. Here we present the construction of a synthetic network to implement detoxification functionality. We employed a modular design strategy by engineering toxin-induced control of an enzyme scavenger. Our miRNA switch results show moderate synthetic expression control over a biologically active detoxification enzyme molecule, using an established design protocol. However, following a new design approach, we demonstrated an evolutionarily designed miRNA switch to more effectively activate enzyme activity than synthetically designed versions, allowing markedly improved extrinsic user-defined control with a toxin as inducer. Our straightforward new design approach is simple to implement and uses easily accessible web-based databases and prediction tools. The ability to exert control of toxicity demonstrates potential for modular detoxification systems that provide a pathway to new therapeutic and biocomputing applications.https://doi.org/10.1038/s41598-021-81679-6 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nina M. Pollak Justin J. Cooper-White Joanne Macdonald |
spellingShingle |
Nina M. Pollak Justin J. Cooper-White Joanne Macdonald Translational control of enzyme scavenger expression with toxin-induced micro RNA switches Scientific Reports |
author_facet |
Nina M. Pollak Justin J. Cooper-White Joanne Macdonald |
author_sort |
Nina M. Pollak |
title |
Translational control of enzyme scavenger expression with toxin-induced micro RNA switches |
title_short |
Translational control of enzyme scavenger expression with toxin-induced micro RNA switches |
title_full |
Translational control of enzyme scavenger expression with toxin-induced micro RNA switches |
title_fullStr |
Translational control of enzyme scavenger expression with toxin-induced micro RNA switches |
title_full_unstemmed |
Translational control of enzyme scavenger expression with toxin-induced micro RNA switches |
title_sort |
translational control of enzyme scavenger expression with toxin-induced micro rna switches |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-01-01 |
description |
Abstract Biological computation requires in vivo control of molecular behavior to progress development of autonomous devices. miRNA switches represent excellent, easily engineerable synthetic biology tools to achieve user-defined gene regulation. Here we present the construction of a synthetic network to implement detoxification functionality. We employed a modular design strategy by engineering toxin-induced control of an enzyme scavenger. Our miRNA switch results show moderate synthetic expression control over a biologically active detoxification enzyme molecule, using an established design protocol. However, following a new design approach, we demonstrated an evolutionarily designed miRNA switch to more effectively activate enzyme activity than synthetically designed versions, allowing markedly improved extrinsic user-defined control with a toxin as inducer. Our straightforward new design approach is simple to implement and uses easily accessible web-based databases and prediction tools. The ability to exert control of toxicity demonstrates potential for modular detoxification systems that provide a pathway to new therapeutic and biocomputing applications. |
url |
https://doi.org/10.1038/s41598-021-81679-6 |
work_keys_str_mv |
AT ninampollak translationalcontrolofenzymescavengerexpressionwithtoxininducedmicrornaswitches AT justinjcooperwhite translationalcontrolofenzymescavengerexpressionwithtoxininducedmicrornaswitches AT joannemacdonald translationalcontrolofenzymescavengerexpressionwithtoxininducedmicrornaswitches |
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