Characterization of the in vitro response to NaCI on callus in multiplication and regeneration phase of some rice genotypes

• Main Authors: Pedro Orellana Pérez, Pablo Leandro Peña González
• Format: Article
• Language: English
• Published: Universidad Central Marta Abreu de Las Villas 2003-01-01
• Series: Biotecnología Vegetal
• Online Access: https://revista.ibp.co.cu/index.php/BV/article/view/217

This paper present the in vitro response in two different development stages of four rice somaclons for the salt tolerance using five NaCl concentrations. The evaluated somaclons were: ‘IAC 25’, ‘IAC 26’, ‘R 13 - K1 - 1- 2’ and ‘R 20 - K1 -1 – 2’. The varieties ‘Pokkali’ and ‘Mi 48’, were used as resistant and susceptible controls, respectively. It was find a differential reaction for tolerants and susceptibles genotypes for the salt tolerance in dependence of the NaCl concentration in the callus growing and regeneration stages. The regeneration stage showed higher sensibility to NaCl concentration. According with these results, the somaclon ‘IAC 25’ like the ‘Pokkali’ variety, expressed salt tolerance until 1.5% of NaCl in both development stages. The others somaclons presented low salt tolerance levels compared with the resistant variety. In the multiplication stage of the callus two continuous subcultures on the culture media with NaCl are necessary in order to express a clear differential reaction between tolerant and susceptible genotypes to this sal. Key words: callus, levels, regeneration, salinity, somaclons