Identification and Immunogenicity of African Swine Fever Virus Antigens
African swine fever (ASF) is a lethal haemorrhagic disease of domestic pigs for which there is no vaccine. Strains of the virus with reduced virulence can provide protection against related virulent strains of ASFV, but protection is not 100% and there are concerns about the safety profile of such v...
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doaj-b10c042a55054a12bf5f5e5e02d4fe092020-11-25T02:42:11ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-06-011010.3389/fimmu.2019.01318433949Identification and Immunogenicity of African Swine Fever Virus AntigensChristopher L. Netherton0Lynnette C. Goatley1Ana Luisa Reis2Raquel Portugal3Rachel H. Nash4Sophie B. Morgan5Lynden Gault6Raquel Nieto7Veronica Norlin8Carmina Gallardo9Chak-Sum Ho10Pedro J. Sánchez-Cordón11Geraldine Taylor12Linda K. Dixon13The Pirbright Institute, Woking, United KingdomThe Pirbright Institute, Woking, United KingdomThe Pirbright Institute, Woking, United KingdomThe Pirbright Institute, Woking, United KingdomThe Pirbright Institute, Woking, United KingdomThe Pirbright Institute, Woking, United KingdomGift of Life Michigan Histocompatibility Laboratory, Ann Arbor, MI, United StatesEuropean Union Reference Laboratory for ASF, Centro de Investigación en Sanidad Animal-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid, SpainGift of Life Michigan Histocompatibility Laboratory, Ann Arbor, MI, United StatesEuropean Union Reference Laboratory for ASF, Centro de Investigación en Sanidad Animal-Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid, SpainGift of Life Michigan Histocompatibility Laboratory, Ann Arbor, MI, United StatesThe Pirbright Institute, Woking, United KingdomThe Pirbright Institute, Woking, United KingdomThe Pirbright Institute, Woking, United KingdomAfrican swine fever (ASF) is a lethal haemorrhagic disease of domestic pigs for which there is no vaccine. Strains of the virus with reduced virulence can provide protection against related virulent strains of ASFV, but protection is not 100% and there are concerns about the safety profile of such viruses. However, they provide a useful tool for understanding the immune response to ASFV and previous studies using the low virulent isolate OUR T88/3 have shown that CD8+ cells are crucial for protection. In order to develop a vaccine that stimulates an effective anti-ASFV T-cell response we need to know which of the >150 viral proteins are recognized by the cellular immune response. Therefore, we used a gamma interferon ELIspot assay to screen for viral proteins recognized by lymphocytes from ASF-immune pigs using peptides corresponding to 133 proteins predicted to be encoded by OUR T88/3. Eighteen antigens that were recognized by ASFV-specific lymphocytes were then incorporated into adenovirus and MVA vectors, which were used in immunization and challenge experiments in pigs. We present a systematic characterization of the cellular immune response to this devastating disease and identify proteins capable of inducing ASFV-specific cellular and humoral immune responses in pigs. Pools of viral vectors expressing these genes did not protect animals from severe disease, but did reduce viremia in a proportion of pigs following ASFV challenge.https://www.frontiersin.org/article/10.3389/fimmu.2019.01318/fullT-cellantigen presentationswineAfrican swine fever (virus)disease enhancementELISPOT assay for interferon gamma |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Christopher L. Netherton Lynnette C. Goatley Ana Luisa Reis Raquel Portugal Rachel H. Nash Sophie B. Morgan Lynden Gault Raquel Nieto Veronica Norlin Carmina Gallardo Chak-Sum Ho Pedro J. Sánchez-Cordón Geraldine Taylor Linda K. Dixon |
spellingShingle |
Christopher L. Netherton Lynnette C. Goatley Ana Luisa Reis Raquel Portugal Rachel H. Nash Sophie B. Morgan Lynden Gault Raquel Nieto Veronica Norlin Carmina Gallardo Chak-Sum Ho Pedro J. Sánchez-Cordón Geraldine Taylor Linda K. Dixon Identification and Immunogenicity of African Swine Fever Virus Antigens Frontiers in Immunology T-cell antigen presentation swine African swine fever (virus) disease enhancement ELISPOT assay for interferon gamma |
author_facet |
Christopher L. Netherton Lynnette C. Goatley Ana Luisa Reis Raquel Portugal Rachel H. Nash Sophie B. Morgan Lynden Gault Raquel Nieto Veronica Norlin Carmina Gallardo Chak-Sum Ho Pedro J. Sánchez-Cordón Geraldine Taylor Linda K. Dixon |
author_sort |
Christopher L. Netherton |
title |
Identification and Immunogenicity of African Swine Fever Virus Antigens |
title_short |
Identification and Immunogenicity of African Swine Fever Virus Antigens |
title_full |
Identification and Immunogenicity of African Swine Fever Virus Antigens |
title_fullStr |
Identification and Immunogenicity of African Swine Fever Virus Antigens |
title_full_unstemmed |
Identification and Immunogenicity of African Swine Fever Virus Antigens |
title_sort |
identification and immunogenicity of african swine fever virus antigens |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2019-06-01 |
description |
African swine fever (ASF) is a lethal haemorrhagic disease of domestic pigs for which there is no vaccine. Strains of the virus with reduced virulence can provide protection against related virulent strains of ASFV, but protection is not 100% and there are concerns about the safety profile of such viruses. However, they provide a useful tool for understanding the immune response to ASFV and previous studies using the low virulent isolate OUR T88/3 have shown that CD8+ cells are crucial for protection. In order to develop a vaccine that stimulates an effective anti-ASFV T-cell response we need to know which of the >150 viral proteins are recognized by the cellular immune response. Therefore, we used a gamma interferon ELIspot assay to screen for viral proteins recognized by lymphocytes from ASF-immune pigs using peptides corresponding to 133 proteins predicted to be encoded by OUR T88/3. Eighteen antigens that were recognized by ASFV-specific lymphocytes were then incorporated into adenovirus and MVA vectors, which were used in immunization and challenge experiments in pigs. We present a systematic characterization of the cellular immune response to this devastating disease and identify proteins capable of inducing ASFV-specific cellular and humoral immune responses in pigs. Pools of viral vectors expressing these genes did not protect animals from severe disease, but did reduce viremia in a proportion of pigs following ASFV challenge. |
topic |
T-cell antigen presentation swine African swine fever (virus) disease enhancement ELISPOT assay for interferon gamma |
url |
https://www.frontiersin.org/article/10.3389/fimmu.2019.01318/full |
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