Detection of pathological alpha-synuclein aggregates in human iPSC-derived neurons and tissue

• Main Authors: Iva Stojkovska, Joseph R. Mazzulli
• Format: Article
• Language: English
• Published: Elsevier 2021-03-01
• Series: STAR Protocols
• Subjects: Molecular biology; Neuroscience; Protein biochemistry
• Online Access: http://www.sciencedirect.com/science/article/pii/S2666166721000794

Summary: The accumulation of proteins into insoluble aggregates is a common feature of several neurodegenerative diseases. Aggregated α-synuclein is a major component of Lewy bodies that pathologically define Parkinson's disease (PD). Here, we present methods for the detection of pathogenic conformations of α-synuclein in induced pluripotent stem cell (iPSC) patient-derived neuron models and brain tissue. These methods can be applied to studies of PD pathogenesis and the discovery of novel therapeutics that restore physiological α-synuclein.For complete details on the use and execution of this protocol, please refer to Cuddy et al. (2019) and Zunke et al. (2018).