Quantification of polyphenolic compounds and relative gene expression studies of phenylpropanoid pathway in apple (Malus domestica Borkh) in response to Venturia inaequalis infection

Quantification of polyphenolic compounds and relative gene expression studies of phenylpropanoid pathway in apple (Malus domestica Borkh) in response to Venturia inaequalis infection

Apple (Malus domestica Borkh) is rich in phenolic compounds, which may enhance resistance to scab disease caused by Venturia inaequalis. In present study, apple cv. Golden Delicious was used for estimation of total phenols, flavonoids and quantification of six individual phenolic compounds. between...

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Main Authors: Sheikh Mansoor, Vikas Sharma, Mudasir A. Mir, Javid I. Mir, Sajad un Nabi, Nazeer Ahmed, Jawaher Alkahtani, Mona S. Alwahibi, Khalid Z. Masoodi
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:Saudi Journal of Biological Sciences
Subjects:
Apple
Venturia inaequalis
Polyphenols
Phenylalanine ammonia lyase
Resistance
Online Access:http://www.sciencedirect.com/science/article/pii/S1319562X20304137
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spelling doaj-b1170b63f4f844f5afbcd4dc526fdc862020-12-03T04:30:25ZengElsevierSaudi Journal of Biological Sciences1319-562X2020-12-01271233973404Quantification of polyphenolic compounds and relative gene expression studies of phenylpropanoid pathway in apple (Malus domestica Borkh) in response to Venturia inaequalis infectionSheikh Mansoor0Vikas Sharma1Mudasir A. Mir2Javid I. Mir3Sajad un Nabi4Nazeer Ahmed5Jawaher Alkahtani6Mona S. Alwahibi7Khalid Z. Masoodi8Division of Biochemistry, Faculty of Basic Sciences, SKUAST, Jammu 180009, IndiaDivision of Biochemistry, Faculty of Basic Sciences, SKUAST, Jammu 180009, IndiaDivision of Plant Biotechnology, SKUAST, Shalimar, Kashmir 190025, IndiaICAR – Cental Institute of Temperate Horticulture, Rangreth, Srinagar, IndiaICAR – Cental Institute of Temperate Horticulture, Rangreth, Srinagar, IndiaDivision of Plant Biotechnology, SKUAST, Shalimar, Kashmir 190025, IndiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi ArabiaDivision of Plant Biotechnology, SKUAST, Shalimar, Kashmir 190025, India; Corresponding author.Apple (Malus domestica Borkh) is rich in phenolic compounds, which may enhance resistance to scab disease caused by Venturia inaequalis. In present study, apple cv. Golden Delicious was used for estimation of total phenols, flavonoids and quantification of six individual phenolic compounds. between control vs inoculated samples at different inoculation stages. The relative gene expression of phenylalanine ammonia lyase, chalcone synthase and flavanone 3 hydroxylase increased and polyphenolic compounds were constitutively upregulated at different post-inoculation stages. Data suggest that synthesis and accumulation of polyphenols is closely related with disease resistance against Venturia inaequalis. This study may play a vital role in understanding and finding out the governing mechanisms of scab resistance.http://www.sciencedirect.com/science/article/pii/S1319562X20304137AppleVenturia inaequalisPolyphenolsPhenylalanine ammonia lyaseResistance
collection DOAJ
language English
format Article
sources DOAJ
author Sheikh Mansoor
Vikas Sharma
Mudasir A. Mir
Javid I. Mir
Sajad un Nabi
Nazeer Ahmed
Jawaher Alkahtani
Mona S. Alwahibi
Khalid Z. Masoodi
spellingShingle Sheikh Mansoor
Vikas Sharma
Mudasir A. Mir
Javid I. Mir
Sajad un Nabi
Nazeer Ahmed
Jawaher Alkahtani
Mona S. Alwahibi
Khalid Z. Masoodi
Quantification of polyphenolic compounds and relative gene expression studies of phenylpropanoid pathway in apple (Malus domestica Borkh) in response to Venturia inaequalis infection
Saudi Journal of Biological Sciences
Apple
Venturia inaequalis
Polyphenols
Phenylalanine ammonia lyase
Resistance
author_facet Sheikh Mansoor
Vikas Sharma
Mudasir A. Mir
Javid I. Mir
Sajad un Nabi
Nazeer Ahmed
Jawaher Alkahtani
Mona S. Alwahibi
Khalid Z. Masoodi
author_sort Sheikh Mansoor
title Quantification of polyphenolic compounds and relative gene expression studies of phenylpropanoid pathway in apple (Malus domestica Borkh) in response to Venturia inaequalis infection
title_short Quantification of polyphenolic compounds and relative gene expression studies of phenylpropanoid pathway in apple (Malus domestica Borkh) in response to Venturia inaequalis infection
title_full Quantification of polyphenolic compounds and relative gene expression studies of phenylpropanoid pathway in apple (Malus domestica Borkh) in response to Venturia inaequalis infection
title_fullStr Quantification of polyphenolic compounds and relative gene expression studies of phenylpropanoid pathway in apple (Malus domestica Borkh) in response to Venturia inaequalis infection
title_full_unstemmed Quantification of polyphenolic compounds and relative gene expression studies of phenylpropanoid pathway in apple (Malus domestica Borkh) in response to Venturia inaequalis infection
title_sort quantification of polyphenolic compounds and relative gene expression studies of phenylpropanoid pathway in apple (malus domestica borkh) in response to venturia inaequalis infection
publisher Elsevier
series Saudi Journal of Biological Sciences
issn 1319-562X
publishDate 2020-12-01
description Apple (Malus domestica Borkh) is rich in phenolic compounds, which may enhance resistance to scab disease caused by Venturia inaequalis. In present study, apple cv. Golden Delicious was used for estimation of total phenols, flavonoids and quantification of six individual phenolic compounds. between control vs inoculated samples at different inoculation stages. The relative gene expression of phenylalanine ammonia lyase, chalcone synthase and flavanone 3 hydroxylase increased and polyphenolic compounds were constitutively upregulated at different post-inoculation stages. Data suggest that synthesis and accumulation of polyphenols is closely related with disease resistance against Venturia inaequalis. This study may play a vital role in understanding and finding out the governing mechanisms of scab resistance.
topic Apple
Venturia inaequalis
Polyphenols
Phenylalanine ammonia lyase
Resistance
url http://www.sciencedirect.com/science/article/pii/S1319562X20304137
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