Potential role for the tumor suppressor CYLD in brain and notochord development
Abstract Background The cylindromatosis (CYLD) tumor suppressor is a microtubule‐associated deubiquitinase that plays a critical role in the regulation of cell signaling and contributes to a variety of physiological and pathological processes. However, the functions of CYLD in zebrafish are less wel...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Wiley
2021-06-01
|
Series: | Thoracic Cancer |
Subjects: | |
Online Access: | https://doi.org/10.1111/1759-7714.13973 |
id |
doaj-b12788e18d7248bba75c9ac9d6eaf4e1 |
---|---|
record_format |
Article |
spelling |
doaj-b12788e18d7248bba75c9ac9d6eaf4e12021-06-14T09:46:00ZengWileyThoracic Cancer1759-77061759-77142021-06-0112121900190810.1111/1759-7714.13973Potential role for the tumor suppressor CYLD in brain and notochord developmentTe Li0Yiyan Wang1Dengwen Li2Jun Zhou3Bo Zhang4Xianfei He5State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences Nankai University Tianjin ChinaKey Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, Peking University Genome Editing Research Center, College of Life Sciences Peking University Beijing ChinaState Key Laboratory of Medicinal Chemical Biology, College of Life Sciences Nankai University Tianjin ChinaState Key Laboratory of Medicinal Chemical Biology, College of Life Sciences Nankai University Tianjin ChinaKey Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, Peking University Genome Editing Research Center, College of Life Sciences Peking University Beijing ChinaState Key Laboratory of Medicinal Chemical Biology, College of Life Sciences Nankai University Tianjin ChinaAbstract Background The cylindromatosis (CYLD) tumor suppressor is a microtubule‐associated deubiquitinase that plays a critical role in the regulation of cell signaling and contributes to a variety of physiological and pathological processes. However, the functions of CYLD in zebrafish are less well known, particularly with regard to their development and physiology. In this context, we investigated the loss of function of CYLD in zebrafish via transcription activator‐like effector nuclease (TALEN)‐based gene deletion. Methods Semi‐quantitative RT‐PCR was used to quantify CYLD mRNA expression in zebrafish embryos at various developmental stages. We also performed whole‐mount in situ hybridization to further assess the dynamic expression and distribution of CYLD in the entire zebrafish embryos at different stages. In addition, we deleted CYLD in zebrafish with TALENs to investigate its potential impact on embryonic development. Results The expression of CYLD mRNA varied during early embryonic development. The CYLD mRNA localized to the brain and notochord of developing zebrafish embryos. Homozygous deletion of CYLD resulted in embryonic death before 8 h post‐fertilization. Conclusions CYLD appears to play an important role in central nervous system development in zebrafish. Although severe embryonic death restricted analysis of homozygous mutants, further research into the role of CYLD in central nervous system development is warranted.https://doi.org/10.1111/1759-7714.13973cylindromatosistumor suppressorCYLDTALENbrain |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Te Li Yiyan Wang Dengwen Li Jun Zhou Bo Zhang Xianfei He |
spellingShingle |
Te Li Yiyan Wang Dengwen Li Jun Zhou Bo Zhang Xianfei He Potential role for the tumor suppressor CYLD in brain and notochord development Thoracic Cancer cylindromatosis tumor suppressor CYLD TALEN brain |
author_facet |
Te Li Yiyan Wang Dengwen Li Jun Zhou Bo Zhang Xianfei He |
author_sort |
Te Li |
title |
Potential role for the tumor suppressor CYLD in brain and notochord development |
title_short |
Potential role for the tumor suppressor CYLD in brain and notochord development |
title_full |
Potential role for the tumor suppressor CYLD in brain and notochord development |
title_fullStr |
Potential role for the tumor suppressor CYLD in brain and notochord development |
title_full_unstemmed |
Potential role for the tumor suppressor CYLD in brain and notochord development |
title_sort |
potential role for the tumor suppressor cyld in brain and notochord development |
publisher |
Wiley |
series |
Thoracic Cancer |
issn |
1759-7706 1759-7714 |
publishDate |
2021-06-01 |
description |
Abstract Background The cylindromatosis (CYLD) tumor suppressor is a microtubule‐associated deubiquitinase that plays a critical role in the regulation of cell signaling and contributes to a variety of physiological and pathological processes. However, the functions of CYLD in zebrafish are less well known, particularly with regard to their development and physiology. In this context, we investigated the loss of function of CYLD in zebrafish via transcription activator‐like effector nuclease (TALEN)‐based gene deletion. Methods Semi‐quantitative RT‐PCR was used to quantify CYLD mRNA expression in zebrafish embryos at various developmental stages. We also performed whole‐mount in situ hybridization to further assess the dynamic expression and distribution of CYLD in the entire zebrafish embryos at different stages. In addition, we deleted CYLD in zebrafish with TALENs to investigate its potential impact on embryonic development. Results The expression of CYLD mRNA varied during early embryonic development. The CYLD mRNA localized to the brain and notochord of developing zebrafish embryos. Homozygous deletion of CYLD resulted in embryonic death before 8 h post‐fertilization. Conclusions CYLD appears to play an important role in central nervous system development in zebrafish. Although severe embryonic death restricted analysis of homozygous mutants, further research into the role of CYLD in central nervous system development is warranted. |
topic |
cylindromatosis tumor suppressor CYLD TALEN brain |
url |
https://doi.org/10.1111/1759-7714.13973 |
work_keys_str_mv |
AT teli potentialroleforthetumorsuppressorcyldinbrainandnotochorddevelopment AT yiyanwang potentialroleforthetumorsuppressorcyldinbrainandnotochorddevelopment AT dengwenli potentialroleforthetumorsuppressorcyldinbrainandnotochorddevelopment AT junzhou potentialroleforthetumorsuppressorcyldinbrainandnotochorddevelopment AT bozhang potentialroleforthetumorsuppressorcyldinbrainandnotochorddevelopment AT xianfeihe potentialroleforthetumorsuppressorcyldinbrainandnotochorddevelopment |
_version_ |
1721378503036764160 |