Human Organ-Specific Endothelial Cell Heterogeneity

Summary: The endothelium first forms in the blood islands in the extra-embryonic yolk sac and then throughout the embryo to establish circulatory networks that further acquire organ-specific properties during development to support diverse organ functions. Here, we investigated the properties of end...

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Main Authors:	Raluca Marcu, Yoon Jung Choi, Jun Xue, Chelsea L. Fortin, Yuliang Wang, Ryan J. Nagao, Jin Xu, James W. MacDonald, Theo K. Bammler, Charles E. Murry, Kimberly Muczynski, Kelly R. Stevens, Jonathan Himmelfarb, Stephen M. Schwartz, Ying Zheng
Format:	Article
Language:	English
Published:	Elsevier 2018-06-01
Series:	iScience
Online Access:	http://www.sciencedirect.com/science/article/pii/S2589004218300580

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spelling	doaj-b130251fe7784ebc9bfd6b260827d2e02020-11-25T01:37:09ZengElsevieriScience2589-00422018-06-0142035Human Organ-Specific Endothelial Cell HeterogeneityRaluca Marcu0Yoon Jung Choi1Jun Xue2Chelsea L. Fortin3Yuliang Wang4Ryan J. Nagao5Jin Xu6James W. MacDonald7Theo K. Bammler8Charles E. Murry9Kimberly Muczynski10Kelly R. Stevens11Jonathan Himmelfarb12Stephen M. Schwartz13Ying Zheng14Department of Bioengineering, University of Washington, Seattle, WA, USADepartment of Bioengineering, University of Washington, Seattle, WA, USADepartment of Bioengineering, University of Washington, Seattle, WA, USADepartment of Pathology, University of Washington, Seattle, WA, USADepartment of Computer Science & Engineering, University of Washington, Seattle, WA, USA; Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA, USADepartment of Bioengineering, University of Washington, Seattle, WA, USADepartment of Bioengineering, University of Washington, Seattle, WA, USAEnvironmental and Occupational Health Sciences, University of Washington, Seattle, WA, USAEnvironmental and Occupational Health Sciences, University of Washington, Seattle, WA, USADepartment of Bioengineering, University of Washington, Seattle, WA, USA; Department of Pathology, University of Washington, Seattle, WA, USA; Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA, USA; Department of Medicine, University of Washington, Seattle, WA, USADepartment of Medicine, University of Washington, Seattle, WA, USADepartment of Bioengineering, University of Washington, Seattle, WA, USA; Department of Pathology, University of Washington, Seattle, WA, USA; Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA, USADepartment of Medicine, University of Washington, Seattle, WA, USA; Kidney Research Institute, University of Washington, Seattle, WA, USADepartment of Pathology, University of Washington, Seattle, WA, USADepartment of Bioengineering, University of Washington, Seattle, WA, USA; Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA, USA; Kidney Research Institute, University of Washington, Seattle, WA, USA; Corresponding authorSummary: The endothelium first forms in the blood islands in the extra-embryonic yolk sac and then throughout the embryo to establish circulatory networks that further acquire organ-specific properties during development to support diverse organ functions. Here, we investigated the properties of endothelial cells (ECs), isolated from four human major organs—the heart, lung, liver, and kidneys—in individual fetal tissues at three months' gestation, at gene expression, and at cellular function levels. We showed that organ-specific ECs have distinct expression patterns of gene clusters, which support their specific organ development and functions. These ECs displayed distinct barrier properties, angiogenic potential, and metabolic rate and support specific organ functions. Our findings showed the link between human EC heterogeneity and organ development and can be exploited therapeutically to contribute in organ regeneration, disease modeling, as well as guiding differentiation of tissue-specific ECs from human pluripotent stem cells. : Stem Cells Research; Developmental Biology; Biology of Human Development Subject Areas: Stem Cells Research, Developmental Biology, Biology of Human Developmenthttp://www.sciencedirect.com/science/article/pii/S2589004218300580
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Raluca Marcu Yoon Jung Choi Jun Xue Chelsea L. Fortin Yuliang Wang Ryan J. Nagao Jin Xu James W. MacDonald Theo K. Bammler Charles E. Murry Kimberly Muczynski Kelly R. Stevens Jonathan Himmelfarb Stephen M. Schwartz Ying Zheng
spellingShingle	Raluca Marcu Yoon Jung Choi Jun Xue Chelsea L. Fortin Yuliang Wang Ryan J. Nagao Jin Xu James W. MacDonald Theo K. Bammler Charles E. Murry Kimberly Muczynski Kelly R. Stevens Jonathan Himmelfarb Stephen M. Schwartz Ying Zheng Human Organ-Specific Endothelial Cell Heterogeneity iScience
author_facet	Raluca Marcu Yoon Jung Choi Jun Xue Chelsea L. Fortin Yuliang Wang Ryan J. Nagao Jin Xu James W. MacDonald Theo K. Bammler Charles E. Murry Kimberly Muczynski Kelly R. Stevens Jonathan Himmelfarb Stephen M. Schwartz Ying Zheng
author_sort	Raluca Marcu
title	Human Organ-Specific Endothelial Cell Heterogeneity
title_short	Human Organ-Specific Endothelial Cell Heterogeneity
title_full	Human Organ-Specific Endothelial Cell Heterogeneity
title_fullStr	Human Organ-Specific Endothelial Cell Heterogeneity
title_full_unstemmed	Human Organ-Specific Endothelial Cell Heterogeneity
title_sort	human organ-specific endothelial cell heterogeneity
publisher	Elsevier
series	iScience
issn	2589-0042
publishDate	2018-06-01
description	Summary: The endothelium first forms in the blood islands in the extra-embryonic yolk sac and then throughout the embryo to establish circulatory networks that further acquire organ-specific properties during development to support diverse organ functions. Here, we investigated the properties of endothelial cells (ECs), isolated from four human major organs—the heart, lung, liver, and kidneys—in individual fetal tissues at three months' gestation, at gene expression, and at cellular function levels. We showed that organ-specific ECs have distinct expression patterns of gene clusters, which support their specific organ development and functions. These ECs displayed distinct barrier properties, angiogenic potential, and metabolic rate and support specific organ functions. Our findings showed the link between human EC heterogeneity and organ development and can be exploited therapeutically to contribute in organ regeneration, disease modeling, as well as guiding differentiation of tissue-specific ECs from human pluripotent stem cells. : Stem Cells Research; Developmental Biology; Biology of Human Development Subject Areas: Stem Cells Research, Developmental Biology, Biology of Human Development
url	http://www.sciencedirect.com/science/article/pii/S2589004218300580
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Human Organ-Specific Endothelial Cell Heterogeneity

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