Insight into multidrug-resistant Beijing genotype Mycobacterium tuberculosis isolates in Myanmar
Objectives: Myanmar is a World Health Organization high tuberculosis (TB) burden country with a high multidrug-resistant (MDR)-TB burden. Of significance, a high prevalence of the Beijing genotype of Mycobacterium tuberculosis (MTB) among MDR-MTB has been reported previously. A detailed genetic char...
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doaj-b13b02f077714b7cbe8188d8dca4b5bb2020-11-25T00:44:16ZengElsevierInternational Journal of Infectious Diseases1201-97122018-11-0176109119Insight into multidrug-resistant Beijing genotype Mycobacterium tuberculosis isolates in MyanmarLai Lai Sun0Khin Saw Aye1Thida Oo Nan Aye2Mu Mu Shwe3Yukari Fukushima4Stephen V. Gordon5Yasuhiko Suzuki6Chie Nakajima7Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan; Department of Medical Research, Ministry of Health and Sports, Yangon, MyanmarDepartment of Medical Research, Ministry of Health and Sports, Yangon, MyanmarHokkaido University Research Center for Zoonosis Control, Sapporo, Japan; Department of Medical Research, Ministry of Health and Sports, Yangon, MyanmarDepartment of Medical Research, Ministry of Health and Sports, Yangon, MyanmarHokkaido University Research Center for Zoonosis Control, Sapporo, JapanSchool of Veterinary Medicine, University College Dublin, Belfield, Dublin, Ireland; Hokkaido University, GI-CoRE Global Station for Zoonosis Control, Sapporo, JapanHokkaido University Research Center for Zoonosis Control, Sapporo, Japan; Hokkaido University, GI-CoRE Global Station for Zoonosis Control, Sapporo, JapanHokkaido University Research Center for Zoonosis Control, Sapporo, Japan; Hokkaido University, GI-CoRE Global Station for Zoonosis Control, Sapporo, Japan; Corresponding author at: Division of Bioresources, Hokkaido University Research Center for Zoonosis Control, Kita 20, Nishi 10, Kita-ku, Sapporo, 001-0020, Japan.Objectives: Myanmar is a World Health Organization high tuberculosis (TB) burden country with a high multidrug-resistant (MDR)-TB burden. Of significance, a high prevalence of the Beijing genotype of Mycobacterium tuberculosis (MTB) among MDR-MTB has been reported previously. A detailed genetic characterization of TB clinical isolates was performed in order to explore whether there is an association between the prevalence of the Beijing MTB genotype and MDR-TB in Myanmar. Methods: A total of 265 MDR-MTB clinical isolates collected in 2010 and 2012 were subjected to spoligotyping, mycobacterial interspersed repetitive unit–variable number tandem repeat (MIRU-VNTR) analysis, single nucleotide polymorphism (SNP) typing, and drug resistance-associated gene sequencing, including rpoC to detect potential compensatory evolution. Results: Of the total MDR-MTB isolates, 79.2% (210/265) were of the Beijing genotype, the majority of which were the ‘modern’ subtype. Beijing genotype isolates were differentiated by 15-locus MIRU-VNTR and a high clustering rate (53.0%) was observed in the modern subtype. These MIRU-VNTR patterns were similar to Beijing genotype clones spreading across Russia and Central Asia. A high prevalence of katG Ser315Thr, and genetic evidence of extensive drug resistance (XDR) and pre-XDR and compensatory mutations in rpoC were observed among clustered isolates. Conclusions: MDR-MTB strains of the Beijing genotype might be spreading in Myanmar and present a major challenge to TB control in this country. Keywords: Mycobacterium tuberculosis, Multidrug-resistant TB, Beijing genotype, MIRU-VNTR, Myanmarhttp://www.sciencedirect.com/science/article/pii/S1201971218344448 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lai Lai Sun Khin Saw Aye Thida Oo Nan Aye Mu Mu Shwe Yukari Fukushima Stephen V. Gordon Yasuhiko Suzuki Chie Nakajima |
spellingShingle |
Lai Lai Sun Khin Saw Aye Thida Oo Nan Aye Mu Mu Shwe Yukari Fukushima Stephen V. Gordon Yasuhiko Suzuki Chie Nakajima Insight into multidrug-resistant Beijing genotype Mycobacterium tuberculosis isolates in Myanmar International Journal of Infectious Diseases |
author_facet |
Lai Lai Sun Khin Saw Aye Thida Oo Nan Aye Mu Mu Shwe Yukari Fukushima Stephen V. Gordon Yasuhiko Suzuki Chie Nakajima |
author_sort |
Lai Lai Sun |
title |
Insight into multidrug-resistant Beijing genotype Mycobacterium tuberculosis isolates in Myanmar |
title_short |
Insight into multidrug-resistant Beijing genotype Mycobacterium tuberculosis isolates in Myanmar |
title_full |
Insight into multidrug-resistant Beijing genotype Mycobacterium tuberculosis isolates in Myanmar |
title_fullStr |
Insight into multidrug-resistant Beijing genotype Mycobacterium tuberculosis isolates in Myanmar |
title_full_unstemmed |
Insight into multidrug-resistant Beijing genotype Mycobacterium tuberculosis isolates in Myanmar |
title_sort |
insight into multidrug-resistant beijing genotype mycobacterium tuberculosis isolates in myanmar |
publisher |
Elsevier |
series |
International Journal of Infectious Diseases |
issn |
1201-9712 |
publishDate |
2018-11-01 |
description |
Objectives: Myanmar is a World Health Organization high tuberculosis (TB) burden country with a high multidrug-resistant (MDR)-TB burden. Of significance, a high prevalence of the Beijing genotype of Mycobacterium tuberculosis (MTB) among MDR-MTB has been reported previously. A detailed genetic characterization of TB clinical isolates was performed in order to explore whether there is an association between the prevalence of the Beijing MTB genotype and MDR-TB in Myanmar. Methods: A total of 265 MDR-MTB clinical isolates collected in 2010 and 2012 were subjected to spoligotyping, mycobacterial interspersed repetitive unit–variable number tandem repeat (MIRU-VNTR) analysis, single nucleotide polymorphism (SNP) typing, and drug resistance-associated gene sequencing, including rpoC to detect potential compensatory evolution. Results: Of the total MDR-MTB isolates, 79.2% (210/265) were of the Beijing genotype, the majority of which were the ‘modern’ subtype. Beijing genotype isolates were differentiated by 15-locus MIRU-VNTR and a high clustering rate (53.0%) was observed in the modern subtype. These MIRU-VNTR patterns were similar to Beijing genotype clones spreading across Russia and Central Asia. A high prevalence of katG Ser315Thr, and genetic evidence of extensive drug resistance (XDR) and pre-XDR and compensatory mutations in rpoC were observed among clustered isolates. Conclusions: MDR-MTB strains of the Beijing genotype might be spreading in Myanmar and present a major challenge to TB control in this country. Keywords: Mycobacterium tuberculosis, Multidrug-resistant TB, Beijing genotype, MIRU-VNTR, Myanmar |
url |
http://www.sciencedirect.com/science/article/pii/S1201971218344448 |
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