Global mapping of transposon location.
Transposable genetic elements are ubiquitous, yet their presence or absence at any given position within a genome can vary between individual cells, tissues, or strains. Transposable elements have profound impacts on host genomes by altering gene expression, assisting in genomic rearrangements, caus...
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2006-12-01
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Series: | PLoS Genetics |
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doaj-b1423d7f2d884bf5b529821c54959b932020-11-24T21:41:38ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042006-12-01212e21210.1371/journal.pgen.0020212Global mapping of transposon location.Abram GabrielJohannes DapprichMark KunkelDavid GreshamStephen C PrattMaitreya J DunhamTransposable genetic elements are ubiquitous, yet their presence or absence at any given position within a genome can vary between individual cells, tissues, or strains. Transposable elements have profound impacts on host genomes by altering gene expression, assisting in genomic rearrangements, causing insertional mutations, and serving as sources of phenotypic variation. Characterizing a genome's full complement of transposons requires whole genome sequencing, precluding simple studies of the impact of transposition on interindividual variation. Here, we describe a global mapping approach for identifying transposon locations in any genome, using a combination of transposon-specific DNA extraction and microarray-based comparative hybridization analysis. We use this approach to map the repertoire of endogenous transposons in different laboratory strains of Saccharomyces cerevisiae and demonstrate that transposons are a source of extensive genomic variation. We also apply this method to mapping bacterial transposon insertion sites in a yeast genomic library. This unique whole genome view of transposon location will facilitate our exploration of transposon dynamics, as well as defining bases for individual differences and adaptive potential.http://europepmc.org/articles/PMC1698948?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Abram Gabriel Johannes Dapprich Mark Kunkel David Gresham Stephen C Pratt Maitreya J Dunham |
spellingShingle |
Abram Gabriel Johannes Dapprich Mark Kunkel David Gresham Stephen C Pratt Maitreya J Dunham Global mapping of transposon location. PLoS Genetics |
author_facet |
Abram Gabriel Johannes Dapprich Mark Kunkel David Gresham Stephen C Pratt Maitreya J Dunham |
author_sort |
Abram Gabriel |
title |
Global mapping of transposon location. |
title_short |
Global mapping of transposon location. |
title_full |
Global mapping of transposon location. |
title_fullStr |
Global mapping of transposon location. |
title_full_unstemmed |
Global mapping of transposon location. |
title_sort |
global mapping of transposon location. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Genetics |
issn |
1553-7390 1553-7404 |
publishDate |
2006-12-01 |
description |
Transposable genetic elements are ubiquitous, yet their presence or absence at any given position within a genome can vary between individual cells, tissues, or strains. Transposable elements have profound impacts on host genomes by altering gene expression, assisting in genomic rearrangements, causing insertional mutations, and serving as sources of phenotypic variation. Characterizing a genome's full complement of transposons requires whole genome sequencing, precluding simple studies of the impact of transposition on interindividual variation. Here, we describe a global mapping approach for identifying transposon locations in any genome, using a combination of transposon-specific DNA extraction and microarray-based comparative hybridization analysis. We use this approach to map the repertoire of endogenous transposons in different laboratory strains of Saccharomyces cerevisiae and demonstrate that transposons are a source of extensive genomic variation. We also apply this method to mapping bacterial transposon insertion sites in a yeast genomic library. This unique whole genome view of transposon location will facilitate our exploration of transposon dynamics, as well as defining bases for individual differences and adaptive potential. |
url |
http://europepmc.org/articles/PMC1698948?pdf=render |
work_keys_str_mv |
AT abramgabriel globalmappingoftransposonlocation AT johannesdapprich globalmappingoftransposonlocation AT markkunkel globalmappingoftransposonlocation AT davidgresham globalmappingoftransposonlocation AT stephencpratt globalmappingoftransposonlocation AT maitreyajdunham globalmappingoftransposonlocation |
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1725920785365729280 |