Genus-Wide Microsatellite Primers for the Goldenrods (Solidago; Asteraceae)

Premise of the study: Microsatellite primers were developed for studies of polyploid evolution, ecological genetics, conservation genetics, and species delimitation in the genus Solidago. Methods and Results: Illumina sequencing of a shotgun library from S. gigantea</...

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Main Authors:	James B. Beck, John C. Semple, Justin M. Brull, Stacey L. Lance, Mai M. Phillips, Sara B. Hoot, Gretchen A. Meyer
Format:	Article
Language:	English
Published:	Wiley 2014-04-01
Series:	Applications in Plant Sciences
Subjects:	Asteraceae Illumina sequencing polyploidy simple sequence repeat (SSR) markers Solidago
Online Access:	http://www.bioone.org/doi/full/10.3732/apps.1300093

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spelling	doaj-b14648cfa72e48bc82f1122d27361e7b2020-11-24T23:56:27ZengWileyApplications in Plant Sciences2168-04502014-04-0124130009310.3732/apps.1300093Genus-Wide Microsatellite Primers for the Goldenrods (<i>Solidago</i>; Asteraceae)James B. Beck0John C. Semple1Justin M. Brull2Stacey L. Lance3Mai M. Phillips4Sara B. Hoot5Gretchen A. Meyer6Department of Biological Sciences, Wichita State University, 537 Hubbard Hall, Wichita, Kansas 67260 USADepartment of Biology, University of Waterloo, Waterloo, Ontario NL2 3G1, CanadaDepartment of Biological Sciences, Wichita State University, 537 Hubbard Hall, Wichita, Kansas 67260 USASavannah River Ecology Laboratory, University of Georgia, Aiken, South Carolina 29802 USAConservation and Environmental Science Program, University of Wisconsin–Milwaukee, 3209 N. Maryland Ave., Milwaukee, Wisconsin 53201 USADepartment of Biological Sciences, University of Wisconsin–Milwaukee, 3209 N. Maryland Ave., Milwaukee, Wisconsin 53201 USAField Station, University of Wisconsin–Milwaukee, 3095 Blue Goose Road, Saukville, Wisconsin 53080 USAPremise of the study: Microsatellite primers were developed for studies of polyploid evolution, ecological genetics, conservation genetics, and species delimitation in the genus <i>Solidago</i>. Methods and Results: Illumina sequencing of a shotgun library from <i>S. gigantea</i> identified ca. 1900 putative single-copy loci. Fourteen loci were subsequently shown to be amplifiable, single-copy, and variable in a broad range of <i>Solidago</i> species. Conclusions: The utility of these markers both across the genus and in herbarium specimens of a wide age range will facilitate numerous inter- and intraspecific studies in the ca. 120 <i>Solidago</i> species.http://www.bioone.org/doi/full/10.3732/apps.1300093AsteraceaeIllumina sequencingpolyploidysimple sequence repeat (SSR) markersSolidago
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	James B. Beck John C. Semple Justin M. Brull Stacey L. Lance Mai M. Phillips Sara B. Hoot Gretchen A. Meyer
spellingShingle	James B. Beck John C. Semple Justin M. Brull Stacey L. Lance Mai M. Phillips Sara B. Hoot Gretchen A. Meyer Genus-Wide Microsatellite Primers for the Goldenrods (<i>Solidago</i>; Asteraceae) Applications in Plant Sciences Asteraceae Illumina sequencing polyploidy simple sequence repeat (SSR) markers Solidago
author_facet	James B. Beck John C. Semple Justin M. Brull Stacey L. Lance Mai M. Phillips Sara B. Hoot Gretchen A. Meyer
author_sort	James B. Beck
title	Genus-Wide Microsatellite Primers for the Goldenrods (<i>Solidago</i>; Asteraceae)
title_short	Genus-Wide Microsatellite Primers for the Goldenrods (<i>Solidago</i>; Asteraceae)
title_full	Genus-Wide Microsatellite Primers for the Goldenrods (<i>Solidago</i>; Asteraceae)
title_fullStr	Genus-Wide Microsatellite Primers for the Goldenrods (<i>Solidago</i>; Asteraceae)
title_full_unstemmed	Genus-Wide Microsatellite Primers for the Goldenrods (<i>Solidago</i>; Asteraceae)
title_sort	genus-wide microsatellite primers for the goldenrods (<i>solidago</i>; asteraceae)
publisher	Wiley
series	Applications in Plant Sciences
issn	2168-0450
publishDate	2014-04-01
description	Premise of the study: Microsatellite primers were developed for studies of polyploid evolution, ecological genetics, conservation genetics, and species delimitation in the genus <i>Solidago</i>. Methods and Results: Illumina sequencing of a shotgun library from <i>S. gigantea</i> identified ca. 1900 putative single-copy loci. Fourteen loci were subsequently shown to be amplifiable, single-copy, and variable in a broad range of <i>Solidago</i> species. Conclusions: The utility of these markers both across the genus and in herbarium specimens of a wide age range will facilitate numerous inter- and intraspecific studies in the ca. 120 <i>Solidago</i> species.
topic	Asteraceae Illumina sequencing polyploidy simple sequence repeat (SSR) markers Solidago
url	http://www.bioone.org/doi/full/10.3732/apps.1300093
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Genus-Wide Microsatellite Primers for the Goldenrods (<i>Solidago</i>; Asteraceae)

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