Quest for An Ideal, Simple and Cost-Effective Stain for Morphological Assessment of Sperms
Background: Recent alarming trends of a substantial rise in the number of cases of infertility with as many as 30-40% being attributed to male-factor associated causes have created a need for further studies and advancements in semen analysis. Despite the focus on semen analysis over the years,...
Main Authors: | , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
JCDR Research and Publications Private Limited
2015-10-01
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Series: | Journal of Clinical and Diagnostic Research |
Subjects: | |
Online Access: | https://jcdr.net/articles/PDF/6655/13270_CE[Ra1]New_F(AK)_PF1(EKAK)_PFA(AK)_PF2(PAG).pdf |
Summary: | Background: Recent alarming trends of a substantial rise in the
number of cases of infertility with as many as 30-40% being
attributed to male-factor associated causes have created a
need for further studies and advancements in semen analysis.
Despite the focus on semen analysis over the years, assessment
of sperm morphology has not been given due importance
although it is a simple, standard and baseline diagnostic
modality. It can be used to predict the need and outcome of
Artificial Reproductive Techniques such as Invitro Fertilization,
Gamete Intra Fallopian Tube Transfer and Intra Cytoplasmic
Sperm Injection.
Aim: To find the ideal, simple and cost-effective basic stain
for assessment of sperm morphology in a rural tertiary care
set- up where advanced equipment for assessment of sperm
morphometry are inaccessible.
Materials and Methods: An updated way of determining sperm
shape is called the Kruger’s strict morphology method. Keeping
this as the standard criterion, we studied semen samples of 62
healthy male subjects using four basic staining techniques and
the consensus of four independent observers was tabulated.
Results: We found that Haematoxylin and Eosin stain was the
best stain for assessment of sperm head morphology. Rapid
Papanicolau stain was the most ideal, simple and cost-effective
stain for overall assessment of sperm morphology.
Conclusion: Sperm morphology assessment remains the
baseline necessity for the diagnosis and management of male
factor associated infertility when advanced techniques are
unavailable, inaccessible or unaffordable. |
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ISSN: | 2249-782X 0973-709X |