Effects of Cytokinin and Auxin on In Vitro Organ Development and Plumbagin Content of Drosera peltata Thunb.
A rapid propagation and plumbagin production of Drosera peltata was developed and investigated. The research aims to study the effects of cytokinins and auxins on organ development and plumbagin production from shoot tip cultures. In vitro generated shoot tips were cultured on the semi-solid 1/2 MS...
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doaj-b16262cac07a424ab7ab0c1f0c8a2ac32020-11-24T22:01:47ZengUniversitas BrawijayaAGRIVITA Journal of Agricultural Science0126-05372477-85162018-08-0140341542410.17503/agrivita.v40i0.1276411Effects of Cytokinin and Auxin on In Vitro Organ Development and Plumbagin Content of Drosera peltata Thunb.Thanakorn Wongsa0Phithak Inthima1Maliwan Nakkuntod2Duangporn Premjet3Anupan Kongbangkerd4Plant Tissue Culture Research Unit, Department of Biology, Faculty of Science, Naresuan University, Muang, Phitsanulok 65000 ThailandPlant Tissue Culture Research Unit, Department of Biology, Faculty of Science, Naresuan University, Muang, Phitsanulok 65000 ThailandPlant Molecular Biology Research Unit, Department of Biology, Faculty of Science, Naresuan University, Muang, Phitsanulok 65000 ThailandDepartment of Agricultural Science, Faculty of Agriculture, Natural Resources, and Environment, Naresuan University, Muang, Phitsanulok 65000 ThailandPlant Tissue Culture Research Unit, Department of Biology, Faculty of Science, Naresuan University, Muang, Phitsanulok 65000 ThailandA rapid propagation and plumbagin production of Drosera peltata was developed and investigated. The research aims to study the effects of cytokinins and auxins on organ development and plumbagin production from shoot tip cultures. In vitro generated shoot tips were cultured on the semi-solid 1/2 MS medium containing 3.0% sucrose, 2.0% gelrite, and 0.1, 0.5, 1.0, and 2.0 mg L–1cytokinins (BA, Kn, TDZ) and auxins (IAA, IBA, NAA and 2,4-D) for 12 weeks. The highest number of shoots (12.0 ± 1.2) was formed on the medium containing 1.0 mg L–1 TDZ, which was four-fold higher than in the control. Meanwhile, the highest number of roots per explant (9.4 ± 1.3) and rhizomes per explant (8.1 ± 0.8) were formed on the medium containing 2.0 mg L–1 NAA. The best callus induction (100%) was found on the 0.5–2.0 mg L–1 2,4-D-containing medium. Moreover, the highest plumbagin content (12.04 mg g–1 DW) was detected from shoots regenerated on the 0.1 mg L–1 BA-containing medium, which was approximately two-fold higher than that in the control. The study is efficient for organs induction and enhances plumbagin content from shoot tip explants of D. peltata.http://agrivita.ub.ac.id/index.php/agrivita/article/view/1276DroseraMorphogenesisPlant growth regulatorPlumbagin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Thanakorn Wongsa Phithak Inthima Maliwan Nakkuntod Duangporn Premjet Anupan Kongbangkerd |
spellingShingle |
Thanakorn Wongsa Phithak Inthima Maliwan Nakkuntod Duangporn Premjet Anupan Kongbangkerd Effects of Cytokinin and Auxin on In Vitro Organ Development and Plumbagin Content of Drosera peltata Thunb. AGRIVITA Journal of Agricultural Science Drosera Morphogenesis Plant growth regulator Plumbagin |
author_facet |
Thanakorn Wongsa Phithak Inthima Maliwan Nakkuntod Duangporn Premjet Anupan Kongbangkerd |
author_sort |
Thanakorn Wongsa |
title |
Effects of Cytokinin and Auxin on In Vitro Organ Development and Plumbagin Content of Drosera peltata Thunb. |
title_short |
Effects of Cytokinin and Auxin on In Vitro Organ Development and Plumbagin Content of Drosera peltata Thunb. |
title_full |
Effects of Cytokinin and Auxin on In Vitro Organ Development and Plumbagin Content of Drosera peltata Thunb. |
title_fullStr |
Effects of Cytokinin and Auxin on In Vitro Organ Development and Plumbagin Content of Drosera peltata Thunb. |
title_full_unstemmed |
Effects of Cytokinin and Auxin on In Vitro Organ Development and Plumbagin Content of Drosera peltata Thunb. |
title_sort |
effects of cytokinin and auxin on in vitro organ development and plumbagin content of drosera peltata thunb. |
publisher |
Universitas Brawijaya |
series |
AGRIVITA Journal of Agricultural Science |
issn |
0126-0537 2477-8516 |
publishDate |
2018-08-01 |
description |
A rapid propagation and plumbagin production of Drosera peltata was developed and investigated. The research aims to study the effects of cytokinins and auxins on organ development and plumbagin production from shoot tip cultures. In vitro generated shoot tips were cultured on the semi-solid 1/2 MS medium containing 3.0% sucrose, 2.0% gelrite, and 0.1, 0.5, 1.0, and 2.0 mg L–1cytokinins (BA, Kn, TDZ) and auxins (IAA, IBA, NAA and 2,4-D) for 12 weeks. The highest number of shoots (12.0 ± 1.2) was formed on the medium containing 1.0 mg L–1 TDZ, which was four-fold higher than in the control. Meanwhile, the highest number of roots per explant (9.4 ± 1.3) and rhizomes per explant (8.1 ± 0.8) were formed on the medium containing 2.0 mg L–1 NAA. The best callus induction (100%) was found on the 0.5–2.0 mg L–1 2,4-D-containing medium. Moreover, the highest plumbagin content (12.04 mg g–1 DW) was detected from shoots regenerated on the 0.1 mg L–1 BA-containing medium, which was approximately two-fold higher than that in the control. The study is efficient for organs induction and enhances plumbagin content from shoot tip explants of D. peltata. |
topic |
Drosera Morphogenesis Plant growth regulator Plumbagin |
url |
http://agrivita.ub.ac.id/index.php/agrivita/article/view/1276 |
work_keys_str_mv |
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