TM4SF1 is a potential target for anti-invasion and metastasis in ovarian cancer
Abstract Background Patients with ovarian cancer commonly have a poor prognosis, owing to its invasiveness and distant metastasis. Studies have found TM4SF1 participates in regulating tumor cell invasion and migration. Therefore, it is expected to become a target for anti-invasion and metastasis in...
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doaj-b1783df3f5dd4b4ea0031634d7e119022020-11-25T02:25:11ZengBMCBMC Cancer1471-24072019-03-0119111210.1186/s12885-019-5417-7TM4SF1 is a potential target for anti-invasion and metastasis in ovarian cancerCaiyun Gao0Hongyu Yao1Huimin Liu2Yanying Feng3Zhijun Yang4Department of Gynecologic Oncology, Affiliated Tumor Hospital of Guangxi Medical UniversityDepartment of Gynecologic Oncology, Affiliated Tumor Hospital of Guangxi Medical UniversityDepartment of Gynecologic Oncology, Affiliated Tumor Hospital of Guangxi Medical UniversityDepartments of Electrocardiogram, Affiliated Tumor Hospital of Guangxi Medical UniversityDepartment of Gynecologic Oncology, Affiliated Tumor Hospital of Guangxi Medical UniversityAbstract Background Patients with ovarian cancer commonly have a poor prognosis, owing to its invasiveness and distant metastasis. Studies have found TM4SF1 participates in regulating tumor cell invasion and migration. Therefore, it is expected to become a target for anti-invasion and metastasis in ovarian cancer. Methods The expression of TM4SF1 in normal ovarian epithelial tissues, benign ovarian tumor tissues, primary foci of epithelial ovarian cancer and the matched lymph mode metastatic foci was detected using immunohistochemistry to analyze its association with prognosis. The expression of TM4SF1 in HO8910PM, SKOV3 was inhibited using RNAi, and the growth, proliferation, migration, invasion abilities of HO8910PM and SKOV3 cells and the growth of xenograft tumors in nude mice were examined. Results (1) The positive expression rate of TM4SF1 protein in epithelial ovarian cancer tissues (90.90%) was higher than that in benign ovarian tumor tissues (65.22%) and normal ovarian epithelial tissues (31.25%), and both differences were significant (P < 0.05). The expression of TM4SF1 protein was positive in all metastatic lymph node foci and matched primary foci (100%). (2) The level of TM4SF1 protein expression was positively correlated with the International Federation of Gynecology and Obstetrics (FIGO) stage and histological grade. However, The positive TM4SF1 protein expression was not an independent factor of prognosis (P > 0.05). (3) Silencing TM4SF1 expression did not affect growth, proliferation, or cell cycle distribution but inhibited the migration and invasion abilities of HO8910PM and SKOV3 cells. Silencing TM4SF1 expression inhibited the growth of xenograft tumors in nude mice. Conclusion TM4SF1 is a potential target for anti-invasion and metastasis in ovarian cancer.http://link.springer.com/article/10.1186/s12885-019-5417-7TM4SF1Ovarian cancerInvasionMetastasisTarget |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Caiyun Gao Hongyu Yao Huimin Liu Yanying Feng Zhijun Yang |
spellingShingle |
Caiyun Gao Hongyu Yao Huimin Liu Yanying Feng Zhijun Yang TM4SF1 is a potential target for anti-invasion and metastasis in ovarian cancer BMC Cancer TM4SF1 Ovarian cancer Invasion Metastasis Target |
author_facet |
Caiyun Gao Hongyu Yao Huimin Liu Yanying Feng Zhijun Yang |
author_sort |
Caiyun Gao |
title |
TM4SF1 is a potential target for anti-invasion and metastasis in ovarian cancer |
title_short |
TM4SF1 is a potential target for anti-invasion and metastasis in ovarian cancer |
title_full |
TM4SF1 is a potential target for anti-invasion and metastasis in ovarian cancer |
title_fullStr |
TM4SF1 is a potential target for anti-invasion and metastasis in ovarian cancer |
title_full_unstemmed |
TM4SF1 is a potential target for anti-invasion and metastasis in ovarian cancer |
title_sort |
tm4sf1 is a potential target for anti-invasion and metastasis in ovarian cancer |
publisher |
BMC |
series |
BMC Cancer |
issn |
1471-2407 |
publishDate |
2019-03-01 |
description |
Abstract Background Patients with ovarian cancer commonly have a poor prognosis, owing to its invasiveness and distant metastasis. Studies have found TM4SF1 participates in regulating tumor cell invasion and migration. Therefore, it is expected to become a target for anti-invasion and metastasis in ovarian cancer. Methods The expression of TM4SF1 in normal ovarian epithelial tissues, benign ovarian tumor tissues, primary foci of epithelial ovarian cancer and the matched lymph mode metastatic foci was detected using immunohistochemistry to analyze its association with prognosis. The expression of TM4SF1 in HO8910PM, SKOV3 was inhibited using RNAi, and the growth, proliferation, migration, invasion abilities of HO8910PM and SKOV3 cells and the growth of xenograft tumors in nude mice were examined. Results (1) The positive expression rate of TM4SF1 protein in epithelial ovarian cancer tissues (90.90%) was higher than that in benign ovarian tumor tissues (65.22%) and normal ovarian epithelial tissues (31.25%), and both differences were significant (P < 0.05). The expression of TM4SF1 protein was positive in all metastatic lymph node foci and matched primary foci (100%). (2) The level of TM4SF1 protein expression was positively correlated with the International Federation of Gynecology and Obstetrics (FIGO) stage and histological grade. However, The positive TM4SF1 protein expression was not an independent factor of prognosis (P > 0.05). (3) Silencing TM4SF1 expression did not affect growth, proliferation, or cell cycle distribution but inhibited the migration and invasion abilities of HO8910PM and SKOV3 cells. Silencing TM4SF1 expression inhibited the growth of xenograft tumors in nude mice. Conclusion TM4SF1 is a potential target for anti-invasion and metastasis in ovarian cancer. |
topic |
TM4SF1 Ovarian cancer Invasion Metastasis Target |
url |
http://link.springer.com/article/10.1186/s12885-019-5417-7 |
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