Transformation of medicinal plants using Agrobacterium tumefaciens

• Main Authors: Katarzyna Bandurska, Agnieszka Berdowska, Małgorzata Król
• Format: Article
• Language: English
• Published: Index Copernicus International S.A. 2016-12-01
• Series: Postępy Higieny i Medycyny Doświadczalnej
• Subjects: transformacja genetyczna roślin leczniczych; Agrobacterium tumefaciens; produkcja substancji biologicznie czynnych; genetic transformation of medicinal plants; production of biologically active substances
• Online Access: http://phmd.pl/gicid/01.3001.0009.6900

For many years attempts are made to develop efficient methods for transformation of medicinal plants via Agrobacterium tumefaciens. It is a soil bacteria which possess a natural ability to infect plants in places of injures which results in arise of cancerous growths (crown gall). This is possible thanks a transfer of fragment of Ti plasmid into plant cells and stable integration with a plant genome. Efficiency of medicinal plant transformation depends on many factors for example: Agrobacterium strain, methods and procedures of transformation as well as on plant species, type and age of the explants and regeneration conditions. The main goal of plant transformation is to increase the amount of naturally occurring bioactive compounds and the production of biopharmaceuticals. Genetic plant transformation via bacteria of the genus Agrobacterium is a complex process which requires detailed analysis of incorporated transgene expression and occurs only in the case when the plant cell acquires the ability to regenerate. In many cases, the regeneration efficiency observed in medicinal plants are inefficient after applied transformation procedures. To date there have been attempts of genetic transformation by using A. tumefaciens of medicinal plants belonging to the families: Apocynaceae, Araceae, Araliaceae, Asphodelaceae, Asteraceae, Begoniaceae, Crassulaceae, Fabaceae, Lamiaceae, Linaceae, Papaveraceae, Plantaginaceae, Scrophulariaceae and Solanaceae.