A lover and a fighter: the genome sequence of an entomopathogenic nematode Heterorhabditis bacteriophora.
Heterorhabditis bacteriophora are entomopathogenic nematodes that have evolved a mutualism with Photorhabdus luminescens bacteria to function as highly virulent insect pathogens. The nematode provides a safe harbor for intestinal symbionts in soil and delivers the symbiotic bacteria into the insect...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2013-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3715494?pdf=render |
id |
doaj-b17f50e54e904206956eeee9f0d43dcc |
---|---|
record_format |
Article |
spelling |
doaj-b17f50e54e904206956eeee9f0d43dcc2020-11-24T20:50:08ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0187e6961810.1371/journal.pone.0069618A lover and a fighter: the genome sequence of an entomopathogenic nematode Heterorhabditis bacteriophora.Xiaodong BaiByron J AdamsTodd A CicheSandra CliftonRandy GauglerKwi-suk KimJohn SpiethPaul W SternbergRichard K WilsonParwinder S GrewalHeterorhabditis bacteriophora are entomopathogenic nematodes that have evolved a mutualism with Photorhabdus luminescens bacteria to function as highly virulent insect pathogens. The nematode provides a safe harbor for intestinal symbionts in soil and delivers the symbiotic bacteria into the insect blood. The symbiont provides virulence and toxins, metabolites essential for nematode reproduction, and antibiotic preservation of the insect cadaver. Approximately half of the 21,250 putative protein coding genes identified in the 77 Mbp high quality draft H. bacteriophora genome sequence were novel proteins of unknown function lacking homologs in Caenorhabditis elegans or any other sequenced organisms. Similarly, 317 of the 603 predicted secreted proteins are novel with unknown function in addition to 19 putative peptidases, 9 peptidase inhibitors and 7 C-type lectins that may function in interactions with insect hosts or bacterial symbionts. The 134 proteins contained mariner transposase domains, of which there are none in C. elegans, suggesting an invasion and expansion of mariner transposons in H. bacteriophora. Fewer Kyoto Encyclopedia of Genes and Genomes Orthologies in almost all metabolic categories were detected in the genome compared with 9 other sequenced nematode genomes, which may reflect dependence on the symbiont or insect host for these functions. The H. bacteriophora genome sequence will greatly facilitate genetics, genomics and evolutionary studies to gain fundamental knowledge of nematode parasitism and mutualism. It also elevates the utility of H. bacteriophora as a bridge species between vertebrate parasitic nematodes and the C. elegans model.http://europepmc.org/articles/PMC3715494?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiaodong Bai Byron J Adams Todd A Ciche Sandra Clifton Randy Gaugler Kwi-suk Kim John Spieth Paul W Sternberg Richard K Wilson Parwinder S Grewal |
spellingShingle |
Xiaodong Bai Byron J Adams Todd A Ciche Sandra Clifton Randy Gaugler Kwi-suk Kim John Spieth Paul W Sternberg Richard K Wilson Parwinder S Grewal A lover and a fighter: the genome sequence of an entomopathogenic nematode Heterorhabditis bacteriophora. PLoS ONE |
author_facet |
Xiaodong Bai Byron J Adams Todd A Ciche Sandra Clifton Randy Gaugler Kwi-suk Kim John Spieth Paul W Sternberg Richard K Wilson Parwinder S Grewal |
author_sort |
Xiaodong Bai |
title |
A lover and a fighter: the genome sequence of an entomopathogenic nematode Heterorhabditis bacteriophora. |
title_short |
A lover and a fighter: the genome sequence of an entomopathogenic nematode Heterorhabditis bacteriophora. |
title_full |
A lover and a fighter: the genome sequence of an entomopathogenic nematode Heterorhabditis bacteriophora. |
title_fullStr |
A lover and a fighter: the genome sequence of an entomopathogenic nematode Heterorhabditis bacteriophora. |
title_full_unstemmed |
A lover and a fighter: the genome sequence of an entomopathogenic nematode Heterorhabditis bacteriophora. |
title_sort |
lover and a fighter: the genome sequence of an entomopathogenic nematode heterorhabditis bacteriophora. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
Heterorhabditis bacteriophora are entomopathogenic nematodes that have evolved a mutualism with Photorhabdus luminescens bacteria to function as highly virulent insect pathogens. The nematode provides a safe harbor for intestinal symbionts in soil and delivers the symbiotic bacteria into the insect blood. The symbiont provides virulence and toxins, metabolites essential for nematode reproduction, and antibiotic preservation of the insect cadaver. Approximately half of the 21,250 putative protein coding genes identified in the 77 Mbp high quality draft H. bacteriophora genome sequence were novel proteins of unknown function lacking homologs in Caenorhabditis elegans or any other sequenced organisms. Similarly, 317 of the 603 predicted secreted proteins are novel with unknown function in addition to 19 putative peptidases, 9 peptidase inhibitors and 7 C-type lectins that may function in interactions with insect hosts or bacterial symbionts. The 134 proteins contained mariner transposase domains, of which there are none in C. elegans, suggesting an invasion and expansion of mariner transposons in H. bacteriophora. Fewer Kyoto Encyclopedia of Genes and Genomes Orthologies in almost all metabolic categories were detected in the genome compared with 9 other sequenced nematode genomes, which may reflect dependence on the symbiont or insect host for these functions. The H. bacteriophora genome sequence will greatly facilitate genetics, genomics and evolutionary studies to gain fundamental knowledge of nematode parasitism and mutualism. It also elevates the utility of H. bacteriophora as a bridge species between vertebrate parasitic nematodes and the C. elegans model. |
url |
http://europepmc.org/articles/PMC3715494?pdf=render |
work_keys_str_mv |
AT xiaodongbai aloverandafighterthegenomesequenceofanentomopathogenicnematodeheterorhabditisbacteriophora AT byronjadams aloverandafighterthegenomesequenceofanentomopathogenicnematodeheterorhabditisbacteriophora AT toddaciche aloverandafighterthegenomesequenceofanentomopathogenicnematodeheterorhabditisbacteriophora AT sandraclifton aloverandafighterthegenomesequenceofanentomopathogenicnematodeheterorhabditisbacteriophora AT randygaugler aloverandafighterthegenomesequenceofanentomopathogenicnematodeheterorhabditisbacteriophora AT kwisukkim aloverandafighterthegenomesequenceofanentomopathogenicnematodeheterorhabditisbacteriophora AT johnspieth aloverandafighterthegenomesequenceofanentomopathogenicnematodeheterorhabditisbacteriophora AT paulwsternberg aloverandafighterthegenomesequenceofanentomopathogenicnematodeheterorhabditisbacteriophora AT richardkwilson aloverandafighterthegenomesequenceofanentomopathogenicnematodeheterorhabditisbacteriophora AT parwindersgrewal aloverandafighterthegenomesequenceofanentomopathogenicnematodeheterorhabditisbacteriophora AT xiaodongbai loverandafighterthegenomesequenceofanentomopathogenicnematodeheterorhabditisbacteriophora AT byronjadams loverandafighterthegenomesequenceofanentomopathogenicnematodeheterorhabditisbacteriophora AT toddaciche loverandafighterthegenomesequenceofanentomopathogenicnematodeheterorhabditisbacteriophora AT sandraclifton loverandafighterthegenomesequenceofanentomopathogenicnematodeheterorhabditisbacteriophora AT randygaugler loverandafighterthegenomesequenceofanentomopathogenicnematodeheterorhabditisbacteriophora AT kwisukkim loverandafighterthegenomesequenceofanentomopathogenicnematodeheterorhabditisbacteriophora AT johnspieth loverandafighterthegenomesequenceofanentomopathogenicnematodeheterorhabditisbacteriophora AT paulwsternberg loverandafighterthegenomesequenceofanentomopathogenicnematodeheterorhabditisbacteriophora AT richardkwilson loverandafighterthegenomesequenceofanentomopathogenicnematodeheterorhabditisbacteriophora AT parwindersgrewal loverandafighterthegenomesequenceofanentomopathogenicnematodeheterorhabditisbacteriophora |
_version_ |
1716804543871713280 |