Multiparameter Analysis of Human Bone Marrow Stromal Cells Identifies Distinct Immunomodulatory and Differentiation-Competent Subtypes
Bone marrow stromal cells (BMSCs, also called bone-marrow-derived mesenchymal stromal cells) provide hematopoietic support and immunoregulation and contain a stem cell fraction capable of skeletogenic differentiation. We used immortalized human BMSC clonal lines for multi-level analysis of functiona...
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doaj-b181f4a8235e4ef1b443cf855df7fef82020-11-24T22:25:08ZengElsevierStem Cell Reports2213-67112015-06-01461004101510.1016/j.stemcr.2015.05.005Multiparameter Analysis of Human Bone Marrow Stromal Cells Identifies Distinct Immunomodulatory and Differentiation-Competent SubtypesSally James0James Fox1Farinaz Afsari2Jennifer Lee3Sally Clough4Charlotte Knight5James Ashmore6Peter Ashton7Olivier Preham8Martin Hoogduijn9Raquel De Almeida Rocha Ponzoni10Y. Hancock11Mark Coles12Paul Genever13Department of Biology, University of York, York YO10 5DD, UKDepartment of Biology, University of York, York YO10 5DD, UKDepartment of Biology, University of York, York YO10 5DD, UKDepartment of Biology, University of York, York YO10 5DD, UKDepartment of Biology, University of York, York YO10 5DD, UKDepartment of Biology, University of York, York YO10 5DD, UKDepartment of Biology, University of York, York YO10 5DD, UKDepartment of Biology, University of York, York YO10 5DD, UKDepartment of Biology, University of York, York YO10 5DD, UKErasmus Medical Centre, Dr. Molewaterplein 50, Rotterdam 3015 GE, the NetherlandsDepartment of Physics, University of York, York YO10 5DD, UKDepartment of Physics, University of York, York YO10 5DD, UKDepartment of Biology, University of York, York YO10 5DD, UKDepartment of Biology, University of York, York YO10 5DD, UKBone marrow stromal cells (BMSCs, also called bone-marrow-derived mesenchymal stromal cells) provide hematopoietic support and immunoregulation and contain a stem cell fraction capable of skeletogenic differentiation. We used immortalized human BMSC clonal lines for multi-level analysis of functional markers for BMSC subsets. All clones expressed typical BMSC cell-surface antigens; however, clones with trilineage differentiation capacity exhibited enhanced vascular interaction gene sets, whereas non-differentiating clones were uniquely CD317 positive with significantly enriched immunomodulatory transcriptional networks and high IL-7 production. IL-7 lineage tracing and CD317 immunolocalization confirmed the existence of a rare non-differentiating BMSC subtype, distinct from Cxcl12-DsRed+ perivascular stromal cells in vivo. Colony-forming CD317+ IL-7hi cells, identified at ∼1%–3% frequency in heterogeneous human BMSC fractions, were found to have the same biomolecular profile as non-differentiating BMSC clones using Raman spectroscopy. Distinct functional identities can be assigned to BMSC subpopulations, which are likely to have specific roles in immune control, lymphopoiesis, and bone homeostasis.http://www.sciencedirect.com/science/article/pii/S2213671115001484 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sally James James Fox Farinaz Afsari Jennifer Lee Sally Clough Charlotte Knight James Ashmore Peter Ashton Olivier Preham Martin Hoogduijn Raquel De Almeida Rocha Ponzoni Y. Hancock Mark Coles Paul Genever |
spellingShingle |
Sally James James Fox Farinaz Afsari Jennifer Lee Sally Clough Charlotte Knight James Ashmore Peter Ashton Olivier Preham Martin Hoogduijn Raquel De Almeida Rocha Ponzoni Y. Hancock Mark Coles Paul Genever Multiparameter Analysis of Human Bone Marrow Stromal Cells Identifies Distinct Immunomodulatory and Differentiation-Competent Subtypes Stem Cell Reports |
author_facet |
Sally James James Fox Farinaz Afsari Jennifer Lee Sally Clough Charlotte Knight James Ashmore Peter Ashton Olivier Preham Martin Hoogduijn Raquel De Almeida Rocha Ponzoni Y. Hancock Mark Coles Paul Genever |
author_sort |
Sally James |
title |
Multiparameter Analysis of Human Bone Marrow Stromal Cells Identifies Distinct Immunomodulatory and Differentiation-Competent Subtypes |
title_short |
Multiparameter Analysis of Human Bone Marrow Stromal Cells Identifies Distinct Immunomodulatory and Differentiation-Competent Subtypes |
title_full |
Multiparameter Analysis of Human Bone Marrow Stromal Cells Identifies Distinct Immunomodulatory and Differentiation-Competent Subtypes |
title_fullStr |
Multiparameter Analysis of Human Bone Marrow Stromal Cells Identifies Distinct Immunomodulatory and Differentiation-Competent Subtypes |
title_full_unstemmed |
Multiparameter Analysis of Human Bone Marrow Stromal Cells Identifies Distinct Immunomodulatory and Differentiation-Competent Subtypes |
title_sort |
multiparameter analysis of human bone marrow stromal cells identifies distinct immunomodulatory and differentiation-competent subtypes |
publisher |
Elsevier |
series |
Stem Cell Reports |
issn |
2213-6711 |
publishDate |
2015-06-01 |
description |
Bone marrow stromal cells (BMSCs, also called bone-marrow-derived mesenchymal stromal cells) provide hematopoietic support and immunoregulation and contain a stem cell fraction capable of skeletogenic differentiation. We used immortalized human BMSC clonal lines for multi-level analysis of functional markers for BMSC subsets. All clones expressed typical BMSC cell-surface antigens; however, clones with trilineage differentiation capacity exhibited enhanced vascular interaction gene sets, whereas non-differentiating clones were uniquely CD317 positive with significantly enriched immunomodulatory transcriptional networks and high IL-7 production. IL-7 lineage tracing and CD317 immunolocalization confirmed the existence of a rare non-differentiating BMSC subtype, distinct from Cxcl12-DsRed+ perivascular stromal cells in vivo. Colony-forming CD317+ IL-7hi cells, identified at ∼1%–3% frequency in heterogeneous human BMSC fractions, were found to have the same biomolecular profile as non-differentiating BMSC clones using Raman spectroscopy. Distinct functional identities can be assigned to BMSC subpopulations, which are likely to have specific roles in immune control, lymphopoiesis, and bone homeostasis. |
url |
http://www.sciencedirect.com/science/article/pii/S2213671115001484 |
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