H2S promotes developmental brain angiogenesis via the NOS/NO pathway in zebrafish
Background Hydrogen sulphide (H2S) is considered as the third member of the gasotransmitter family, along with nitric oxide (NO) and carbon monoxide. H2S has been reported to induce angiogenesis by promoting the growth, migration and tube-like structure formation of endothelial cells. Those studies...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMJ Publishing Group
|
Series: | Stroke and Vascular Neurology |
Online Access: | https://svn.bmj.com/content/early/2020/11/26/svn-2020-000584.full |
id |
doaj-b1aa9706e7bb42ddadd602ba73825086 |
---|---|
record_format |
Article |
spelling |
doaj-b1aa9706e7bb42ddadd602ba738250862020-12-03T03:00:02ZengBMJ Publishing GroupStroke and Vascular Neurology2059-869610.1136/svn-2020-000584H2S promotes developmental brain angiogenesis via the NOS/NO pathway in zebrafishFei Wang0Chen Liu1Weiqing Jiang2Mingzhu Deng3Xiao Ren4Yilin Fan5Jiulin Du6Yonggang Wang7Department of Neurology, Shanghai Jiao Tong University School of Medicine Affiliated Renji Hospital, Shanghai, China1Imperial College London, UKDepartment of Neurology, Shanghai Jiao Tong University School of Medicine Affiliated Renji Hospital, Shanghai, ChinaDepartment of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Neurology, Shanghai Jiao Tong University School of Medicine Affiliated Renji Hospital, Shanghai, ChinaDepartment of Neurology, Shanghai Jiao Tong University School of Medicine Affiliated Renji Hospital, Shanghai, ChinaInstitute of Neuroscience, State Key Laboratory of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, ChinaDepartment of Neurology, Shanghai Jiao Tong University School of Medicine Affiliated Renji Hospital, Shanghai, ChinaBackground Hydrogen sulphide (H2S) is considered as the third member of the gasotransmitter family, along with nitric oxide (NO) and carbon monoxide. H2S has been reported to induce angiogenesis by promoting the growth, migration and tube-like structure formation of endothelial cells. Those studies were conducted in conditions of cell culture, mouse Matrigel plug assay model, rat wound healing model or rat hindlimb ischaemia model. Recent in vivo studies showed the physiological importance of H2S in muscle angiogenesis. However, the importance of endogenous H2S for brain angiogenesis during development remains unknown. We therefore aimed at determining the role of H2S in brain vascular development.Methods and results Both knockdown and knockout of H2S-producing enzymes, cystathionine β-synthase (cbs) and cystathionine γ-lyase (cth), using morpholino oligonucleotides and clustered regularly interspaced short palindromic repeats/Cas9-mediated mutation, impaired brain vascular development of larval zebrafish. Incubation with the slow-releasing H2S donor GYY4137 alleviated the defects of brain vascular development in cbs and cth morphants. Quantitative analysis of the midbrain vascular network showed that H2S enhances angiogenesis without affecting the topological structure of the brain vasculature. Mechanically, nitric oxide synthase 2a (nos2a) expression and NO production were decreased in both cbs and cth morphants. Overexpression of nos2a by coinjection of cbs or cth MO with full-length zebrafish nos2a mRNA alleviated the brain vascular developmental defects in cbs and cth morphants.Conclusion We conclude that H2S promotes brain developmental angiogenesis via the NOS/NO pathway in zebrafish.https://svn.bmj.com/content/early/2020/11/26/svn-2020-000584.full |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fei Wang Chen Liu Weiqing Jiang Mingzhu Deng Xiao Ren Yilin Fan Jiulin Du Yonggang Wang |
spellingShingle |
Fei Wang Chen Liu Weiqing Jiang Mingzhu Deng Xiao Ren Yilin Fan Jiulin Du Yonggang Wang H2S promotes developmental brain angiogenesis via the NOS/NO pathway in zebrafish Stroke and Vascular Neurology |
author_facet |
Fei Wang Chen Liu Weiqing Jiang Mingzhu Deng Xiao Ren Yilin Fan Jiulin Du Yonggang Wang |
author_sort |
Fei Wang |
title |
H2S promotes developmental brain angiogenesis via the NOS/NO pathway in zebrafish |
title_short |
H2S promotes developmental brain angiogenesis via the NOS/NO pathway in zebrafish |
title_full |
H2S promotes developmental brain angiogenesis via the NOS/NO pathway in zebrafish |
title_fullStr |
H2S promotes developmental brain angiogenesis via the NOS/NO pathway in zebrafish |
title_full_unstemmed |
H2S promotes developmental brain angiogenesis via the NOS/NO pathway in zebrafish |
title_sort |
h2s promotes developmental brain angiogenesis via the nos/no pathway in zebrafish |
publisher |
BMJ Publishing Group |
series |
Stroke and Vascular Neurology |
issn |
2059-8696 |
description |
Background Hydrogen sulphide (H2S) is considered as the third member of the gasotransmitter family, along with nitric oxide (NO) and carbon monoxide. H2S has been reported to induce angiogenesis by promoting the growth, migration and tube-like structure formation of endothelial cells. Those studies were conducted in conditions of cell culture, mouse Matrigel plug assay model, rat wound healing model or rat hindlimb ischaemia model. Recent in vivo studies showed the physiological importance of H2S in muscle angiogenesis. However, the importance of endogenous H2S for brain angiogenesis during development remains unknown. We therefore aimed at determining the role of H2S in brain vascular development.Methods and results Both knockdown and knockout of H2S-producing enzymes, cystathionine β-synthase (cbs) and cystathionine γ-lyase (cth), using morpholino oligonucleotides and clustered regularly interspaced short palindromic repeats/Cas9-mediated mutation, impaired brain vascular development of larval zebrafish. Incubation with the slow-releasing H2S donor GYY4137 alleviated the defects of brain vascular development in cbs and cth morphants. Quantitative analysis of the midbrain vascular network showed that H2S enhances angiogenesis without affecting the topological structure of the brain vasculature. Mechanically, nitric oxide synthase 2a (nos2a) expression and NO production were decreased in both cbs and cth morphants. Overexpression of nos2a by coinjection of cbs or cth MO with full-length zebrafish nos2a mRNA alleviated the brain vascular developmental defects in cbs and cth morphants.Conclusion We conclude that H2S promotes brain developmental angiogenesis via the NOS/NO pathway in zebrafish. |
url |
https://svn.bmj.com/content/early/2020/11/26/svn-2020-000584.full |
work_keys_str_mv |
AT feiwang h2spromotesdevelopmentalbrainangiogenesisviathenosnopathwayinzebrafish AT chenliu h2spromotesdevelopmentalbrainangiogenesisviathenosnopathwayinzebrafish AT weiqingjiang h2spromotesdevelopmentalbrainangiogenesisviathenosnopathwayinzebrafish AT mingzhudeng h2spromotesdevelopmentalbrainangiogenesisviathenosnopathwayinzebrafish AT xiaoren h2spromotesdevelopmentalbrainangiogenesisviathenosnopathwayinzebrafish AT yilinfan h2spromotesdevelopmentalbrainangiogenesisviathenosnopathwayinzebrafish AT jiulindu h2spromotesdevelopmentalbrainangiogenesisviathenosnopathwayinzebrafish AT yonggangwang h2spromotesdevelopmentalbrainangiogenesisviathenosnopathwayinzebrafish |
_version_ |
1724401658008436736 |