Carbon Based Electrodes Modified with Horseradish Peroxidase Immobilized in Conducting Polymers for Acetaminophen Analysis

The development and optimization of new biosensors with horseradish peroxidase immobilized in carbon nanotubes-polyethyleneimine or polypyrrole nanocomposite film at the surface of two types of transducer is described. The amperometric detection of acetaminophen was carried out at −0.2 V versus Ag/A...

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Main Authors:	Cecilia Cristea, Robert Sandulescu, Anca Florea, Mihaela Tertis
Format:	Article
Language:	English
Published:	MDPI AG 2013-04-01
Series:	Sensors
Subjects:	horseradish peroxidase single and multi-wall carbon nanotubes carbon based electrodes conducting polymer nanocomposites acetaminophen
Online Access:	http://www.mdpi.com/1424-8220/13/4/4841

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spelling	doaj-b1e5f1671c9a4ad7a3734b8e4aa9bfa32020-11-25T01:41:36ZengMDPI AGSensors1424-82202013-04-011344841485410.3390/s130404841Carbon Based Electrodes Modified with Horseradish Peroxidase Immobilized in Conducting Polymers for Acetaminophen AnalysisCecilia CristeaRobert SandulescuAnca FloreaMihaela TertisThe development and optimization of new biosensors with horseradish peroxidase immobilized in carbon nanotubes-polyethyleneimine or polypyrrole nanocomposite film at the surface of two types of transducer is described. The amperometric detection of acetaminophen was carried out at −0.2 V versus Ag/AgCl using carbon based-screen printed electrodes (SPEs) and glassy carbon electrodes (GCEs) as transducers. The electroanalytical parameters of the biosensors are highly dependent on their configuration and on the dimensions of the carbon nanotubes. The best limit of detection obtained for acetaminophen was 1.36 ± 0.013 μM and the linear range 9.99–79.01 μM for the HRP-SWCNT/PEI in GCE configuration. The biosensors were successfully applied for the detection of acetaminophen in several drug formulations.http://www.mdpi.com/1424-8220/13/4/4841horseradish peroxidasesingle and multi-wall carbon nanotubescarbon based electrodesconducting polymernanocompositesacetaminophen
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Cecilia Cristea Robert Sandulescu Anca Florea Mihaela Tertis
spellingShingle	Cecilia Cristea Robert Sandulescu Anca Florea Mihaela Tertis Carbon Based Electrodes Modified with Horseradish Peroxidase Immobilized in Conducting Polymers for Acetaminophen Analysis Sensors horseradish peroxidase single and multi-wall carbon nanotubes carbon based electrodes conducting polymer nanocomposites acetaminophen
author_facet	Cecilia Cristea Robert Sandulescu Anca Florea Mihaela Tertis
author_sort	Cecilia Cristea
title	Carbon Based Electrodes Modified with Horseradish Peroxidase Immobilized in Conducting Polymers for Acetaminophen Analysis
title_short	Carbon Based Electrodes Modified with Horseradish Peroxidase Immobilized in Conducting Polymers for Acetaminophen Analysis
title_full	Carbon Based Electrodes Modified with Horseradish Peroxidase Immobilized in Conducting Polymers for Acetaminophen Analysis
title_fullStr	Carbon Based Electrodes Modified with Horseradish Peroxidase Immobilized in Conducting Polymers for Acetaminophen Analysis
title_full_unstemmed	Carbon Based Electrodes Modified with Horseradish Peroxidase Immobilized in Conducting Polymers for Acetaminophen Analysis
title_sort	carbon based electrodes modified with horseradish peroxidase immobilized in conducting polymers for acetaminophen analysis
publisher	MDPI AG
series	Sensors
issn	1424-8220
publishDate	2013-04-01
description	The development and optimization of new biosensors with horseradish peroxidase immobilized in carbon nanotubes-polyethyleneimine or polypyrrole nanocomposite film at the surface of two types of transducer is described. The amperometric detection of acetaminophen was carried out at −0.2 V versus Ag/AgCl using carbon based-screen printed electrodes (SPEs) and glassy carbon electrodes (GCEs) as transducers. The electroanalytical parameters of the biosensors are highly dependent on their configuration and on the dimensions of the carbon nanotubes. The best limit of detection obtained for acetaminophen was 1.36 ± 0.013 μM and the linear range 9.99–79.01 μM for the HRP-SWCNT/PEI in GCE configuration. The biosensors were successfully applied for the detection of acetaminophen in several drug formulations.
topic	horseradish peroxidase single and multi-wall carbon nanotubes carbon based electrodes conducting polymer nanocomposites acetaminophen
url	http://www.mdpi.com/1424-8220/13/4/4841
work_keys_str_mv	AT ceciliacristea carbonbasedelectrodesmodifiedwithhorseradishperoxidaseimmobilizedinconductingpolymersforacetaminophenanalysis AT robertsandulescu carbonbasedelectrodesmodifiedwithhorseradishperoxidaseimmobilizedinconductingpolymersforacetaminophenanalysis AT ancaflorea carbonbasedelectrodesmodifiedwithhorseradishperoxidaseimmobilizedinconductingpolymersforacetaminophenanalysis AT mihaelatertis carbonbasedelectrodesmodifiedwithhorseradishperoxidaseimmobilizedinconductingpolymersforacetaminophenanalysis
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Carbon Based Electrodes Modified with Horseradish Peroxidase Immobilized in Conducting Polymers for Acetaminophen Analysis

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