Possible involvement of Opa-interacting protein 5 in adipose proliferation and obesity.
Obesity is an epidemic matter increasing risk for cardiovascular diseases and metabolic disorders such as type 2 diabetes. We recently examined the association between visceral fat adiposity and gene expression profile of peripheral blood cells in human subjects. In a series of studies, Opa (Neisser...
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doaj-b1ed7ee981f14df4ab429dfee2bb43e32020-11-25T01:19:09ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0192e8766110.1371/journal.pone.0087661Possible involvement of Opa-interacting protein 5 in adipose proliferation and obesity.Kana InoueNorikazu MaedaTakuya MoriRyohei SekimotoYu TsushimaKeisuke MatsudaMasaya YamaokaTakayoshi SuganamiHitoshi NishizawaYoshihiro OgawaTohru FunahashiIichiro ShimomuraObesity is an epidemic matter increasing risk for cardiovascular diseases and metabolic disorders such as type 2 diabetes. We recently examined the association between visceral fat adiposity and gene expression profile of peripheral blood cells in human subjects. In a series of studies, Opa (Neisseria gonorrhoeae opacity-associated)-interacting protein 5 (OIP5) was nominated as a molecule of unknown function in adipocytes and thus the present study was performed to investigate the role of OIP5 in obesity. Adenovirus overexpressing Oip5 (Ad-Oip5) was generated and infected to 3T3-L1 cells stably expressing Coxsackie-Adenovirus Receptor (CAR-3T3-L1) and to mouse subcutaneous fat. For a knockdown experiment, siRNA against Oip5 (Oip5-siRNA) was introduced into 3T3-L1 cells. Proliferation of adipose cells was measured by BrdU uptake, EdU-staining, and cell count. Significant increase of Oip5 mRNA level was observed in obese white adipose tissues and such increase was detected in both mature adipocytes fraction and stromal vascular cell fraction. Ad-Oip5-infected CAR-3T3-L1 preadipocytes and adipocytes proliferated rapidly, while a significant reduction of proliferation was observed in Oip5-siRNA-introduced 3T3-L1 preadipocytes. Fat weight and number of adipocytes were significantly increased in Ad-Oip5-administered fat tissues. Oip5 promotes proliferation of pre- and mature-adipocytes and contributes adipose hyperplasia. Increase of Oip5 may associate with development of obesity.http://europepmc.org/articles/PMC3916335?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kana Inoue Norikazu Maeda Takuya Mori Ryohei Sekimoto Yu Tsushima Keisuke Matsuda Masaya Yamaoka Takayoshi Suganami Hitoshi Nishizawa Yoshihiro Ogawa Tohru Funahashi Iichiro Shimomura |
spellingShingle |
Kana Inoue Norikazu Maeda Takuya Mori Ryohei Sekimoto Yu Tsushima Keisuke Matsuda Masaya Yamaoka Takayoshi Suganami Hitoshi Nishizawa Yoshihiro Ogawa Tohru Funahashi Iichiro Shimomura Possible involvement of Opa-interacting protein 5 in adipose proliferation and obesity. PLoS ONE |
author_facet |
Kana Inoue Norikazu Maeda Takuya Mori Ryohei Sekimoto Yu Tsushima Keisuke Matsuda Masaya Yamaoka Takayoshi Suganami Hitoshi Nishizawa Yoshihiro Ogawa Tohru Funahashi Iichiro Shimomura |
author_sort |
Kana Inoue |
title |
Possible involvement of Opa-interacting protein 5 in adipose proliferation and obesity. |
title_short |
Possible involvement of Opa-interacting protein 5 in adipose proliferation and obesity. |
title_full |
Possible involvement of Opa-interacting protein 5 in adipose proliferation and obesity. |
title_fullStr |
Possible involvement of Opa-interacting protein 5 in adipose proliferation and obesity. |
title_full_unstemmed |
Possible involvement of Opa-interacting protein 5 in adipose proliferation and obesity. |
title_sort |
possible involvement of opa-interacting protein 5 in adipose proliferation and obesity. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
Obesity is an epidemic matter increasing risk for cardiovascular diseases and metabolic disorders such as type 2 diabetes. We recently examined the association between visceral fat adiposity and gene expression profile of peripheral blood cells in human subjects. In a series of studies, Opa (Neisseria gonorrhoeae opacity-associated)-interacting protein 5 (OIP5) was nominated as a molecule of unknown function in adipocytes and thus the present study was performed to investigate the role of OIP5 in obesity. Adenovirus overexpressing Oip5 (Ad-Oip5) was generated and infected to 3T3-L1 cells stably expressing Coxsackie-Adenovirus Receptor (CAR-3T3-L1) and to mouse subcutaneous fat. For a knockdown experiment, siRNA against Oip5 (Oip5-siRNA) was introduced into 3T3-L1 cells. Proliferation of adipose cells was measured by BrdU uptake, EdU-staining, and cell count. Significant increase of Oip5 mRNA level was observed in obese white adipose tissues and such increase was detected in both mature adipocytes fraction and stromal vascular cell fraction. Ad-Oip5-infected CAR-3T3-L1 preadipocytes and adipocytes proliferated rapidly, while a significant reduction of proliferation was observed in Oip5-siRNA-introduced 3T3-L1 preadipocytes. Fat weight and number of adipocytes were significantly increased in Ad-Oip5-administered fat tissues. Oip5 promotes proliferation of pre- and mature-adipocytes and contributes adipose hyperplasia. Increase of Oip5 may associate with development of obesity. |
url |
http://europepmc.org/articles/PMC3916335?pdf=render |
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