Enrichment and verification of differentially expressed miRNAs in bursa of Fabricius in two breeds of duck
Objective The bursa of Fabricius (BF) is a central humoral immune organ belonging specifically to avians. Recent studies had suggested that miRNAs were active regulators involved in the immune processes. This study was to investigate the possible differences of the BF at miRNA level between two gene...
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Asian-Australasian Association of Animal Production Societies
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doaj-b1ee6dd399834d3fafc152dedcf9d3262020-11-24T22:13:24ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172017-07-0130792092910.5713/ajas.16.032523634Enrichment and verification of differentially expressed miRNAs in bursa of Fabricius in two breeds of duckJun Luo0Junying Liu1Hehe Liu2Tao Zhang3Jiwen Wang4Hua He5Chunchun Han6 Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Wenjiang, Chengdu 611130, ChinaObjective The bursa of Fabricius (BF) is a central humoral immune organ belonging specifically to avians. Recent studies had suggested that miRNAs were active regulators involved in the immune processes. This study was to investigate the possible differences of the BF at miRNA level between two genetically disparate duck breeds. Methods Using Illumina next-generation sequencing, the miRNAs libraries of ducks were established. Results The results showed that there were 66 differentially expressed miRNAs and 28 novel miRNAs in bursa. A set of abundant miRNAs (i.e., let-7, miR-146a-5p, miR-21-5p, miR-17~92) which are involved in immunity and disease were detected and the predicted target genes of the novel miRNAs were associated with duck high anti-adversity ability. By gene ontology analysis and enriching KEGG pathway, the targets of differential expressed miRNAs were mainly involved in immunity and disease, supporting that there were differences in the BF immune functions between the two duck breeds. In addition, the metabolic pathway had the maximum enriched target genes and some enriched pathways that were related to cell cycle, protein synthesis, cell proliferation and apoptosis. It indicted that the difference of metabolism may be one of the reasons leading the immune difference between the BF of two duck breeds. Conclusion This data lists the main differences in the BF at miRNAs level between two genetically disparate duck breeds and lays a foundation to carry out molecular assisted breeding of poultry in the future.http://www.ajas.info/upload/pdf/ajas-30-7-920.pdfBursa of FabriciusMiRNAsGenetically Disparate BreedsGene OntologyKEGGDuck |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jun Luo Junying Liu Hehe Liu Tao Zhang Jiwen Wang Hua He Chunchun Han |
spellingShingle |
Jun Luo Junying Liu Hehe Liu Tao Zhang Jiwen Wang Hua He Chunchun Han Enrichment and verification of differentially expressed miRNAs in bursa of Fabricius in two breeds of duck Asian-Australasian Journal of Animal Sciences Bursa of Fabricius MiRNAs Genetically Disparate Breeds Gene Ontology KEGG Duck |
author_facet |
Jun Luo Junying Liu Hehe Liu Tao Zhang Jiwen Wang Hua He Chunchun Han |
author_sort |
Jun Luo |
title |
Enrichment and verification of differentially expressed miRNAs in bursa of Fabricius in two breeds of duck |
title_short |
Enrichment and verification of differentially expressed miRNAs in bursa of Fabricius in two breeds of duck |
title_full |
Enrichment and verification of differentially expressed miRNAs in bursa of Fabricius in two breeds of duck |
title_fullStr |
Enrichment and verification of differentially expressed miRNAs in bursa of Fabricius in two breeds of duck |
title_full_unstemmed |
Enrichment and verification of differentially expressed miRNAs in bursa of Fabricius in two breeds of duck |
title_sort |
enrichment and verification of differentially expressed mirnas in bursa of fabricius in two breeds of duck |
publisher |
Asian-Australasian Association of Animal Production Societies |
series |
Asian-Australasian Journal of Animal Sciences |
issn |
1011-2367 1976-5517 |
publishDate |
2017-07-01 |
description |
Objective The bursa of Fabricius (BF) is a central humoral immune organ belonging specifically to avians. Recent studies had suggested that miRNAs were active regulators involved in the immune processes. This study was to investigate the possible differences of the BF at miRNA level between two genetically disparate duck breeds. Methods Using Illumina next-generation sequencing, the miRNAs libraries of ducks were established. Results The results showed that there were 66 differentially expressed miRNAs and 28 novel miRNAs in bursa. A set of abundant miRNAs (i.e., let-7, miR-146a-5p, miR-21-5p, miR-17~92) which are involved in immunity and disease were detected and the predicted target genes of the novel miRNAs were associated with duck high anti-adversity ability. By gene ontology analysis and enriching KEGG pathway, the targets of differential expressed miRNAs were mainly involved in immunity and disease, supporting that there were differences in the BF immune functions between the two duck breeds. In addition, the metabolic pathway had the maximum enriched target genes and some enriched pathways that were related to cell cycle, protein synthesis, cell proliferation and apoptosis. It indicted that the difference of metabolism may be one of the reasons leading the immune difference between the BF of two duck breeds. Conclusion This data lists the main differences in the BF at miRNAs level between two genetically disparate duck breeds and lays a foundation to carry out molecular assisted breeding of poultry in the future. |
topic |
Bursa of Fabricius MiRNAs Genetically Disparate Breeds Gene Ontology KEGG Duck |
url |
http://www.ajas.info/upload/pdf/ajas-30-7-920.pdf |
work_keys_str_mv |
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