Semi‐automated biobank sample processing with a 384 high density sample tube robot used in cancer and cardiovascular studies
Abstract BackgroundIn the postgenomic era, it has become evident that analysis of genetic and protein expression changes alone is not sufficient to understand most disease processes in e.g. cardiovascular and cancer disease. Biobanking has been identified as an important area for development and dis...
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doaj-b203d0e8ac2843089886f92950ba72d12020-11-25T03:24:04ZengWileyClinical and Translational Medicine2001-13262015-12-0141n/an/a10.1186/s40169-015-0067-0Semi‐automated biobank sample processing with a 384 high density sample tube robot used in cancer and cardiovascular studiesJohan Malm0Henrik Lindberg1David Erlinge2Roger Appelqvist3Maria Yakovleva4Charlotte Welinder5Erik Steinfelder6Thomas E Fehniger7György Marko‐Varga8Section for Clinical ChemistryDepartment of Translational MedicineLund UniversitySkåne University Hospital Malmö205 02MalmöSwedenDepartment of Biomedical Engineering, Clinical Protein Science and ImagingBiomedical CenterLund University221 00LundSwedenDepartment of CardiologyLund UniversitySkåne University Hospital Lund221 85LundSwedenDepartment of Biomedical Engineering, Clinical Protein Science and ImagingBiomedical CenterLund University221 00LundSwedenCentre of Excellence in Biological and Medical Mass SpectrometryBiomedical Centre D13Lund University221 85LundSwedenCentre of Excellence in Biological and Medical Mass SpectrometryBiomedical Centre D13Lund University221 85LundSwedenThermoFisher ScientificGlasgowUKCentre of Excellence in Biological and Medical Mass SpectrometryBiomedical Centre D13Lund University221 85LundSwedenCentre of Excellence in Biological and Medical Mass SpectrometryBiomedical Centre D13Lund University221 85LundSwedenAbstract BackgroundIn the postgenomic era, it has become evident that analysis of genetic and protein expression changes alone is not sufficient to understand most disease processes in e.g. cardiovascular and cancer disease. Biobanking has been identified as an important area for development and discovery of better diagnostic tools and new treatment modalities. Biobanks are developed in order to integrate the collection of clinical samples from both healthy individuals and patients and provide valuable information that will make possible improved patient care. Modern healthcare developments are intimately linked to information based on studies of patient samples from biobank archives in large scale studies. Today biobanks form important national, as well as international, networks that share and combine global resources. MethodsWe have developed and validated a novel biobanking workflow process that utilizes 384‐tube systems with a high speed sample array robot with unique processing principles. ResultsThe 384‐tube format and robotic processing is incorporated into a cancer and cardiovascular diagnostic/prognostic research program with therapeutic interventions. Our biobank practice has gained acceptance within many hospitals and research units and is based on high‐density sample storage with small aliquot sample volumes. The previous standard of 5–10 mL sample volume tubes is being replaced by smaller volumes of 50–70 μL blood fractions that typically result in hundreds of thousands of aliquot fractions in 384‐tube systems. ConclusionsOur novel biobanking workflow process is robust and well suited for clinical studies.https://doi.org/10.1186/s40169-015-0067-0Biobank384 sample tubesCancerCardiovascular |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Johan Malm Henrik Lindberg David Erlinge Roger Appelqvist Maria Yakovleva Charlotte Welinder Erik Steinfelder Thomas E Fehniger György Marko‐Varga |
spellingShingle |
Johan Malm Henrik Lindberg David Erlinge Roger Appelqvist Maria Yakovleva Charlotte Welinder Erik Steinfelder Thomas E Fehniger György Marko‐Varga Semi‐automated biobank sample processing with a 384 high density sample tube robot used in cancer and cardiovascular studies Clinical and Translational Medicine Biobank 384 sample tubes Cancer Cardiovascular |
author_facet |
Johan Malm Henrik Lindberg David Erlinge Roger Appelqvist Maria Yakovleva Charlotte Welinder Erik Steinfelder Thomas E Fehniger György Marko‐Varga |
author_sort |
Johan Malm |
title |
Semi‐automated biobank sample processing with a 384 high density sample tube robot used in cancer and cardiovascular studies |
title_short |
Semi‐automated biobank sample processing with a 384 high density sample tube robot used in cancer and cardiovascular studies |
title_full |
Semi‐automated biobank sample processing with a 384 high density sample tube robot used in cancer and cardiovascular studies |
title_fullStr |
Semi‐automated biobank sample processing with a 384 high density sample tube robot used in cancer and cardiovascular studies |
title_full_unstemmed |
Semi‐automated biobank sample processing with a 384 high density sample tube robot used in cancer and cardiovascular studies |
title_sort |
semi‐automated biobank sample processing with a 384 high density sample tube robot used in cancer and cardiovascular studies |
publisher |
Wiley |
series |
Clinical and Translational Medicine |
issn |
2001-1326 |
publishDate |
2015-12-01 |
description |
Abstract BackgroundIn the postgenomic era, it has become evident that analysis of genetic and protein expression changes alone is not sufficient to understand most disease processes in e.g. cardiovascular and cancer disease. Biobanking has been identified as an important area for development and discovery of better diagnostic tools and new treatment modalities. Biobanks are developed in order to integrate the collection of clinical samples from both healthy individuals and patients and provide valuable information that will make possible improved patient care. Modern healthcare developments are intimately linked to information based on studies of patient samples from biobank archives in large scale studies. Today biobanks form important national, as well as international, networks that share and combine global resources. MethodsWe have developed and validated a novel biobanking workflow process that utilizes 384‐tube systems with a high speed sample array robot with unique processing principles. ResultsThe 384‐tube format and robotic processing is incorporated into a cancer and cardiovascular diagnostic/prognostic research program with therapeutic interventions. Our biobank practice has gained acceptance within many hospitals and research units and is based on high‐density sample storage with small aliquot sample volumes. The previous standard of 5–10 mL sample volume tubes is being replaced by smaller volumes of 50–70 μL blood fractions that typically result in hundreds of thousands of aliquot fractions in 384‐tube systems. ConclusionsOur novel biobanking workflow process is robust and well suited for clinical studies. |
topic |
Biobank 384 sample tubes Cancer Cardiovascular |
url |
https://doi.org/10.1186/s40169-015-0067-0 |
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