Fluorescence Detection of Deoxyadenosine in <i>Cordyceps</i> spp. by Indicator Displacement Assay
A rapid, sensitive and reliable indicator displacement assay (IDA) for specific detection of 2′- and 3′-deoxyadenosine (2′-dAde and 3′-dAde), the latter is also known as cordycepin, was established. The formation of inclusion complex between protonated acridine orange (AOH<sup>+</sup>) a...
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doaj-b2163f3251ce4885b82c4e8deb07d69d2020-11-25T02:41:31ZengMDPI AGMolecules1420-30492020-04-01252045204510.3390/molecules25092045Fluorescence Detection of Deoxyadenosine in <i>Cordyceps</i> spp. by Indicator Displacement AssayArinta Agnie Dewantari0Nattha Yongwattana1Panwajee Payongsri2Sawinee Seemakhan3Suparerk Borwornpinyo4Akio Ojida5Jirarut Wongkongkatep6Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, ThailandDepartment of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, ThailandDepartment of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, ThailandExcellent Center for Drug Discovery, Faculty of Science, Mahidol University, Bangkok 10400, ThailandDepartment of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, ThailandGraduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, JapanDepartment of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, ThailandA rapid, sensitive and reliable indicator displacement assay (IDA) for specific detection of 2′- and 3′-deoxyadenosine (2′-dAde and 3′-dAde), the latter is also known as cordycepin, was established. The formation of inclusion complex between protonated acridine orange (AOH<sup>+</sup>) and cucurbit[7]uril (CB7) resulted in the hypochromic shift of fluorescent emission from 530 nm to 512 nm. Addition of cordycepin to the highly fluorescent AOH<sup>+</sup>/CB7 complex resulted in a unique tripartite AOH<sup>+</sup>/CB7/dAde complex with diminished fluorescence, and such reduction in emission intensity serves as the basis for our novel sensing system. The detection limits were 11 and 82 μM for 2′- and 3′-deoxyadenosine, respectively. The proposed method also demonstrated high selectivity toward 2′- and 3′-deoxyadenosine, owing to the inability of other deoxynucleosides, nucleosides and nucleotides commonly found in <i>Cordyceps</i> spp. to displace the AOH<sup>+</sup> from the AOH<sup>+</sup>/CB7 complex, which was confirmed by isothermal titration calorimetry (ITC), UV-Visible and proton nuclear magnetic resonance (<sup>1</sup>H-NMR) spectroscopy. Our method was successfully implemented in the analysis of cordycepin in commercially available <i>Ophiocordyceps</i> and <i>Cordyceps</i> supplements, providing a novel and effective tool for quality assessment of these precious fungi with several health benefits.https://www.mdpi.com/1420-3049/25/9/2045cordycepinfungiindicator displacement assayfluorescencedetectioncucurbit uril |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Arinta Agnie Dewantari Nattha Yongwattana Panwajee Payongsri Sawinee Seemakhan Suparerk Borwornpinyo Akio Ojida Jirarut Wongkongkatep |
spellingShingle |
Arinta Agnie Dewantari Nattha Yongwattana Panwajee Payongsri Sawinee Seemakhan Suparerk Borwornpinyo Akio Ojida Jirarut Wongkongkatep Fluorescence Detection of Deoxyadenosine in <i>Cordyceps</i> spp. by Indicator Displacement Assay Molecules cordycepin fungi indicator displacement assay fluorescence detection cucurbit uril |
author_facet |
Arinta Agnie Dewantari Nattha Yongwattana Panwajee Payongsri Sawinee Seemakhan Suparerk Borwornpinyo Akio Ojida Jirarut Wongkongkatep |
author_sort |
Arinta Agnie Dewantari |
title |
Fluorescence Detection of Deoxyadenosine in <i>Cordyceps</i> spp. by Indicator Displacement Assay |
title_short |
Fluorescence Detection of Deoxyadenosine in <i>Cordyceps</i> spp. by Indicator Displacement Assay |
title_full |
Fluorescence Detection of Deoxyadenosine in <i>Cordyceps</i> spp. by Indicator Displacement Assay |
title_fullStr |
Fluorescence Detection of Deoxyadenosine in <i>Cordyceps</i> spp. by Indicator Displacement Assay |
title_full_unstemmed |
Fluorescence Detection of Deoxyadenosine in <i>Cordyceps</i> spp. by Indicator Displacement Assay |
title_sort |
fluorescence detection of deoxyadenosine in <i>cordyceps</i> spp. by indicator displacement assay |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2020-04-01 |
description |
A rapid, sensitive and reliable indicator displacement assay (IDA) for specific detection of 2′- and 3′-deoxyadenosine (2′-dAde and 3′-dAde), the latter is also known as cordycepin, was established. The formation of inclusion complex between protonated acridine orange (AOH<sup>+</sup>) and cucurbit[7]uril (CB7) resulted in the hypochromic shift of fluorescent emission from 530 nm to 512 nm. Addition of cordycepin to the highly fluorescent AOH<sup>+</sup>/CB7 complex resulted in a unique tripartite AOH<sup>+</sup>/CB7/dAde complex with diminished fluorescence, and such reduction in emission intensity serves as the basis for our novel sensing system. The detection limits were 11 and 82 μM for 2′- and 3′-deoxyadenosine, respectively. The proposed method also demonstrated high selectivity toward 2′- and 3′-deoxyadenosine, owing to the inability of other deoxynucleosides, nucleosides and nucleotides commonly found in <i>Cordyceps</i> spp. to displace the AOH<sup>+</sup> from the AOH<sup>+</sup>/CB7 complex, which was confirmed by isothermal titration calorimetry (ITC), UV-Visible and proton nuclear magnetic resonance (<sup>1</sup>H-NMR) spectroscopy. Our method was successfully implemented in the analysis of cordycepin in commercially available <i>Ophiocordyceps</i> and <i>Cordyceps</i> supplements, providing a novel and effective tool for quality assessment of these precious fungi with several health benefits. |
topic |
cordycepin fungi indicator displacement assay fluorescence detection cucurbit uril |
url |
https://www.mdpi.com/1420-3049/25/9/2045 |
work_keys_str_mv |
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