Cloning and biochemical characterization of a novel lipolytic gene from activated sludge metagenome, and its gene product
<p>Abstract</p> <p>In this study, a putative esterase, designated EstMY, was isolated from an activated sludge metagenomic library. The lipolytic gene was subcloned and expressed in <it>Escherichia coli </it>BL21 using the pET expression system. The gene <it>estMY...
Main Authors: | WenJun Han, KeGui Zhang, JunGang Li |
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Format: | Article |
Language: | English |
Published: |
BMC
2010-11-01
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Series: | Microbial Cell Factories |
Online Access: | http://www.microbialcellfactories.com/content/9/1/83 |
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