Autoimmune and non-autoimmune thyroid diseases have different patterns of cellular HLA class II expression
CONTEXT: Surface HLA-DR antigen is usually only expressed by antigen-presenting cells (APC). In autoimmune thyroid disease, follicle cells function as APC, thus expressing HLA-DR. However, non-autoimmune thyroid diseases may also express surface class II antigens. OBJECTIVE: To evaluate the presence...
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doaj-b2382d2a4d554e3db27bbaabcf7a3a6f2020-11-24T23:27:25ZengAssociação Paulista de MedicinaSão Paulo Medical Journal1806-9460117416116410.1590/S1516-31801999000400004S1516-31801999000400004Autoimmune and non-autoimmune thyroid diseases have different patterns of cellular HLA class II expressionDenise Engelbrecht Zantut-Wittmann0Luís Henrique Barbosa BoechatGlauce Aparecida Pinto1Miriam Aparecida da Silva Trevisan2José Vassallo3State University of CampinasState University of CampinasState University of CampinasState University of CampinasCONTEXT: Surface HLA-DR antigen is usually only expressed by antigen-presenting cells (APC). In autoimmune thyroid disease, follicle cells function as APC, thus expressing HLA-DR. However, non-autoimmune thyroid diseases may also express surface class II antigens. OBJECTIVE: To evaluate the presence and pattern of HLA class II expression in autoimmune and non-autoimmune thyroid disorders. DESIGN: Retrospective: histopathological and immunohistochemical analysis. LOCATION: Referral center, university hospital. SAMPLE: Ten histologically normal thyroids, 11 Graves’ disease, 7 Hashimoto’s thyroiditis, 10 atoxic multinodular goiter and 3 toxic adenomas were analyzed by immunohistochemistry, using a monoclonal antibody anti-HLA-DR. MAIN MEASUREMENTS: The presence of these antigens in thyroid follicular cells and their relation to inflammatory infiltrate was evaluated. The pattern of HLA-DR expression in thyroid follicular cells was analyzed: membrane, cytoplasmic or both. RESULTS: Although HLA-DR antigens were sparsely present in one of the 8 normal thyroids, in 6 of the 9 atoxic multinodular goiter and in 2 of the 3 toxic adenomas a net positivity could be seen in large areas. In all 5 Hashimoto’s thyroiditis and in 7 of the 10 Graves’ disease cases. This expression occurred in follicle cells either in contact with inflammatory cells or not. In non-autoimmune thyroid disease, HLA-DR positivity was essentially cytoplasmic, whereas in Graves’ disease and Hashimoto thyroiditis it was mainly in cell membranes. CONCLUSIONS: It is suggested that the HLA class II expression on the surface of follicle cells could be related to auto-antigen presentation to the immune system by these cells, leading to inflammation.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31801999000400004&lng=en&tlng=enGraves’ diseaseAutoimmune thyroiditisHLA-DR antigensImmunohistochemistry |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Denise Engelbrecht Zantut-Wittmann Luís Henrique Barbosa Boechat Glauce Aparecida Pinto Miriam Aparecida da Silva Trevisan José Vassallo |
spellingShingle |
Denise Engelbrecht Zantut-Wittmann Luís Henrique Barbosa Boechat Glauce Aparecida Pinto Miriam Aparecida da Silva Trevisan José Vassallo Autoimmune and non-autoimmune thyroid diseases have different patterns of cellular HLA class II expression São Paulo Medical Journal Graves’ disease Autoimmune thyroiditis HLA-DR antigens Immunohistochemistry |
author_facet |
Denise Engelbrecht Zantut-Wittmann Luís Henrique Barbosa Boechat Glauce Aparecida Pinto Miriam Aparecida da Silva Trevisan José Vassallo |
author_sort |
Denise Engelbrecht Zantut-Wittmann |
title |
Autoimmune and non-autoimmune thyroid diseases have different patterns of cellular HLA class II expression |
title_short |
Autoimmune and non-autoimmune thyroid diseases have different patterns of cellular HLA class II expression |
title_full |
Autoimmune and non-autoimmune thyroid diseases have different patterns of cellular HLA class II expression |
title_fullStr |
Autoimmune and non-autoimmune thyroid diseases have different patterns of cellular HLA class II expression |
title_full_unstemmed |
Autoimmune and non-autoimmune thyroid diseases have different patterns of cellular HLA class II expression |
title_sort |
autoimmune and non-autoimmune thyroid diseases have different patterns of cellular hla class ii expression |
publisher |
Associação Paulista de Medicina |
series |
São Paulo Medical Journal |
issn |
1806-9460 |
description |
CONTEXT: Surface HLA-DR antigen is usually only expressed by antigen-presenting cells (APC). In autoimmune thyroid disease, follicle cells function as APC, thus expressing HLA-DR. However, non-autoimmune thyroid diseases may also express surface class II antigens. OBJECTIVE: To evaluate the presence and pattern of HLA class II expression in autoimmune and non-autoimmune thyroid disorders. DESIGN: Retrospective: histopathological and immunohistochemical analysis. LOCATION: Referral center, university hospital. SAMPLE: Ten histologically normal thyroids, 11 Graves’ disease, 7 Hashimoto’s thyroiditis, 10 atoxic multinodular goiter and 3 toxic adenomas were analyzed by immunohistochemistry, using a monoclonal antibody anti-HLA-DR. MAIN MEASUREMENTS: The presence of these antigens in thyroid follicular cells and their relation to inflammatory infiltrate was evaluated. The pattern of HLA-DR expression in thyroid follicular cells was analyzed: membrane, cytoplasmic or both. RESULTS: Although HLA-DR antigens were sparsely present in one of the 8 normal thyroids, in 6 of the 9 atoxic multinodular goiter and in 2 of the 3 toxic adenomas a net positivity could be seen in large areas. In all 5 Hashimoto’s thyroiditis and in 7 of the 10 Graves’ disease cases. This expression occurred in follicle cells either in contact with inflammatory cells or not. In non-autoimmune thyroid disease, HLA-DR positivity was essentially cytoplasmic, whereas in Graves’ disease and Hashimoto thyroiditis it was mainly in cell membranes. CONCLUSIONS: It is suggested that the HLA class II expression on the surface of follicle cells could be related to auto-antigen presentation to the immune system by these cells, leading to inflammation. |
topic |
Graves’ disease Autoimmune thyroiditis HLA-DR antigens Immunohistochemistry |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31801999000400004&lng=en&tlng=en |
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