New MADS-box gene in fern: cloning and expression analysis of DfMADS1 from Dryopteris fragrans.
MADS genes encode a family of transcription factors, some of which control the identities of floral organs in flowering plants. Most of the MADS-box genes in fern have been cloned and analyzed in model plants, such as Ceratopteris richardii and Ceratopteris pteridoides. In this study, a new MADS-box...
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doaj-b23fa9fb43ce4518b2de2780e8383a732020-11-25T00:47:15ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0191e8634910.1371/journal.pone.0086349New MADS-box gene in fern: cloning and expression analysis of DfMADS1 from Dryopteris fragrans.Qingyang HuangWenhua LiRuifeng FanYing ChangMADS genes encode a family of transcription factors, some of which control the identities of floral organs in flowering plants. Most of the MADS-box genes in fern have been cloned and analyzed in model plants, such as Ceratopteris richardii and Ceratopteris pteridoides. In this study, a new MADS-box gene, DfMADS1(GU385475), was cloned from Dryopteris fragrans (L.) Schott to better understand the role of MADS genes in the evolution of floral organs. The full-length DfMADS1 cDNA was 973 bp in length with a 75 bp 5'-UTR and a 169 bp 3'-UTR. The DfMADS1 protein was predicted to contain a typical MIKC-type domain structure consisting of a MADS domain, a short I region, a K domain, and a C-terminal region. The DfMADS1 protein showed high homology with MADS box proteins from other ferns. Phylogenetic analysis revealed that DfMADS1 belongs to the CRM1-like subfamily. RT-PCR analysis indicated that DfMADS1 is expressed in both the gametophytes and the sporophytes of D. fragrans.http://europepmc.org/articles/PMC3899247?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qingyang Huang Wenhua Li Ruifeng Fan Ying Chang |
spellingShingle |
Qingyang Huang Wenhua Li Ruifeng Fan Ying Chang New MADS-box gene in fern: cloning and expression analysis of DfMADS1 from Dryopteris fragrans. PLoS ONE |
author_facet |
Qingyang Huang Wenhua Li Ruifeng Fan Ying Chang |
author_sort |
Qingyang Huang |
title |
New MADS-box gene in fern: cloning and expression analysis of DfMADS1 from Dryopteris fragrans. |
title_short |
New MADS-box gene in fern: cloning and expression analysis of DfMADS1 from Dryopteris fragrans. |
title_full |
New MADS-box gene in fern: cloning and expression analysis of DfMADS1 from Dryopteris fragrans. |
title_fullStr |
New MADS-box gene in fern: cloning and expression analysis of DfMADS1 from Dryopteris fragrans. |
title_full_unstemmed |
New MADS-box gene in fern: cloning and expression analysis of DfMADS1 from Dryopteris fragrans. |
title_sort |
new mads-box gene in fern: cloning and expression analysis of dfmads1 from dryopteris fragrans. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
MADS genes encode a family of transcription factors, some of which control the identities of floral organs in flowering plants. Most of the MADS-box genes in fern have been cloned and analyzed in model plants, such as Ceratopteris richardii and Ceratopteris pteridoides. In this study, a new MADS-box gene, DfMADS1(GU385475), was cloned from Dryopteris fragrans (L.) Schott to better understand the role of MADS genes in the evolution of floral organs. The full-length DfMADS1 cDNA was 973 bp in length with a 75 bp 5'-UTR and a 169 bp 3'-UTR. The DfMADS1 protein was predicted to contain a typical MIKC-type domain structure consisting of a MADS domain, a short I region, a K domain, and a C-terminal region. The DfMADS1 protein showed high homology with MADS box proteins from other ferns. Phylogenetic analysis revealed that DfMADS1 belongs to the CRM1-like subfamily. RT-PCR analysis indicated that DfMADS1 is expressed in both the gametophytes and the sporophytes of D. fragrans. |
url |
http://europepmc.org/articles/PMC3899247?pdf=render |
work_keys_str_mv |
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