Identification and characterization of a glyoxalase I gene in a rapeseed cultivar with seed thermotolerance
Glyoxalase I (GLYI) is a ubiquitous enzyme in all organisms that catalyzes the conversion of the potent cytotoxin methylglyoxal to S-D-lactoylglutathione. Although many reports suggest the importance of GLYI in the plant response to stress, its function in seeds requires further study. Here, we iden...
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doaj-b2423b18f965433f94b7574dd3a682a42020-11-24T21:30:08ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-02-01710.3389/fpls.2016.00150169306Identification and characterization of a glyoxalase I gene in a rapeseed cultivar with seed thermotoleranceGuixin eYan0Xiaodan eLv1Guizhen eGao2Feng eLi3Jun eLi4Jiangwei eQiao5Kun eXu6Biyun eChen7Limin eWang8Xin eXiao9Xiaoming eWu10Oil Crops Research Institute, Chinese Academy of Agricultural SciencesOil Crops Research Institute, Chinese Academy of Agricultural SciencesOil Crops Research Institute, Chinese Academy of Agricultural SciencesOil Crops Research Institute, Chinese Academy of Agricultural SciencesOil Crops Research Institute, Chinese Academy of Agricultural SciencesOil Crops Research Institute, Chinese Academy of Agricultural SciencesOil Crops Research Institute, Chinese Academy of Agricultural SciencesOil Crops Research Institute, Chinese Academy of Agricultural SciencesOil Crops Research Institute, Chinese Academy of Agricultural SciencesOil Crops Research Institute, Chinese Academy of Agricultural SciencesOil Crops Research Institute, Chinese Academy of Agricultural SciencesGlyoxalase I (GLYI) is a ubiquitous enzyme in all organisms that catalyzes the conversion of the potent cytotoxin methylglyoxal to S-D-lactoylglutathione. Although many reports suggest the importance of GLYI in the plant response to stress, its function in seeds requires further study. Here, we identified a heat-induced GLYI from Brassica napus seeds, BnGLYI, using a comparative proteomics approach. Two-dimensional gel analyses revealed that BnGLYI protein expression upon heat treatment was significantly elevated in thermotolerant seeds but was diminished in heat-sensitive seeds. The BnGLYI-2 and BnGLYI-3 genes from the heat-sensitive and thermotolerant cultivars, respectively, were characterized and analyzed. Only two amino acid residue variations were found between the amino acid sequences of the two genes. Moreover, overexpressing BnGLYI-3 in yeast cells enhanced tolerance to heat and cold stress and significantly increased GLYI activity compared to overexpressing BnGLYI-2. In addition, BnGLYI-3 transformants showed enhanced superoxide dismutase activities under heat and cold treatment, whereas these activities were diminished for BnGLYI-2 transformants. Taken together, these results indicate that overexpression of the BnGLYI-3 gene imparts thermotolerance and cold tolerance in yeast and that the variations in BnGLYI-3 may play an important role in the responses to temperature stresses.http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00150/fullProteomicsyeastBrassica napus L.Glyoxalase I geneSeed thermotolerance |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Guixin eYan Xiaodan eLv Guizhen eGao Feng eLi Jun eLi Jiangwei eQiao Kun eXu Biyun eChen Limin eWang Xin eXiao Xiaoming eWu |
spellingShingle |
Guixin eYan Xiaodan eLv Guizhen eGao Feng eLi Jun eLi Jiangwei eQiao Kun eXu Biyun eChen Limin eWang Xin eXiao Xiaoming eWu Identification and characterization of a glyoxalase I gene in a rapeseed cultivar with seed thermotolerance Frontiers in Plant Science Proteomics yeast Brassica napus L. Glyoxalase I gene Seed thermotolerance |
author_facet |
Guixin eYan Xiaodan eLv Guizhen eGao Feng eLi Jun eLi Jiangwei eQiao Kun eXu Biyun eChen Limin eWang Xin eXiao Xiaoming eWu |
author_sort |
Guixin eYan |
title |
Identification and characterization of a glyoxalase I gene in a rapeseed cultivar with seed thermotolerance |
title_short |
Identification and characterization of a glyoxalase I gene in a rapeseed cultivar with seed thermotolerance |
title_full |
Identification and characterization of a glyoxalase I gene in a rapeseed cultivar with seed thermotolerance |
title_fullStr |
Identification and characterization of a glyoxalase I gene in a rapeseed cultivar with seed thermotolerance |
title_full_unstemmed |
Identification and characterization of a glyoxalase I gene in a rapeseed cultivar with seed thermotolerance |
title_sort |
identification and characterization of a glyoxalase i gene in a rapeseed cultivar with seed thermotolerance |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2016-02-01 |
description |
Glyoxalase I (GLYI) is a ubiquitous enzyme in all organisms that catalyzes the conversion of the potent cytotoxin methylglyoxal to S-D-lactoylglutathione. Although many reports suggest the importance of GLYI in the plant response to stress, its function in seeds requires further study. Here, we identified a heat-induced GLYI from Brassica napus seeds, BnGLYI, using a comparative proteomics approach. Two-dimensional gel analyses revealed that BnGLYI protein expression upon heat treatment was significantly elevated in thermotolerant seeds but was diminished in heat-sensitive seeds. The BnGLYI-2 and BnGLYI-3 genes from the heat-sensitive and thermotolerant cultivars, respectively, were characterized and analyzed. Only two amino acid residue variations were found between the amino acid sequences of the two genes. Moreover, overexpressing BnGLYI-3 in yeast cells enhanced tolerance to heat and cold stress and significantly increased GLYI activity compared to overexpressing BnGLYI-2. In addition, BnGLYI-3 transformants showed enhanced superoxide dismutase activities under heat and cold treatment, whereas these activities were diminished for BnGLYI-2 transformants. Taken together, these results indicate that overexpression of the BnGLYI-3 gene imparts thermotolerance and cold tolerance in yeast and that the variations in BnGLYI-3 may play an important role in the responses to temperature stresses. |
topic |
Proteomics yeast Brassica napus L. Glyoxalase I gene Seed thermotolerance |
url |
http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00150/full |
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