Prevalence of bee viruses in Apis cerana cerana populations from different locations in the Fujian Province of China

• Main Authors: Aqai Kalan Hassanyar, Shaokang Huang, Zhiguo Li, Muhammad Rizwan, Shahid Mehmood, Muhammad Fahad Raza, Muhammad Qasim, Mubasher Hussain, Songkun Su
• Format: Article
• Language: English
• Published: Wiley 2019-09-01
• Series: MicrobiologyOpen
• Subjects: Apis cerana cerana; Fujian; honeybee; prevalence; RT‐PCR; viruses
• Online Access: https://doi.org/10.1002/mbo3.830

Abstract Prevalence of honeybee viral diseases has recently been causing major problems in the beekeeping industry, causing economic losses worldwide. Honeybees are susceptible to a variety of diseases and various pathogens. Among these pathogens, prevalence viruses, along with other factors, are seriously threatening the health of bee species. In the present study, samples were collected from 80 Apis cerana cerana (A. c. cerana) colonies from three different locations, Cangshan, Fuan, and Yongtai, in the Fujian Province of China. All samples were screened using the reverse transcription polymerase chain reaction (RT‐PCR) method for detection of seven honeybee viruses, namely, Chinese sacbrood virus (CSBV), deformed wing virus (DWV), Israeli acute paralysis virus (IAPV), black queen cell virus (BQCV), chronic bee paralysis virus (CBPV), acute bee paralysis virus (ABPV), and Kashmir bee virus (KBV). Our results showed that CSBV was the most prevalent as it was detected in (90%), of the samples, DWV was detected in (81.25%), and IAPV was detected in (26.25%). In contrast, insignificant prevalence results were obtained from all apiaries for BQCV, CBPV, APBV, and KBV, which were not detected in any sample. Here, we are providing the first report on the molecular detection of honeybee viruses, especially the prevalence of IAPV, from different regions in the Fujian Province of China with a high prevalence of bee viruses, on A. c. cerana, and there is great concern for the presence of honeybee viruses in the population of the native honeybee (A. c. cerana) in China.