Mechanism of Intestinal Flora and Proteomics on Regulating Immune Function of Durio zibethinus Rind Polysaccharide
In this study, cyclophosphamide was injected intraperitoneally to establish an immunosuppressive mouse model to study the immune regulating effects of Durio zibethinus Murr rind polysaccharide (DZMP) through proteomics and intestinal flora. The results showed that the thymus and spleen indexes of th...
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Online Access: | http://dx.doi.org/10.1155/2021/6614028 |
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doaj-b254ca8040c04210b9475eca965275482021-02-15T12:53:11ZengHindawi LimitedOxidative Medicine and Cellular Longevity1942-09001942-09942021-01-01202110.1155/2021/66140286614028Mechanism of Intestinal Flora and Proteomics on Regulating Immune Function of Durio zibethinus Rind PolysaccharideHuimin Jiang0Jinmei Wang1Qiongxin Liang2Shengjun Jiang3Changyang Ma4Yan Zhang5Wenyi Kang6National R&D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, 475004 Henan Province, ChinaNational R&D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, 475004 Henan Province, ChinaNational R&D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, 475004 Henan Province, ChinaNational R&D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, 475004 Henan Province, ChinaNational R&D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, 475004 Henan Province, ChinaCollege of Forensic Medicine, Hebei Medical University, Shijiazhuang 050017, ChinaNational R&D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, 475004 Henan Province, ChinaIn this study, cyclophosphamide was injected intraperitoneally to establish an immunosuppressive mouse model to study the immune regulating effects of Durio zibethinus Murr rind polysaccharide (DZMP) through proteomics and intestinal flora. The results showed that the thymus and spleen indexes of the high-dose DZMP (200 mg/kg) group were significantly increased, and the tissue structure of the spleen was improved compared with the model group (P<0.01). The contents of IL-2, IL-4, IL-6, and TNF-α in the high-dose group of DZMP were significantly increased (P<0.001). Activities of acid phosphatase (ACP), lactate dehydrogenase (LDH), superoxide dismutase (SOD), and total antioxidant capacity (T-AOC) were increased in serum (P<0.01). In the liver, catalase (CAT) activity was increased (P<0.001) while the malondialdehyde (MDA) content was decreased and immune activity was increased (P<0.001). Proteomics studies showed that the drug group could significantly increase the low-affinity immunoglobulin gamma Fc receptor III (FcγRIII) protein and protein kinase C-α (PKC-α) compared with the model group (P<0.001). In addition, the result showed that those proteins were likely involved in the regulation of the metabolic pathways of autoimmune thyroid disease, Staphylococcus aureus infection, and NF-κB signaling pathway. Intestinal microbial studies showed that short-chain fatty acid (SCFA) content was increased as well as the relative abundance of beneficial bacteria Akkermansia, Bacteroides, and Paraprevotella, while the relative abundance of Ruminococcus and Oscillospira was decreased compared with the model group (P<0.001). The results showed that DZMP might play a beneficial role in immune regulation by improving intestinal flora.http://dx.doi.org/10.1155/2021/6614028 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Huimin Jiang Jinmei Wang Qiongxin Liang Shengjun Jiang Changyang Ma Yan Zhang Wenyi Kang |
spellingShingle |
Huimin Jiang Jinmei Wang Qiongxin Liang Shengjun Jiang Changyang Ma Yan Zhang Wenyi Kang Mechanism of Intestinal Flora and Proteomics on Regulating Immune Function of Durio zibethinus Rind Polysaccharide Oxidative Medicine and Cellular Longevity |
author_facet |
Huimin Jiang Jinmei Wang Qiongxin Liang Shengjun Jiang Changyang Ma Yan Zhang Wenyi Kang |
author_sort |
Huimin Jiang |
title |
Mechanism of Intestinal Flora and Proteomics on Regulating Immune Function of Durio zibethinus Rind Polysaccharide |
title_short |
Mechanism of Intestinal Flora and Proteomics on Regulating Immune Function of Durio zibethinus Rind Polysaccharide |
title_full |
Mechanism of Intestinal Flora and Proteomics on Regulating Immune Function of Durio zibethinus Rind Polysaccharide |
title_fullStr |
Mechanism of Intestinal Flora and Proteomics on Regulating Immune Function of Durio zibethinus Rind Polysaccharide |
title_full_unstemmed |
Mechanism of Intestinal Flora and Proteomics on Regulating Immune Function of Durio zibethinus Rind Polysaccharide |
title_sort |
mechanism of intestinal flora and proteomics on regulating immune function of durio zibethinus rind polysaccharide |
publisher |
Hindawi Limited |
series |
Oxidative Medicine and Cellular Longevity |
issn |
1942-0900 1942-0994 |
publishDate |
2021-01-01 |
description |
In this study, cyclophosphamide was injected intraperitoneally to establish an immunosuppressive mouse model to study the immune regulating effects of Durio zibethinus Murr rind polysaccharide (DZMP) through proteomics and intestinal flora. The results showed that the thymus and spleen indexes of the high-dose DZMP (200 mg/kg) group were significantly increased, and the tissue structure of the spleen was improved compared with the model group (P<0.01). The contents of IL-2, IL-4, IL-6, and TNF-α in the high-dose group of DZMP were significantly increased (P<0.001). Activities of acid phosphatase (ACP), lactate dehydrogenase (LDH), superoxide dismutase (SOD), and total antioxidant capacity (T-AOC) were increased in serum (P<0.01). In the liver, catalase (CAT) activity was increased (P<0.001) while the malondialdehyde (MDA) content was decreased and immune activity was increased (P<0.001). Proteomics studies showed that the drug group could significantly increase the low-affinity immunoglobulin gamma Fc receptor III (FcγRIII) protein and protein kinase C-α (PKC-α) compared with the model group (P<0.001). In addition, the result showed that those proteins were likely involved in the regulation of the metabolic pathways of autoimmune thyroid disease, Staphylococcus aureus infection, and NF-κB signaling pathway. Intestinal microbial studies showed that short-chain fatty acid (SCFA) content was increased as well as the relative abundance of beneficial bacteria Akkermansia, Bacteroides, and Paraprevotella, while the relative abundance of Ruminococcus and Oscillospira was decreased compared with the model group (P<0.001). The results showed that DZMP might play a beneficial role in immune regulation by improving intestinal flora. |
url |
http://dx.doi.org/10.1155/2021/6614028 |
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