Biomimetic Mineralization on a Macroporous Cellulose-Based Matrix for Bone Regeneration
The aim of this study is to investigate the biomimetic mineralization on a cellulose-based porous matrix with an improved biological profile. The cellulose matrix was precalcified using three methods: (i) cellulose samples were treated with a solution of calcium chloride and diammonium hydrogen phos...
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Online Access: | http://dx.doi.org/10.1155/2013/452750 |
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doaj-b26dd72ed13d4f6a85a2be2fe603dbf12020-11-24T22:43:12ZengHindawi LimitedBioMed Research International2314-61332314-61412013-01-01201310.1155/2013/452750452750Biomimetic Mineralization on a Macroporous Cellulose-Based Matrix for Bone RegenerationOdeta Petrauskaite0Pedro de Sousa Gomes1Maria Helena Fernandes2Gintaras Juodzbalys3Arturas Stumbras4Julius Maminskas5Jolanta Liesiene6Marco Cicciù7Department of Organic Technology, Kaunas University of Technology, Radvilenu pl. 19, 50254 Kaunas, LithuaniaLaboratory for Bone Metabolism and Regeneration, Faculty of Dental Medicine, University of Porto, Rua Dr. Manuel Pereira da Silva, 4200-392 Porto, PortugalLaboratory for Bone Metabolism and Regeneration, Faculty of Dental Medicine, University of Porto, Rua Dr. Manuel Pereira da Silva, 4200-392 Porto, PortugalDepartment of Oral and Maxillofacial Surgery, Lithuanian University of Health Sciences, Eiveniu str. 2, 50009 Kaunas, LithuaniaDepartment of Oral and Maxillofacial Surgery, Lithuanian University of Health Sciences, Eiveniu str. 2, 50009 Kaunas, LithuaniaDepartment of Oral and Maxillofacial Surgery, Lithuanian University of Health Sciences, Eiveniu str. 2, 50009 Kaunas, LithuaniaDepartment of Organic Technology, Kaunas University of Technology, Radvilenu pl. 19, 50254 Kaunas, LithuaniaHuman Pathology Department, Dental School, University of Messina, Messina IT, Policlinico G. Martino, Via Consolare Valeria, 98100 Messina, ItalyThe aim of this study is to investigate the biomimetic mineralization on a cellulose-based porous matrix with an improved biological profile. The cellulose matrix was precalcified using three methods: (i) cellulose samples were treated with a solution of calcium chloride and diammonium hydrogen phosphate; (ii) the carboxymethylated cellulose matrix was stored in a saturated calcium hydroxide solution; (iii) the cellulose matrix was mixed with a calcium silicate solution in order to introduce silanol groups and to combine them with calcium ions. All the methods resulted in a mineralization of the cellulose surfaces after immersion in a simulated body fluid solution. Over a period of 14 days, the matrix was completely covered with hydroxyapatite crystals. Hydroxyapatite formation depended on functional groups on the matrix surface as well as on the precalcification method. The largest hydroxyapatite crystals were obtained on the carboxymethylated cellulose matrix treated with calcium hydroxide solution. The porous cellulose matrix was not cytotoxic, allowing the adhesion and proliferation of human osteoblastic cells. Comparatively, improved cell adhesion and growth rate were achieved on the mineralized cellulose matrices.http://dx.doi.org/10.1155/2013/452750 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Odeta Petrauskaite Pedro de Sousa Gomes Maria Helena Fernandes Gintaras Juodzbalys Arturas Stumbras Julius Maminskas Jolanta Liesiene Marco Cicciù |
spellingShingle |
Odeta Petrauskaite Pedro de Sousa Gomes Maria Helena Fernandes Gintaras Juodzbalys Arturas Stumbras Julius Maminskas Jolanta Liesiene Marco Cicciù Biomimetic Mineralization on a Macroporous Cellulose-Based Matrix for Bone Regeneration BioMed Research International |
author_facet |
Odeta Petrauskaite Pedro de Sousa Gomes Maria Helena Fernandes Gintaras Juodzbalys Arturas Stumbras Julius Maminskas Jolanta Liesiene Marco Cicciù |
author_sort |
Odeta Petrauskaite |
title |
Biomimetic Mineralization on a Macroporous Cellulose-Based Matrix for Bone Regeneration |
title_short |
Biomimetic Mineralization on a Macroporous Cellulose-Based Matrix for Bone Regeneration |
title_full |
Biomimetic Mineralization on a Macroporous Cellulose-Based Matrix for Bone Regeneration |
title_fullStr |
Biomimetic Mineralization on a Macroporous Cellulose-Based Matrix for Bone Regeneration |
title_full_unstemmed |
Biomimetic Mineralization on a Macroporous Cellulose-Based Matrix for Bone Regeneration |
title_sort |
biomimetic mineralization on a macroporous cellulose-based matrix for bone regeneration |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2013-01-01 |
description |
The aim of this study is to investigate the biomimetic mineralization on a cellulose-based porous matrix with an improved biological profile. The cellulose matrix was precalcified using three methods: (i) cellulose samples were treated with a solution of calcium chloride and diammonium hydrogen phosphate; (ii) the carboxymethylated cellulose matrix was stored in a saturated calcium hydroxide solution; (iii) the cellulose matrix was mixed with a calcium silicate solution in order to introduce silanol groups and to combine them with calcium ions. All the methods resulted in a mineralization of the cellulose surfaces after immersion in a simulated body fluid solution. Over a period of 14 days, the matrix was completely covered with hydroxyapatite crystals. Hydroxyapatite formation depended on functional groups on the matrix surface as well as on the precalcification method. The largest hydroxyapatite crystals were obtained on the carboxymethylated cellulose matrix treated with calcium hydroxide solution. The porous cellulose matrix was not cytotoxic, allowing the adhesion and proliferation of human osteoblastic cells. Comparatively, improved cell adhesion and growth rate were achieved on the mineralized cellulose matrices. |
url |
http://dx.doi.org/10.1155/2013/452750 |
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