6-Methoxymellein Isolated from Carrot (<i>Daucus carota </i>L.) Targets Breast Cancer Stem Cells by Regulating NF-κB Signaling
The presence of breast cancer stem cells (BCSCs) induces the aggressive progression and recurrence of breast cancer. These cells are drug resistant, have the capacity to self-renew and differentiate and are involved in recurrence and metastasis, suggesting that targeting BCSCs may improve treatment...
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doaj-b2839e785f5a4b8d83d2ab56e6d5b5042020-11-25T02:51:49ZengMDPI AGMolecules1420-30492020-09-01254374437410.3390/molecules251943746-Methoxymellein Isolated from Carrot (<i>Daucus carota </i>L.) Targets Breast Cancer Stem Cells by Regulating NF-κB SignalingRen Liu0Hack Sun Choi1Su-Lim Kim2Ji-Hyang Kim3Bong-Sik Yun4Dong-Sun Lee5Interdisciplinary Graduate Program in Advanced Convergence Technology and Science, Jeju National University, Jeju 63243, KoreaSubtropical/Tropical Organism Gene Bank, Jeju National University, Jeju 63243, KoreaInterdisciplinary Graduate Program in Advanced Convergence Technology and Science, Jeju National University, Jeju 63243, KoreaInterdisciplinary Graduate Program in Advanced Convergence Technology and Science, Jeju National University, Jeju 63243, KoreaDivision of Biotechnology, College of Environmental and Bioresource Sciences, Jeonbuk National University, Gobong-ro 79, Iksan 54596, KoreaInterdisciplinary Graduate Program in Advanced Convergence Technology and Science, Jeju National University, Jeju 63243, KoreaThe presence of breast cancer stem cells (BCSCs) induces the aggressive progression and recurrence of breast cancer. These cells are drug resistant, have the capacity to self-renew and differentiate and are involved in recurrence and metastasis, suggesting that targeting BCSCs may improve treatment efficacy. In this report, methanol extracts of carrot root were purified by means of silica gel, Sephadex LH-20, and preparative high-performance liquid chromatography to isolate a compound targeting mammosphere formation. We isolated the compound 6-methoxymellein, which inhibits the proliferation and migration of breast cancer cells, reduces mammosphere growth, decreases the proportion of CD44<sup>+</sup>/CD24<sup>−</sup> cells in breast cancer cells and decreases the expression of stemness-associated proteins c-Myc, Sox-2 and Oct4. 6-Methoxymellein reduces the nuclear localization of nuclear factor-κB (NF-κB) subunit p65 and p50. Subsequently, 6-methoxymellein decreases the mRNA transcription and secretion of IL-6 and IL-8. Our data suggest that 6-methoxymellein may be an anticancer agent that inhibits BCSCs via NF-κB/IL-6 and IL-8 regulation.https://www.mdpi.com/1420-3049/25/19/4374breast cancer stem cells (BCSCs)6-methoxymelleinNF-κB p65NF-κB p50IL-6IL-8 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ren Liu Hack Sun Choi Su-Lim Kim Ji-Hyang Kim Bong-Sik Yun Dong-Sun Lee |
spellingShingle |
Ren Liu Hack Sun Choi Su-Lim Kim Ji-Hyang Kim Bong-Sik Yun Dong-Sun Lee 6-Methoxymellein Isolated from Carrot (<i>Daucus carota </i>L.) Targets Breast Cancer Stem Cells by Regulating NF-κB Signaling Molecules breast cancer stem cells (BCSCs) 6-methoxymellein NF-κB p65 NF-κB p50 IL-6 IL-8 |
author_facet |
Ren Liu Hack Sun Choi Su-Lim Kim Ji-Hyang Kim Bong-Sik Yun Dong-Sun Lee |
author_sort |
Ren Liu |
title |
6-Methoxymellein Isolated from Carrot (<i>Daucus carota </i>L.) Targets Breast Cancer Stem Cells by Regulating NF-κB Signaling |
title_short |
6-Methoxymellein Isolated from Carrot (<i>Daucus carota </i>L.) Targets Breast Cancer Stem Cells by Regulating NF-κB Signaling |
title_full |
6-Methoxymellein Isolated from Carrot (<i>Daucus carota </i>L.) Targets Breast Cancer Stem Cells by Regulating NF-κB Signaling |
title_fullStr |
6-Methoxymellein Isolated from Carrot (<i>Daucus carota </i>L.) Targets Breast Cancer Stem Cells by Regulating NF-κB Signaling |
title_full_unstemmed |
6-Methoxymellein Isolated from Carrot (<i>Daucus carota </i>L.) Targets Breast Cancer Stem Cells by Regulating NF-κB Signaling |
title_sort |
6-methoxymellein isolated from carrot (<i>daucus carota </i>l.) targets breast cancer stem cells by regulating nf-κb signaling |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2020-09-01 |
description |
The presence of breast cancer stem cells (BCSCs) induces the aggressive progression and recurrence of breast cancer. These cells are drug resistant, have the capacity to self-renew and differentiate and are involved in recurrence and metastasis, suggesting that targeting BCSCs may improve treatment efficacy. In this report, methanol extracts of carrot root were purified by means of silica gel, Sephadex LH-20, and preparative high-performance liquid chromatography to isolate a compound targeting mammosphere formation. We isolated the compound 6-methoxymellein, which inhibits the proliferation and migration of breast cancer cells, reduces mammosphere growth, decreases the proportion of CD44<sup>+</sup>/CD24<sup>−</sup> cells in breast cancer cells and decreases the expression of stemness-associated proteins c-Myc, Sox-2 and Oct4. 6-Methoxymellein reduces the nuclear localization of nuclear factor-κB (NF-κB) subunit p65 and p50. Subsequently, 6-methoxymellein decreases the mRNA transcription and secretion of IL-6 and IL-8. Our data suggest that 6-methoxymellein may be an anticancer agent that inhibits BCSCs via NF-κB/IL-6 and IL-8 regulation. |
topic |
breast cancer stem cells (BCSCs) 6-methoxymellein NF-κB p65 NF-κB p50 IL-6 IL-8 |
url |
https://www.mdpi.com/1420-3049/25/19/4374 |
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