In Vivo Expression of Interleukin-37 Reduces Local and Systemic Inflammation in Concanavalin A-Induced Hepatitis
We recently reported that after LPS stimulation, IL-37 translocates to the nucleus and reduces the expression of proinflammatory cytokines. The aim of this study was to investigate whether transiently expressed IL-37 in mice reduces inflammation in concanavalin A (ConA)-induced hepatitis and LPS-ind...
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doaj-b2855ad716004702a81885e9693359fb2020-11-25T00:12:43ZengHindawi LimitedThe Scientific World Journal1537-744X2011-01-01112480249010.1100/2011/968479968479In Vivo Expression of Interleukin-37 Reduces Local and Systemic Inflammation in Concanavalin A-Induced HepatitisAna-Maria Bulau0Michaela Fink1Christof Maucksch2Roland Kappler3Doris Mayr4Kai Wagner5Philip Bufler6Department of Pediatrics, Dr. von Hauner Children’s Hospital, Ludwig-Maximilians-University, 80337 Munich, GermanyDepartment of Pediatrics, Dr. von Hauner Children’s Hospital, Ludwig-Maximilians-University, 80337 Munich, GermanyDepartment of Pediatrics, Dr. von Hauner Children’s Hospital, Ludwig-Maximilians-University, 80337 Munich, GermanyDepartment of Pediatric Surgery, Dr. von Hauner Children’s Hospital, Ludwig-Maximilians-University, 80337 Munich, GermanyInstitute of Pathology, Ludwig-Maximilians-University, 80337 Munich, GermanyInstitute of Pathology, Ludwig-Maximilians-University, 80337 Munich, GermanyDepartment of Pediatrics, Dr. von Hauner Children’s Hospital, Ludwig-Maximilians-University, 80337 Munich, GermanyWe recently reported that after LPS stimulation, IL-37 translocates to the nucleus and reduces the expression of proinflammatory cytokines. The aim of this study was to investigate whether transiently expressed IL-37 in mice reduces inflammation in concanavalin A (ConA)-induced hepatitis and LPS-induced sepsis. Transgene IL-37 expression was detected in the liver lysate of mice injected with IL-37 plasmid-DNA after hydrodynamic tail vein injection. All mice developed severe acute hepatitis after ConA injection. No difference in the histological score and serum ALT was observed between the two groups that might be explained by patchy expression of IL-37 protein in the liver. However, 2 hrs after ConA injection, serum levels for IL-1α, IL-6, IL-5, and IL-9 were significantly reduced in IL-37-expressing mice as seen for the LPS model. In conclusion, in vivo expression of human IL-37 in mice reduces local and systemic inflammation in ConA-induced hepatitis and LPS challenge.http://dx.doi.org/10.1100/2011/968479 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ana-Maria Bulau Michaela Fink Christof Maucksch Roland Kappler Doris Mayr Kai Wagner Philip Bufler |
spellingShingle |
Ana-Maria Bulau Michaela Fink Christof Maucksch Roland Kappler Doris Mayr Kai Wagner Philip Bufler In Vivo Expression of Interleukin-37 Reduces Local and Systemic Inflammation in Concanavalin A-Induced Hepatitis The Scientific World Journal |
author_facet |
Ana-Maria Bulau Michaela Fink Christof Maucksch Roland Kappler Doris Mayr Kai Wagner Philip Bufler |
author_sort |
Ana-Maria Bulau |
title |
In Vivo Expression of Interleukin-37 Reduces Local and Systemic Inflammation in Concanavalin A-Induced Hepatitis |
title_short |
In Vivo Expression of Interleukin-37 Reduces Local and Systemic Inflammation in Concanavalin A-Induced Hepatitis |
title_full |
In Vivo Expression of Interleukin-37 Reduces Local and Systemic Inflammation in Concanavalin A-Induced Hepatitis |
title_fullStr |
In Vivo Expression of Interleukin-37 Reduces Local and Systemic Inflammation in Concanavalin A-Induced Hepatitis |
title_full_unstemmed |
In Vivo Expression of Interleukin-37 Reduces Local and Systemic Inflammation in Concanavalin A-Induced Hepatitis |
title_sort |
in vivo expression of interleukin-37 reduces local and systemic inflammation in concanavalin a-induced hepatitis |
publisher |
Hindawi Limited |
series |
The Scientific World Journal |
issn |
1537-744X |
publishDate |
2011-01-01 |
description |
We recently reported that after LPS stimulation, IL-37 translocates to the nucleus and reduces the expression of proinflammatory cytokines. The aim of this study was to investigate whether transiently expressed IL-37 in mice reduces inflammation in concanavalin A (ConA)-induced hepatitis and LPS-induced sepsis. Transgene IL-37 expression was detected in the liver lysate of mice injected with IL-37 plasmid-DNA after hydrodynamic tail vein injection. All mice developed severe acute hepatitis after ConA injection. No difference in the histological score and serum ALT was observed between the two groups that might be explained by patchy expression of IL-37 protein in the liver. However, 2 hrs after ConA injection, serum levels for IL-1α, IL-6, IL-5, and IL-9 were significantly reduced in IL-37-expressing mice as seen for the LPS model. In conclusion, in vivo expression of human IL-37 in mice reduces local and systemic inflammation in ConA-induced hepatitis and LPS challenge. |
url |
http://dx.doi.org/10.1100/2011/968479 |
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