Enzymatic response of ryegrass cellulose and hemicellulose valorization introduced by sequential alkaline extractions
Abstract Background In view of the natural resistance of hemicelluloses in lignocellulosic biomass on bioconversion of cellulose into fermentable sugars, alkali extraction is considered as an effective method for gradually fractionating hemicelluloses and increasing the bioconversion efficiency of c...
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doaj-b29fb1e8736d44fc80be39632df8868e2021-03-21T12:50:13ZengBMCBiotechnology for Biofuels1754-68342021-03-0114111210.1186/s13068-021-01921-1Enzymatic response of ryegrass cellulose and hemicellulose valorization introduced by sequential alkaline extractionsShao-Fei Sun0Jing Yang1Da-Wei Wang2Hai-Yan Yang3Shao-Ni Sun4Zheng-Jun Shi5Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry UniversityKey Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry UniversityKey Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry UniversityKey Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry UniversityBeijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry UniversityKey Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry UniversityAbstract Background In view of the natural resistance of hemicelluloses in lignocellulosic biomass on bioconversion of cellulose into fermentable sugars, alkali extraction is considered as an effective method for gradually fractionating hemicelluloses and increasing the bioconversion efficiency of cellulose. In the present study, sequential alkaline extractions were performed on the delignified ryegrass material to achieve high bioconversion efficiency of cellulose and comprehensively investigated the structural features of hemicellulosic fractions for further applications. Results Sequential alkaline extractions removed hemicelluloses from cellulose-rich substrates and degraded part of amorphous cellulose, reducing yields of cellulose-rich substrates from 73.0 to 27.7% and increasing crystallinity indexes from 31.7 to 41.0%. Alkaline extraction enhanced bioconversion of cellulose by removal of hemicelluloses and swelling of cellulose, increasing of enzymatic hydrolysis from 72.3 to 95.3%. In addition, alkaline extraction gradually fractionated hemicelluloses into six fractions, containing arabinoxylans as the main polysaccharides and part of β-glucans. Simultaneously, increasing of alkaline concentration degraded hemicellulosic polysaccharides, which resulted in a decreasing their molecular weights from 67,510 to 50,720 g/mol. Conclusions The present study demonstrated that the sequential alkaline extraction conditions had significant effects on the enzymatic hydrolysis efficiency of cellulose and the investigation of the physicochemical properties of hemicellulose. Overall, the investigation the enzymatic hydrolysis efficiency of cellulose-rich substrates and the structural features of hemicelluloses from ryegrass will provide useful information for the efficient utilization of cellulose and hemicelluloses in biorefineries.https://doi.org/10.1186/s13068-021-01921-1RyegrassCelluloseHemicelluloses structureEnzymatic hydrolysisAlkaline extraction |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shao-Fei Sun Jing Yang Da-Wei Wang Hai-Yan Yang Shao-Ni Sun Zheng-Jun Shi |
spellingShingle |
Shao-Fei Sun Jing Yang Da-Wei Wang Hai-Yan Yang Shao-Ni Sun Zheng-Jun Shi Enzymatic response of ryegrass cellulose and hemicellulose valorization introduced by sequential alkaline extractions Biotechnology for Biofuels Ryegrass Cellulose Hemicelluloses structure Enzymatic hydrolysis Alkaline extraction |
author_facet |
Shao-Fei Sun Jing Yang Da-Wei Wang Hai-Yan Yang Shao-Ni Sun Zheng-Jun Shi |
author_sort |
Shao-Fei Sun |
title |
Enzymatic response of ryegrass cellulose and hemicellulose valorization introduced by sequential alkaline extractions |
title_short |
Enzymatic response of ryegrass cellulose and hemicellulose valorization introduced by sequential alkaline extractions |
title_full |
Enzymatic response of ryegrass cellulose and hemicellulose valorization introduced by sequential alkaline extractions |
title_fullStr |
Enzymatic response of ryegrass cellulose and hemicellulose valorization introduced by sequential alkaline extractions |
title_full_unstemmed |
Enzymatic response of ryegrass cellulose and hemicellulose valorization introduced by sequential alkaline extractions |
title_sort |
enzymatic response of ryegrass cellulose and hemicellulose valorization introduced by sequential alkaline extractions |
publisher |
BMC |
series |
Biotechnology for Biofuels |
issn |
1754-6834 |
publishDate |
2021-03-01 |
description |
Abstract Background In view of the natural resistance of hemicelluloses in lignocellulosic biomass on bioconversion of cellulose into fermentable sugars, alkali extraction is considered as an effective method for gradually fractionating hemicelluloses and increasing the bioconversion efficiency of cellulose. In the present study, sequential alkaline extractions were performed on the delignified ryegrass material to achieve high bioconversion efficiency of cellulose and comprehensively investigated the structural features of hemicellulosic fractions for further applications. Results Sequential alkaline extractions removed hemicelluloses from cellulose-rich substrates and degraded part of amorphous cellulose, reducing yields of cellulose-rich substrates from 73.0 to 27.7% and increasing crystallinity indexes from 31.7 to 41.0%. Alkaline extraction enhanced bioconversion of cellulose by removal of hemicelluloses and swelling of cellulose, increasing of enzymatic hydrolysis from 72.3 to 95.3%. In addition, alkaline extraction gradually fractionated hemicelluloses into six fractions, containing arabinoxylans as the main polysaccharides and part of β-glucans. Simultaneously, increasing of alkaline concentration degraded hemicellulosic polysaccharides, which resulted in a decreasing their molecular weights from 67,510 to 50,720 g/mol. Conclusions The present study demonstrated that the sequential alkaline extraction conditions had significant effects on the enzymatic hydrolysis efficiency of cellulose and the investigation of the physicochemical properties of hemicellulose. Overall, the investigation the enzymatic hydrolysis efficiency of cellulose-rich substrates and the structural features of hemicelluloses from ryegrass will provide useful information for the efficient utilization of cellulose and hemicelluloses in biorefineries. |
topic |
Ryegrass Cellulose Hemicelluloses structure Enzymatic hydrolysis Alkaline extraction |
url |
https://doi.org/10.1186/s13068-021-01921-1 |
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