Vaccination with the immunoglobulin M-degrading enzyme of Streptococcus suis, IdeSsuis, leads to protection against a highly virulent serotype 9 strain
Vaccination of weaning piglets with the recombinant IgM degrading enzyme of Streptococcus suis (S. suis), rIdeSsuis, elicits protection against disease caused by serotype (cps) 2 infection. In Europe, S. suis cps9 is at least as important as cps2 in causing severe herd problems associated with menin...
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doaj-b2b1026379bf4dc5aa45ff290a6dc78a2020-11-25T02:40:03ZengElsevierVaccine: X2590-13622019-12-013Vaccination with the immunoglobulin M-degrading enzyme of Streptococcus suis, IdeSsuis, leads to protection against a highly virulent serotype 9 strainKaroline Rieckmann0Anna Seydel1Kristin Klose2Gottfried Alber3Christoph G. Baums4Nicole Schütze5Institute of Bacteriology and Mycology, Centre for Infectious Diseases, Faculty of Veterinary Medicine, Leipzig University, Leipzig, GermanyInstitute of Bacteriology and Mycology, Centre for Infectious Diseases, Faculty of Veterinary Medicine, Leipzig University, Leipzig, GermanyInstitute of Pathology, Faculty of Veterinary Medicine, Leipzig University, Leipzig, GermanyInstitute of Immunology, Centre for Infectious Diseases, Faculty of Veterinary Medicine, Leipzig University, Leipzig, GermanyInstitute of Bacteriology and Mycology, Centre for Infectious Diseases, Faculty of Veterinary Medicine, Leipzig University, Leipzig, Germany; Corresponding author at: Universität Leipzig, Zentrum für Infektionsmedizin, Institut für Bakteriologie und Mykologie, An den Tierkliniken 29, 04103 Leipzig, Germany.Institute of Immunology, Centre for Infectious Diseases, Faculty of Veterinary Medicine, Leipzig University, Leipzig, GermanyVaccination of weaning piglets with the recombinant IgM degrading enzyme of Streptococcus suis (S. suis), rIdeSsuis, elicits protection against disease caused by serotype (cps) 2 infection. In Europe, S. suis cps9 is at least as important as cps2 in causing severe herd problems associated with meningitis, septicemia and arthritis. The objective of this study was to determine humoral and cellular immunogenicities of rIdeSsuis suckling piglet vaccination and to investigate protection against a virulent cps9 strain. Vaccination in the 2nd and 4th week of life with rIdeSsuis and an oil-in-water adjuvant induced seroconversion against IdeSsuis in 13 of 20 vaccinated piglets. In the 5th week, survival of the S. suis cps9 strain was significantly reduced in the blood of prime-booster vaccinated piglets. After a 2nd booster vaccination IdeSsuis-reactive T helper (Th) cells partially producing TNF-α, IL-17A or IFN-ɣ were detectable in rIdeSsuis-vaccinated but not in placebo-treated piglets and frequencies of IdeSsuis-reactive Th cells correlated with α-IdeSsuis–IgG levels. An intravenous challenge, conducted with a cps9 strain of sequence type (ST) 94, led to 89% mortality in placebo-treated piglets due to septicemia and meningitis. In contrast, all rIdeSsuis prime-booster-booster vaccinated littermates survived the challenge despite signs of disease such as fever and lameness. In conclusion, the described rIdeSsuis vaccination induces humoral and detectable IdeSsuis-reactive Th cell responses and leads to protection against a highly virulent cps9 strain. Keywords: IgM protease, T helper cell, Antigen-reactive, Pigs, Bactericidal assay, Serotype 9http://www.sciencedirect.com/science/article/pii/S2590136219300476 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Karoline Rieckmann Anna Seydel Kristin Klose Gottfried Alber Christoph G. Baums Nicole Schütze |
spellingShingle |
Karoline Rieckmann Anna Seydel Kristin Klose Gottfried Alber Christoph G. Baums Nicole Schütze Vaccination with the immunoglobulin M-degrading enzyme of Streptococcus suis, IdeSsuis, leads to protection against a highly virulent serotype 9 strain Vaccine: X |
author_facet |
Karoline Rieckmann Anna Seydel Kristin Klose Gottfried Alber Christoph G. Baums Nicole Schütze |
author_sort |
Karoline Rieckmann |
title |
Vaccination with the immunoglobulin M-degrading enzyme of Streptococcus suis, IdeSsuis, leads to protection against a highly virulent serotype 9 strain |
title_short |
Vaccination with the immunoglobulin M-degrading enzyme of Streptococcus suis, IdeSsuis, leads to protection against a highly virulent serotype 9 strain |
title_full |
Vaccination with the immunoglobulin M-degrading enzyme of Streptococcus suis, IdeSsuis, leads to protection against a highly virulent serotype 9 strain |
title_fullStr |
Vaccination with the immunoglobulin M-degrading enzyme of Streptococcus suis, IdeSsuis, leads to protection against a highly virulent serotype 9 strain |
title_full_unstemmed |
Vaccination with the immunoglobulin M-degrading enzyme of Streptococcus suis, IdeSsuis, leads to protection against a highly virulent serotype 9 strain |
title_sort |
vaccination with the immunoglobulin m-degrading enzyme of streptococcus suis, idessuis, leads to protection against a highly virulent serotype 9 strain |
publisher |
Elsevier |
series |
Vaccine: X |
issn |
2590-1362 |
publishDate |
2019-12-01 |
description |
Vaccination of weaning piglets with the recombinant IgM degrading enzyme of Streptococcus suis (S. suis), rIdeSsuis, elicits protection against disease caused by serotype (cps) 2 infection. In Europe, S. suis cps9 is at least as important as cps2 in causing severe herd problems associated with meningitis, septicemia and arthritis. The objective of this study was to determine humoral and cellular immunogenicities of rIdeSsuis suckling piglet vaccination and to investigate protection against a virulent cps9 strain. Vaccination in the 2nd and 4th week of life with rIdeSsuis and an oil-in-water adjuvant induced seroconversion against IdeSsuis in 13 of 20 vaccinated piglets. In the 5th week, survival of the S. suis cps9 strain was significantly reduced in the blood of prime-booster vaccinated piglets. After a 2nd booster vaccination IdeSsuis-reactive T helper (Th) cells partially producing TNF-α, IL-17A or IFN-ɣ were detectable in rIdeSsuis-vaccinated but not in placebo-treated piglets and frequencies of IdeSsuis-reactive Th cells correlated with α-IdeSsuis–IgG levels. An intravenous challenge, conducted with a cps9 strain of sequence type (ST) 94, led to 89% mortality in placebo-treated piglets due to septicemia and meningitis. In contrast, all rIdeSsuis prime-booster-booster vaccinated littermates survived the challenge despite signs of disease such as fever and lameness. In conclusion, the described rIdeSsuis vaccination induces humoral and detectable IdeSsuis-reactive Th cell responses and leads to protection against a highly virulent cps9 strain. Keywords: IgM protease, T helper cell, Antigen-reactive, Pigs, Bactericidal assay, Serotype 9 |
url |
http://www.sciencedirect.com/science/article/pii/S2590136219300476 |
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