MCA1 and MCA2 Are Involved in the Response to Hypergravity in Arabidopsis Hypocotyls
Plants respond to and resist gravitational acceleration, but the mechanism of signal perception in the response is unknown. We studied the role of MCA (mid1-complementing activity) proteins in gravity perception by analyzing the expression of the MCA1 and MCA2 genes, and the growth of hypocotyls of...
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doaj-b2bfc1bec6074eedb48539516f85b37c2020-11-25T02:11:54ZengMDPI AGPlants2223-77472020-05-01959059010.3390/plants9050590MCA1 and MCA2 Are Involved in the Response to Hypergravity in Arabidopsis HypocotylsTakayuki Hattori0Yasuhiro Otomi1Yohei Nakajima2Kouichi Soga3Kazuyuki Wakabayashi4Hidetoshi Iida5Takayuki Hoson6Department of Biology, Graduate School of Science, Osaka City University, Sumiyoshi-ku, Osaka 558-8585, JapanDepartment of Biology, Graduate School of Science, Osaka City University, Sumiyoshi-ku, Osaka 558-8585, JapanDepartment of Biology, Graduate School of Science, Osaka City University, Sumiyoshi-ku, Osaka 558-8585, JapanDepartment of Biology, Graduate School of Science, Osaka City University, Sumiyoshi-ku, Osaka 558-8585, JapanDepartment of Biology, Graduate School of Science, Osaka City University, Sumiyoshi-ku, Osaka 558-8585, JapanDepartment of Biology, Tokyo Gakugei University, Koganei, Tokyo 184-8501, JapanDepartment of Biology, Graduate School of Science, Osaka City University, Sumiyoshi-ku, Osaka 558-8585, JapanPlants respond to and resist gravitational acceleration, but the mechanism of signal perception in the response is unknown. We studied the role of MCA (mid1-complementing activity) proteins in gravity perception by analyzing the expression of the MCA1 and MCA2 genes, and the growth of hypocotyls of mca mutants, under hypergravity conditions in the dark. An MCA1 promoter::GUS fusion reporter gene construct (MCA1p::GUS) and MCA2p::GUS were expressed almost universally in etiolated seedlings. Under hypergravity conditions, the expression levels of both genes increased compared with that under the 1 g condition, and remained higher, especially in the basal supporting region. On the other hand, mca-null and MCA-overexpressing seedlings showed normal growth under the 1 g condition. Hypergravity suppressed elongation growth of hypocotyls, but this effect was reduced in hypocotyls of mca-null mutants compared with the wild type. In contrast, MCA-overexpressing seedlings were hypersensitive to increased gravity; suppression of elongation growth was detected at a lower gravity level than that in the wild type. These results suggest that MCAs are involved in the perception of gravity signals in plants, and may be responsible for resistance to hypergravity.https://www.mdpi.com/2223-7747/9/5/590Arabidopsisgravity resistancehypergravityMCA1MCA2mechanosensitive channel |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Takayuki Hattori Yasuhiro Otomi Yohei Nakajima Kouichi Soga Kazuyuki Wakabayashi Hidetoshi Iida Takayuki Hoson |
spellingShingle |
Takayuki Hattori Yasuhiro Otomi Yohei Nakajima Kouichi Soga Kazuyuki Wakabayashi Hidetoshi Iida Takayuki Hoson MCA1 and MCA2 Are Involved in the Response to Hypergravity in Arabidopsis Hypocotyls Plants Arabidopsis gravity resistance hypergravity MCA1 MCA2 mechanosensitive channel |
author_facet |
Takayuki Hattori Yasuhiro Otomi Yohei Nakajima Kouichi Soga Kazuyuki Wakabayashi Hidetoshi Iida Takayuki Hoson |
author_sort |
Takayuki Hattori |
title |
MCA1 and MCA2 Are Involved in the Response to Hypergravity in Arabidopsis Hypocotyls |
title_short |
MCA1 and MCA2 Are Involved in the Response to Hypergravity in Arabidopsis Hypocotyls |
title_full |
MCA1 and MCA2 Are Involved in the Response to Hypergravity in Arabidopsis Hypocotyls |
title_fullStr |
MCA1 and MCA2 Are Involved in the Response to Hypergravity in Arabidopsis Hypocotyls |
title_full_unstemmed |
MCA1 and MCA2 Are Involved in the Response to Hypergravity in Arabidopsis Hypocotyls |
title_sort |
mca1 and mca2 are involved in the response to hypergravity in arabidopsis hypocotyls |
publisher |
MDPI AG |
series |
Plants |
issn |
2223-7747 |
publishDate |
2020-05-01 |
description |
Plants respond to and resist gravitational acceleration, but the mechanism of signal perception in the response is unknown. We studied the role of MCA (mid1-complementing activity) proteins in gravity perception by analyzing the expression of the MCA1 and MCA2 genes, and the growth of hypocotyls of mca mutants, under hypergravity conditions in the dark. An MCA1 promoter::GUS fusion reporter gene construct (MCA1p::GUS) and MCA2p::GUS were expressed almost universally in etiolated seedlings. Under hypergravity conditions, the expression levels of both genes increased compared with that under the 1 g condition, and remained higher, especially in the basal supporting region. On the other hand, mca-null and MCA-overexpressing seedlings showed normal growth under the 1 g condition. Hypergravity suppressed elongation growth of hypocotyls, but this effect was reduced in hypocotyls of mca-null mutants compared with the wild type. In contrast, MCA-overexpressing seedlings were hypersensitive to increased gravity; suppression of elongation growth was detected at a lower gravity level than that in the wild type. These results suggest that MCAs are involved in the perception of gravity signals in plants, and may be responsible for resistance to hypergravity. |
topic |
Arabidopsis gravity resistance hypergravity MCA1 MCA2 mechanosensitive channel |
url |
https://www.mdpi.com/2223-7747/9/5/590 |
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