Regulatory effects of cAMP receptor protein (CRP) on porin genes and its own gene in <it>Yersinia pestis</it>
<p>Abstract</p> <p>Background</p> <p>The cAMP receptor protein (CRP) is a global bacterial regulator that controls many target genes. The CRP-cAMP complex regulates the <it>ompR-envZ </it>operon in <it>E. coli </it>directly, involving both positi...
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doaj-b2cb4f1c93304801990592bb573d1ca52020-11-24T20:51:35ZengBMCBMC Microbiology1471-21802011-02-011114010.1186/1471-2180-11-40Regulatory effects of cAMP receptor protein (CRP) on porin genes and its own gene in <it>Yersinia pestis</it>Han YanpingTan YafangGuo ZhaobiaoLiu XiaYang LinZhang YiquanGao HeHuang XinxiangZhou DongshengYang Ruifu<p>Abstract</p> <p>Background</p> <p>The cAMP receptor protein (CRP) is a global bacterial regulator that controls many target genes. The CRP-cAMP complex regulates the <it>ompR-envZ </it>operon in <it>E. coli </it>directly, involving both positive and negative regulations of multiple target promoters; further, it controls the production of porins indirectly through its direct action on <it>ompR-envZ</it>. Auto-regulation of CRP has also been established in <it>E. coli</it>. However, the regulation of porin genes and its own gene by CRP remains unclear in <it>Y. pestis</it>.</p> <p>Results</p> <p><it>Y. pestis </it>employs a distinct mechanism indicating that CRP has no regulatory effect on the <it>ompR-envZ </it>operon; however, it stimulates <it>ompC </it>and <it>ompF </it>directly, while repressing <it>ompX</it>. No transcriptional regulatory association between CRP and its own gene can be detected in <it>Y. pestis</it>, which is also in contrast to the fact that CRP acts as both repressor and activator for its own gene in <it>E. coli</it>. It is likely that <it>Y. pestis </it>OmpR and CRP respectively sense different signals (medium osmolarity, and cellular cAMP levels) to regulate porin genes independently.</p> <p>Conclusion</p> <p>Although the CRP of <it>Y. pestis </it>shows a very high homology to that of <it>E. coli</it>, and the consensus DNA sequence recognized by CRP is shared by the two bacteria, the <it>Y. pestis </it>CRP can recognize the promoters of <it>ompC</it>, <it>F</it>, and <it>X </it>directly rather than that of its own gene, which is different from the relevant regulatory circuit of <it>E. coli</it>. Data presented here indicate a remarkable remodeling of the CRP-mediated regulation of porin genes and of its own one between these two bacteria.</p> http://www.biomedcentral.com/1471-2180/11/40 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Han Yanping Tan Yafang Guo Zhaobiao Liu Xia Yang Lin Zhang Yiquan Gao He Huang Xinxiang Zhou Dongsheng Yang Ruifu |
spellingShingle |
Han Yanping Tan Yafang Guo Zhaobiao Liu Xia Yang Lin Zhang Yiquan Gao He Huang Xinxiang Zhou Dongsheng Yang Ruifu Regulatory effects of cAMP receptor protein (CRP) on porin genes and its own gene in <it>Yersinia pestis</it> BMC Microbiology |
author_facet |
Han Yanping Tan Yafang Guo Zhaobiao Liu Xia Yang Lin Zhang Yiquan Gao He Huang Xinxiang Zhou Dongsheng Yang Ruifu |
author_sort |
Han Yanping |
title |
Regulatory effects of cAMP receptor protein (CRP) on porin genes and its own gene in <it>Yersinia pestis</it> |
title_short |
Regulatory effects of cAMP receptor protein (CRP) on porin genes and its own gene in <it>Yersinia pestis</it> |
title_full |
Regulatory effects of cAMP receptor protein (CRP) on porin genes and its own gene in <it>Yersinia pestis</it> |
title_fullStr |
Regulatory effects of cAMP receptor protein (CRP) on porin genes and its own gene in <it>Yersinia pestis</it> |
title_full_unstemmed |
Regulatory effects of cAMP receptor protein (CRP) on porin genes and its own gene in <it>Yersinia pestis</it> |
title_sort |
regulatory effects of camp receptor protein (crp) on porin genes and its own gene in <it>yersinia pestis</it> |
publisher |
BMC |
series |
BMC Microbiology |
issn |
1471-2180 |
publishDate |
2011-02-01 |
description |
<p>Abstract</p> <p>Background</p> <p>The cAMP receptor protein (CRP) is a global bacterial regulator that controls many target genes. The CRP-cAMP complex regulates the <it>ompR-envZ </it>operon in <it>E. coli </it>directly, involving both positive and negative regulations of multiple target promoters; further, it controls the production of porins indirectly through its direct action on <it>ompR-envZ</it>. Auto-regulation of CRP has also been established in <it>E. coli</it>. However, the regulation of porin genes and its own gene by CRP remains unclear in <it>Y. pestis</it>.</p> <p>Results</p> <p><it>Y. pestis </it>employs a distinct mechanism indicating that CRP has no regulatory effect on the <it>ompR-envZ </it>operon; however, it stimulates <it>ompC </it>and <it>ompF </it>directly, while repressing <it>ompX</it>. No transcriptional regulatory association between CRP and its own gene can be detected in <it>Y. pestis</it>, which is also in contrast to the fact that CRP acts as both repressor and activator for its own gene in <it>E. coli</it>. It is likely that <it>Y. pestis </it>OmpR and CRP respectively sense different signals (medium osmolarity, and cellular cAMP levels) to regulate porin genes independently.</p> <p>Conclusion</p> <p>Although the CRP of <it>Y. pestis </it>shows a very high homology to that of <it>E. coli</it>, and the consensus DNA sequence recognized by CRP is shared by the two bacteria, the <it>Y. pestis </it>CRP can recognize the promoters of <it>ompC</it>, <it>F</it>, and <it>X </it>directly rather than that of its own gene, which is different from the relevant regulatory circuit of <it>E. coli</it>. Data presented here indicate a remarkable remodeling of the CRP-mediated regulation of porin genes and of its own one between these two bacteria.</p> |
url |
http://www.biomedcentral.com/1471-2180/11/40 |
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