Jianpi Huayu Decoction Inhibits Proliferation in Human Colorectal Cancer Cells (SW480) by Inducing G0/G1-Phase Cell Cycle Arrest and Apoptosis
Jianpi Huayu Decoction (JHD), a Chinese medicine formula, is a typical prescription against multiple tumors in the clinical treatment, which can raise quality of life and decrease complications. The aim of this study is to assess the efficacy of JHD against human colorectal carcinoma cells (SW480) a...
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doaj-b2eaf91adc904cf085aeb0a73f093dab2020-11-24T20:49:53ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-427X1741-42882015-01-01201510.1155/2015/236506236506Jianpi Huayu Decoction Inhibits Proliferation in Human Colorectal Cancer Cells (SW480) by Inducing G0/G1-Phase Cell Cycle Arrest and ApoptosisSong-yang Xi0Yu-hao Teng1Yan Chen2Jie-ping Li3Ying-ying Zhang4Shen-lin Liu5Xi Zou6Jin-yong Zhou7Jian Wu8Rui-ping Wang9The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, ChinaThe Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, ChinaThe Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, ChinaThe Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, ChinaThe Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, ChinaThe Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, ChinaThe Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, ChinaThe Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, ChinaThe Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, ChinaThe Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, ChinaJianpi Huayu Decoction (JHD), a Chinese medicine formula, is a typical prescription against multiple tumors in the clinical treatment, which can raise quality of life and decrease complications. The aim of this study is to assess the efficacy of JHD against human colorectal carcinoma cells (SW480) and explore its mechanism. MTT assay showed that JHD decreased the cellular viability of SW480 cells in dose-dependent and time-dependent manner. Flow cytometry analysis revealed that JHD induced G0/G1-phase cell cycle arrest in SW480 cells and had a strong apoptosis-inducing effect on SW480 cells. Meanwhile it enhanced the expression of p27, cleaved PARP, cleaved caspase-3, and Bax and decreased the levels of PARP, caspase-3, Bcl-2, CDK2, CDK4, CDK6, cyclin D1, cyclin D2, cyclin D3, and cyclin E1, which was evidenced by RT-qPCR and Western blot analysis. In conclusion, these results indicated that JHD inhibited proliferation in SW480 cells by inducing G0/G1-phase cell cycle arrest and apoptosis, providing a practicaltherapeutic strategy against colorectal cancer.http://dx.doi.org/10.1155/2015/236506 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Song-yang Xi Yu-hao Teng Yan Chen Jie-ping Li Ying-ying Zhang Shen-lin Liu Xi Zou Jin-yong Zhou Jian Wu Rui-ping Wang |
spellingShingle |
Song-yang Xi Yu-hao Teng Yan Chen Jie-ping Li Ying-ying Zhang Shen-lin Liu Xi Zou Jin-yong Zhou Jian Wu Rui-ping Wang Jianpi Huayu Decoction Inhibits Proliferation in Human Colorectal Cancer Cells (SW480) by Inducing G0/G1-Phase Cell Cycle Arrest and Apoptosis Evidence-Based Complementary and Alternative Medicine |
author_facet |
Song-yang Xi Yu-hao Teng Yan Chen Jie-ping Li Ying-ying Zhang Shen-lin Liu Xi Zou Jin-yong Zhou Jian Wu Rui-ping Wang |
author_sort |
Song-yang Xi |
title |
Jianpi Huayu Decoction Inhibits Proliferation in Human Colorectal Cancer Cells (SW480) by Inducing G0/G1-Phase Cell Cycle Arrest and Apoptosis |
title_short |
Jianpi Huayu Decoction Inhibits Proliferation in Human Colorectal Cancer Cells (SW480) by Inducing G0/G1-Phase Cell Cycle Arrest and Apoptosis |
title_full |
Jianpi Huayu Decoction Inhibits Proliferation in Human Colorectal Cancer Cells (SW480) by Inducing G0/G1-Phase Cell Cycle Arrest and Apoptosis |
title_fullStr |
Jianpi Huayu Decoction Inhibits Proliferation in Human Colorectal Cancer Cells (SW480) by Inducing G0/G1-Phase Cell Cycle Arrest and Apoptosis |
title_full_unstemmed |
Jianpi Huayu Decoction Inhibits Proliferation in Human Colorectal Cancer Cells (SW480) by Inducing G0/G1-Phase Cell Cycle Arrest and Apoptosis |
title_sort |
jianpi huayu decoction inhibits proliferation in human colorectal cancer cells (sw480) by inducing g0/g1-phase cell cycle arrest and apoptosis |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-427X 1741-4288 |
publishDate |
2015-01-01 |
description |
Jianpi Huayu Decoction (JHD), a Chinese medicine formula, is a typical prescription against multiple tumors in the clinical treatment, which can raise quality of life and decrease complications. The aim of this study is to assess the efficacy of JHD against human colorectal carcinoma cells (SW480) and explore its mechanism. MTT assay showed that JHD decreased the cellular viability of SW480 cells in dose-dependent and time-dependent manner. Flow cytometry analysis revealed that JHD induced G0/G1-phase cell cycle arrest in SW480 cells and had a strong apoptosis-inducing effect on SW480 cells. Meanwhile it enhanced the expression of p27, cleaved PARP, cleaved caspase-3, and Bax and decreased the levels of PARP, caspase-3, Bcl-2, CDK2, CDK4, CDK6, cyclin D1, cyclin D2, cyclin D3, and cyclin E1, which was evidenced by RT-qPCR and Western blot analysis. In conclusion, these results indicated that JHD inhibited proliferation in SW480 cells by inducing G0/G1-phase cell cycle arrest and apoptosis, providing a practicaltherapeutic strategy against colorectal cancer. |
url |
http://dx.doi.org/10.1155/2015/236506 |
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