Production of intravenous human dengue immunoglobulin from Brazilian-blood donors

• Main Authors: Frederico Leite Gouveia, Renata Mendes Batista de Oliveira, Antônio Edson de Souza Lucena, Marli Tenório Cordeiro, Ana Cristina Lima Leite
• Format: Article
• Language: English
• Published: Universidade de São Paulo 2013-12-01
• Series: Brazilian Journal of Pharmaceutical Sciences
• Subjects: Infecções virais; Dengue; Imunoglobulinas específicas; Fracionamento de plasma; Neutralização viral
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000400022&lng=en&tlng=en

Dengue represents an important health problem in Brazil and therefore there is a great need to develop a vaccine or treatment. The neutralization of the dengue virus by a specific antibody can potentially be applied to therapy. The present paper describes, for the first time, the preparation of Immunoglobulin specific for the dengue virus (anti-DENV IgG), collected from screened Brazilian blood-donations. Production was performed using the classic Cohn-Oncley process with minor modifications. The anti-DENV IgG was biochemically and biophysically characterized and fulfilled the requirements defined by the European Pharmacopoeia. The finished product was able to neutralize different virus serotypes (DENV-1, DENV-2, and DENV-3), while a commercial IgG collected from American blood donations was found to have low anti-dengue antibody titers. Overall, this anti-DENV IgG represents an important step in the study of the therapeutic potential and safety of a specific antibody that neutralizes the dengue virus in humans.