Adenosine monophosphate forms ordered arrays in multilamellar lipid matrices: insights into assembly of nucleic acid for primitive life.
A fundamental question of biology is how nucleic acids first assembled and then were incorporated into the earliest forms of cellular life 4 billion years ago. The polymerization of nucleotides is a condensation reaction in which phosphodiester bonds are formed. This reaction cannot occur in aqueous...
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doaj-b2f92c8091464a1ca45401852d10994d2020-11-25T02:12:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0185e6281010.1371/journal.pone.0062810Adenosine monophosphate forms ordered arrays in multilamellar lipid matrices: insights into assembly of nucleic acid for primitive life.Laura ToppoziniHannah DiesDavid W DeamerMaikel C RheinstädterA fundamental question of biology is how nucleic acids first assembled and then were incorporated into the earliest forms of cellular life 4 billion years ago. The polymerization of nucleotides is a condensation reaction in which phosphodiester bonds are formed. This reaction cannot occur in aqueous solutions, but guided polymerization in an anhydrous lipid environment could promote a non-enzymatic condensation reaction in which oligomers of single stranded nucleic acids are synthesized. We used X-ray scattering to investigate 5'-adenosine monophosphate (AMP) molecules captured in a multilamellar phospholipid matrix composed of dimyristoylphosphatidylcholine. Bragg peaks corresponding to the lateral organization of the confined AMP molecules were observed. Instead of forming a random array, the AMP molecules are highly entangled, with the phosphate and ribose groups in close proximity. This structure may facilitate polymerization of the nucleotides into RNA-like polymers.http://europepmc.org/articles/PMC3646914?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Laura Toppozini Hannah Dies David W Deamer Maikel C Rheinstädter |
spellingShingle |
Laura Toppozini Hannah Dies David W Deamer Maikel C Rheinstädter Adenosine monophosphate forms ordered arrays in multilamellar lipid matrices: insights into assembly of nucleic acid for primitive life. PLoS ONE |
author_facet |
Laura Toppozini Hannah Dies David W Deamer Maikel C Rheinstädter |
author_sort |
Laura Toppozini |
title |
Adenosine monophosphate forms ordered arrays in multilamellar lipid matrices: insights into assembly of nucleic acid for primitive life. |
title_short |
Adenosine monophosphate forms ordered arrays in multilamellar lipid matrices: insights into assembly of nucleic acid for primitive life. |
title_full |
Adenosine monophosphate forms ordered arrays in multilamellar lipid matrices: insights into assembly of nucleic acid for primitive life. |
title_fullStr |
Adenosine monophosphate forms ordered arrays in multilamellar lipid matrices: insights into assembly of nucleic acid for primitive life. |
title_full_unstemmed |
Adenosine monophosphate forms ordered arrays in multilamellar lipid matrices: insights into assembly of nucleic acid for primitive life. |
title_sort |
adenosine monophosphate forms ordered arrays in multilamellar lipid matrices: insights into assembly of nucleic acid for primitive life. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
A fundamental question of biology is how nucleic acids first assembled and then were incorporated into the earliest forms of cellular life 4 billion years ago. The polymerization of nucleotides is a condensation reaction in which phosphodiester bonds are formed. This reaction cannot occur in aqueous solutions, but guided polymerization in an anhydrous lipid environment could promote a non-enzymatic condensation reaction in which oligomers of single stranded nucleic acids are synthesized. We used X-ray scattering to investigate 5'-adenosine monophosphate (AMP) molecules captured in a multilamellar phospholipid matrix composed of dimyristoylphosphatidylcholine. Bragg peaks corresponding to the lateral organization of the confined AMP molecules were observed. Instead of forming a random array, the AMP molecules are highly entangled, with the phosphate and ribose groups in close proximity. This structure may facilitate polymerization of the nucleotides into RNA-like polymers. |
url |
http://europepmc.org/articles/PMC3646914?pdf=render |
work_keys_str_mv |
AT lauratoppozini adenosinemonophosphateformsorderedarraysinmultilamellarlipidmatricesinsightsintoassemblyofnucleicacidforprimitivelife AT hannahdies adenosinemonophosphateformsorderedarraysinmultilamellarlipidmatricesinsightsintoassemblyofnucleicacidforprimitivelife AT davidwdeamer adenosinemonophosphateformsorderedarraysinmultilamellarlipidmatricesinsightsintoassemblyofnucleicacidforprimitivelife AT maikelcrheinstadter adenosinemonophosphateformsorderedarraysinmultilamellarlipidmatricesinsightsintoassemblyofnucleicacidforprimitivelife |
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