Clostridium difficile PCR Ribotypes in Calves, Canada
We investigated Clostridium difficile in calves and the similarity between bovine and human C. difficile PCR ribotypes by conducting a case-control study of calves from 102 dairy farms in Canada. Fecal samples from 144 calves with diarrhea and 134 control calves were cultured for C. difficile and te...
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2006-11-01
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doaj-b2fdb29b857944c6a068f6f34ecad0ca2020-11-24T23:08:02ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592006-11-0112111730173610.3201/eid1211.051581Clostridium difficile PCR Ribotypes in Calves, CanadaAlexander Rodriguez-PalaciosHenry R. StämpfliTodd DuffieldAndrew S. PeregrineLise A. Trotz-WilliamsLuis G. ArroyoJon S. BrazierJ. Scott WeeseWe investigated Clostridium difficile in calves and the similarity between bovine and human C. difficile PCR ribotypes by conducting a case-control study of calves from 102 dairy farms in Canada. Fecal samples from 144 calves with diarrhea and 134 control calves were cultured for C. difficile and tested with an ELISA for C. difficile toxins A and B. C. difficile was isolated from 31 of 278 calves: 11 (7.6%) of 144 with diarrhea and 20 (14.9%) of 134 controls (p = 0.009). Toxins were detected in calf feces from 58 (56.8%) of 102 farms, 57 (39.6%) of 144 calves with diarrhea, and 28 (20.9%) of 134 controls (p = 0.0002). PCR ribotyping of 31 isolates showed 8 distinct patterns; 7 have been identified in humans, 2 of which have been associated with outbreaks of severe disease (PCR types 017 and 027). C. difficile may be associated with calf diarrhea, and cattle may be reservoirs of C. difficile for humans.https://wwwnc.cdc.gov/eid/article/12/11/05-1581_articleCalf diarrheaClostridium difficilehumansCryptosporidiumcattlefluoroquinolones |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alexander Rodriguez-Palacios Henry R. Stämpfli Todd Duffield Andrew S. Peregrine Lise A. Trotz-Williams Luis G. Arroyo Jon S. Brazier J. Scott Weese |
spellingShingle |
Alexander Rodriguez-Palacios Henry R. Stämpfli Todd Duffield Andrew S. Peregrine Lise A. Trotz-Williams Luis G. Arroyo Jon S. Brazier J. Scott Weese Clostridium difficile PCR Ribotypes in Calves, Canada Emerging Infectious Diseases Calf diarrhea Clostridium difficile humans Cryptosporidium cattle fluoroquinolones |
author_facet |
Alexander Rodriguez-Palacios Henry R. Stämpfli Todd Duffield Andrew S. Peregrine Lise A. Trotz-Williams Luis G. Arroyo Jon S. Brazier J. Scott Weese |
author_sort |
Alexander Rodriguez-Palacios |
title |
Clostridium difficile PCR Ribotypes in Calves, Canada |
title_short |
Clostridium difficile PCR Ribotypes in Calves, Canada |
title_full |
Clostridium difficile PCR Ribotypes in Calves, Canada |
title_fullStr |
Clostridium difficile PCR Ribotypes in Calves, Canada |
title_full_unstemmed |
Clostridium difficile PCR Ribotypes in Calves, Canada |
title_sort |
clostridium difficile pcr ribotypes in calves, canada |
publisher |
Centers for Disease Control and Prevention |
series |
Emerging Infectious Diseases |
issn |
1080-6040 1080-6059 |
publishDate |
2006-11-01 |
description |
We investigated Clostridium difficile in calves and the similarity between bovine and human C. difficile PCR ribotypes by conducting a case-control study of calves from 102 dairy farms in Canada. Fecal samples from 144 calves with diarrhea and 134 control calves were cultured for C. difficile and tested with an ELISA for C. difficile toxins A and B. C. difficile was isolated from 31 of 278 calves: 11 (7.6%) of 144 with diarrhea and 20 (14.9%) of 134 controls (p = 0.009). Toxins were detected in calf feces from 58 (56.8%) of 102 farms, 57 (39.6%) of 144 calves with diarrhea, and 28 (20.9%) of 134 controls (p = 0.0002). PCR ribotyping of 31 isolates showed 8 distinct patterns; 7 have been identified in humans, 2 of which have been associated with outbreaks of severe disease (PCR types 017 and 027). C. difficile may be associated with calf diarrhea, and cattle may be reservoirs of C. difficile for humans. |
topic |
Calf diarrhea Clostridium difficile humans Cryptosporidium cattle fluoroquinolones |
url |
https://wwwnc.cdc.gov/eid/article/12/11/05-1581_article |
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