Simultaneous downregulation of miR-21 and upregulation of miR-7 has anti-tumor efficacy
Abstract Dysregulation of miRNA expression has been implicated in cancer. Numerous strategies have been explored to modulate miR but sub-optimal delivery and inability to concurrently target multiple pathways involved in tumor progression have limited their efficacy. In this study, we explored the p...
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doaj-b320c564c7ac41faa451f7f557aa22c32021-02-07T12:43:52ZengNature Publishing GroupScientific Reports2045-23222020-02-0110111010.1038/s41598-020-58072-wSimultaneous downregulation of miR-21 and upregulation of miR-7 has anti-tumor efficacyDeepak Bhere0Nahid Arghiani1Esther Revai Lechtich2Yizheng Yao3Sarah Alsaab4Fengfeng Bei5Maryam M. Matin6Khalid Shah7Center for Stem Cell Therapeutics and Imaging (CSTI), Brigham and Women’s Hospital, Harvard Medical SchoolCenter for Stem Cell Therapeutics and Imaging (CSTI), Brigham and Women’s Hospital, Harvard Medical SchoolCenter for Stem Cell Therapeutics and Imaging (CSTI), Brigham and Women’s Hospital, Harvard Medical SchoolDepartment of Neurosurgery, Brigham and Women’s Hospital, Harvard Medical SchoolCenter for Stem Cell Therapeutics and Imaging (CSTI), Brigham and Women’s Hospital, Harvard Medical SchoolDepartment of Neurosurgery, Brigham and Women’s Hospital, Harvard Medical SchoolDepartment of Biology and Institute of Biotechnology, Ferdowsi University of MashhadCenter for Stem Cell Therapeutics and Imaging (CSTI), Brigham and Women’s Hospital, Harvard Medical SchoolAbstract Dysregulation of miRNA expression has been implicated in cancer. Numerous strategies have been explored to modulate miR but sub-optimal delivery and inability to concurrently target multiple pathways involved in tumor progression have limited their efficacy. In this study, we explored the potential co-modulation of upregulated miR-21 and downregulated miR-7 to enhance therapeutic outcomes in heterogenic tumor types. We first engineered lentiviral (LV) and adeno-associated viral (AAV) vectors that preferentially express anti-sense miR against miR-21(miRzip-21) and show that modulating miR-21 via miRzip extensively targets tumor cell proliferation, migration and invasion in vitro in a broad spectrum of cancer types and has therapeutic efficacy in vivo. Next, we show a significantly increased expression of caspase-mediated apoptosis by simultaneously downregulating miR-21 and upregulating miR-7 in different tumor cells. In vivo co-treatment with AAV-miRzip-21 and AAV-miR-7 in mice bearing malignant brain tumors resulted in significantly decreased tumor burden with a corresponding increase in survival. To our knowledge, this is the first study that demonstrates the therapeutic efficacy of simultaneously upregulating miR-7 and downregulating miR-21 and establishes a roadmap towards clinical translation of modulating miRs for various cancer types.https://doi.org/10.1038/s41598-020-58072-w |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Deepak Bhere Nahid Arghiani Esther Revai Lechtich Yizheng Yao Sarah Alsaab Fengfeng Bei Maryam M. Matin Khalid Shah |
spellingShingle |
Deepak Bhere Nahid Arghiani Esther Revai Lechtich Yizheng Yao Sarah Alsaab Fengfeng Bei Maryam M. Matin Khalid Shah Simultaneous downregulation of miR-21 and upregulation of miR-7 has anti-tumor efficacy Scientific Reports |
author_facet |
Deepak Bhere Nahid Arghiani Esther Revai Lechtich Yizheng Yao Sarah Alsaab Fengfeng Bei Maryam M. Matin Khalid Shah |
author_sort |
Deepak Bhere |
title |
Simultaneous downregulation of miR-21 and upregulation of miR-7 has anti-tumor efficacy |
title_short |
Simultaneous downregulation of miR-21 and upregulation of miR-7 has anti-tumor efficacy |
title_full |
Simultaneous downregulation of miR-21 and upregulation of miR-7 has anti-tumor efficacy |
title_fullStr |
Simultaneous downregulation of miR-21 and upregulation of miR-7 has anti-tumor efficacy |
title_full_unstemmed |
Simultaneous downregulation of miR-21 and upregulation of miR-7 has anti-tumor efficacy |
title_sort |
simultaneous downregulation of mir-21 and upregulation of mir-7 has anti-tumor efficacy |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2020-02-01 |
description |
Abstract Dysregulation of miRNA expression has been implicated in cancer. Numerous strategies have been explored to modulate miR but sub-optimal delivery and inability to concurrently target multiple pathways involved in tumor progression have limited their efficacy. In this study, we explored the potential co-modulation of upregulated miR-21 and downregulated miR-7 to enhance therapeutic outcomes in heterogenic tumor types. We first engineered lentiviral (LV) and adeno-associated viral (AAV) vectors that preferentially express anti-sense miR against miR-21(miRzip-21) and show that modulating miR-21 via miRzip extensively targets tumor cell proliferation, migration and invasion in vitro in a broad spectrum of cancer types and has therapeutic efficacy in vivo. Next, we show a significantly increased expression of caspase-mediated apoptosis by simultaneously downregulating miR-21 and upregulating miR-7 in different tumor cells. In vivo co-treatment with AAV-miRzip-21 and AAV-miR-7 in mice bearing malignant brain tumors resulted in significantly decreased tumor burden with a corresponding increase in survival. To our knowledge, this is the first study that demonstrates the therapeutic efficacy of simultaneously upregulating miR-7 and downregulating miR-21 and establishes a roadmap towards clinical translation of modulating miRs for various cancer types. |
url |
https://doi.org/10.1038/s41598-020-58072-w |
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