Novel protein extraction approach using micro-sized chamber for evaluation of proteins eluted from formalin-fixed paraffin-embedded tissue sections
<p>Abstract</p> <p>We describe a novel antigen-retrieval method using a micro-sized chamber for mass spectrometry (MS) analysis to identify proteins that are preferentially eluted from formalin-fixed paraffin-embedded (FFPE) samples. This approach revealed that heat-induced antigen...
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doaj-b336eef5d9c048c88e72e96f285772602020-11-25T01:56:13ZengBMCProteome Science1477-59562012-03-011011910.1186/1477-5956-10-19Novel protein extraction approach using micro-sized chamber for evaluation of proteins eluted from formalin-fixed paraffin-embedded tissue sectionsHatakeyama KeiichiWakabayashi-Nakao KanakoAoki YutakaOgura Shun-ichiroYamaguchi KenNakajima TakashiSato Taka-AkiMochizuki TohruHayashi Isamu<p>Abstract</p> <p>We describe a novel antigen-retrieval method using a micro-sized chamber for mass spectrometry (MS) analysis to identify proteins that are preferentially eluted from formalin-fixed paraffin-embedded (FFPE) samples. This approach revealed that heat-induced antigen retrieval (HIAR) from an FFPE sample fixed on a glass slide not only improves protein identification, but also facilitates preferential elution of protein subsets corresponding to the properties of antigen-retrieval buffers. Our approach may contribute to an understanding of the mechanism of HIAR.</p> http://www.proteomesci.com/content/10/1/19Antigen retrievalColon adenomaColon cancerFFPE tissueGastric cancerHeat-induced antigen retrievalMass spectrometryMicro-sized chamber |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hatakeyama Keiichi Wakabayashi-Nakao Kanako Aoki Yutaka Ogura Shun-ichiro Yamaguchi Ken Nakajima Takashi Sato Taka-Aki Mochizuki Tohru Hayashi Isamu |
spellingShingle |
Hatakeyama Keiichi Wakabayashi-Nakao Kanako Aoki Yutaka Ogura Shun-ichiro Yamaguchi Ken Nakajima Takashi Sato Taka-Aki Mochizuki Tohru Hayashi Isamu Novel protein extraction approach using micro-sized chamber for evaluation of proteins eluted from formalin-fixed paraffin-embedded tissue sections Proteome Science Antigen retrieval Colon adenoma Colon cancer FFPE tissue Gastric cancer Heat-induced antigen retrieval Mass spectrometry Micro-sized chamber |
author_facet |
Hatakeyama Keiichi Wakabayashi-Nakao Kanako Aoki Yutaka Ogura Shun-ichiro Yamaguchi Ken Nakajima Takashi Sato Taka-Aki Mochizuki Tohru Hayashi Isamu |
author_sort |
Hatakeyama Keiichi |
title |
Novel protein extraction approach using micro-sized chamber for evaluation of proteins eluted from formalin-fixed paraffin-embedded tissue sections |
title_short |
Novel protein extraction approach using micro-sized chamber for evaluation of proteins eluted from formalin-fixed paraffin-embedded tissue sections |
title_full |
Novel protein extraction approach using micro-sized chamber for evaluation of proteins eluted from formalin-fixed paraffin-embedded tissue sections |
title_fullStr |
Novel protein extraction approach using micro-sized chamber for evaluation of proteins eluted from formalin-fixed paraffin-embedded tissue sections |
title_full_unstemmed |
Novel protein extraction approach using micro-sized chamber for evaluation of proteins eluted from formalin-fixed paraffin-embedded tissue sections |
title_sort |
novel protein extraction approach using micro-sized chamber for evaluation of proteins eluted from formalin-fixed paraffin-embedded tissue sections |
publisher |
BMC |
series |
Proteome Science |
issn |
1477-5956 |
publishDate |
2012-03-01 |
description |
<p>Abstract</p> <p>We describe a novel antigen-retrieval method using a micro-sized chamber for mass spectrometry (MS) analysis to identify proteins that are preferentially eluted from formalin-fixed paraffin-embedded (FFPE) samples. This approach revealed that heat-induced antigen retrieval (HIAR) from an FFPE sample fixed on a glass slide not only improves protein identification, but also facilitates preferential elution of protein subsets corresponding to the properties of antigen-retrieval buffers. Our approach may contribute to an understanding of the mechanism of HIAR.</p> |
topic |
Antigen retrieval Colon adenoma Colon cancer FFPE tissue Gastric cancer Heat-induced antigen retrieval Mass spectrometry Micro-sized chamber |
url |
http://www.proteomesci.com/content/10/1/19 |
work_keys_str_mv |
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