Novel protein extraction approach using micro-sized chamber for evaluation of proteins eluted from formalin-fixed paraffin-embedded tissue sections

• Main Authors: Hatakeyama Keiichi, Wakabayashi-Nakao Kanako, Aoki Yutaka, Ogura Shun-ichiro, Yamaguchi Ken, Nakajima Takashi, Sato Taka-Aki, Mochizuki Tohru, Hayashi Isamu
• Format: Article
• Language: English
• Published: BMC 2012-03-01
• Series: Proteome Science
• Subjects: Antigen retrieval; Colon adenoma; Colon cancer; FFPE tissue; Gastric cancer; Heat-induced antigen retrieval; Mass spectrometry; Micro-sized chamber
• Online Access: http://www.proteomesci.com/content/10/1/19

<p>Abstract</p> <p>We describe a novel antigen-retrieval method using a micro-sized chamber for mass spectrometry (MS) analysis to identify proteins that are preferentially eluted from formalin-fixed paraffin-embedded (FFPE) samples. This approach revealed that heat-induced antigen retrieval (HIAR) from an FFPE sample fixed on a glass slide not only improves protein identification, but also facilitates preferential elution of protein subsets corresponding to the properties of antigen-retrieval buffers. Our approach may contribute to an understanding of the mechanism of HIAR.</p>