Impact of Albendazole on Cytokine and Chemokine Response Profiles in Echinococcus multilocularis-Inoculated Mice
Objective. Alveolar echinococcosis (AE) is a zoonosis caused by the larval stage of the metacestode Echinococcosis multilocularis with a tumor-like behavior in the targeted organ, especially in the liver. Surgery with albendazole is first-line modality for AE. Drug discontinuation is usually based u...
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doaj-b35f12b9aa414360a79ee4d9591165782021-05-17T00:00:25ZengHindawi LimitedBioMed Research International2314-61412021-01-01202110.1155/2021/6628814Impact of Albendazole on Cytokine and Chemokine Response Profiles in Echinococcus multilocularis-Inoculated MiceJing Wu0Hai-zhang Ma1Shadike Apaer2Nuerzatijiang Anweier3Qi Zeng4Xiafukati Fulati5Tao Li6Jin-ming Zhao7Hao Wen8Tuerhongjiang Tuxun9State Key Laboratory of PathogenesisDepartment of Organ TransplantState Key Laboratory of PathogenesisDepartment of Liver & Laparoscopic SurgeryDepartment of Liver & Laparoscopic SurgeryDepartment of Liver & Laparoscopic SurgeryDepartment of Liver & Laparoscopic SurgeryDepartment of Liver & Laparoscopic SurgeryState Key Laboratory of PathogenesisState Key Laboratory of PathogenesisObjective. Alveolar echinococcosis (AE) is a zoonosis caused by the larval stage of the metacestode Echinococcosis multilocularis with a tumor-like behavior in the targeted organ, especially in the liver. Surgery with albendazole is first-line modality for AE. Drug discontinuation is usually based upon the parasitic viability shown by the positron emission tomography (PET) scan. However, as a demanding and expensive method, it is not widely practiced in majority of the endemic regions. Further understanding on the cytokine and chemokine response profiles in AE patients may provide an interesting insight for potential markers in viability assessment. Methods. Mice were inoculated with Echinococcus multilocularis intrahepatically to develop the hepatic AE murine model. Oral albendazole administration was then applied for three months after the first inoculation, and peripheral and regional immune cells including type 1 T helper cells (Th), Th2, Th17, regulatory T (Treg) cells, related cytokines, and chemokines were examined. Results. The hepatic AE lesion was confirmed by ultrasound examination resulting in a successful rate of 70%. Among the 17 cytokines and chemokines detected, plasma levels of IL-23 were significantly higher in E. multilocularis-infected mice when compared to the control group; furthermore, more obvious increasing levels were found after albendazole treatment (p<0.05). All chemokine levels other than eotaxin and MCP-3 were slightly higher in E. multilocularis-infected mice compared to the control group (p>0.05). Eotaxin levels were significantly decreased in mice with E. multilocularis infection followed by albendazole treatment (p<0.05). Both IL-17A and IL-23 expressions in hepatic AE lesions were significantly higher and related with disease activity. Conclusion. Albendazole administration influenced the balance of immune response and promotes the secretion of proinflammatory factors which is beneficial to parasite clearance. IL-23 seems to be associated with the successful albendazole treatment in mice with E. multilocularis infection; such a change could be translated into clinical application in the near future.http://dx.doi.org/10.1155/2021/6628814 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jing Wu Hai-zhang Ma Shadike Apaer Nuerzatijiang Anweier Qi Zeng Xiafukati Fulati Tao Li Jin-ming Zhao Hao Wen Tuerhongjiang Tuxun |
spellingShingle |
Jing Wu Hai-zhang Ma Shadike Apaer Nuerzatijiang Anweier Qi Zeng Xiafukati Fulati Tao Li Jin-ming Zhao Hao Wen Tuerhongjiang Tuxun Impact of Albendazole on Cytokine and Chemokine Response Profiles in Echinococcus multilocularis-Inoculated Mice BioMed Research International |
author_facet |
Jing Wu Hai-zhang Ma Shadike Apaer Nuerzatijiang Anweier Qi Zeng Xiafukati Fulati Tao Li Jin-ming Zhao Hao Wen Tuerhongjiang Tuxun |
author_sort |
Jing Wu |
title |
Impact of Albendazole on Cytokine and Chemokine Response Profiles in Echinococcus multilocularis-Inoculated Mice |
title_short |
Impact of Albendazole on Cytokine and Chemokine Response Profiles in Echinococcus multilocularis-Inoculated Mice |
title_full |
Impact of Albendazole on Cytokine and Chemokine Response Profiles in Echinococcus multilocularis-Inoculated Mice |
title_fullStr |
Impact of Albendazole on Cytokine and Chemokine Response Profiles in Echinococcus multilocularis-Inoculated Mice |
title_full_unstemmed |
Impact of Albendazole on Cytokine and Chemokine Response Profiles in Echinococcus multilocularis-Inoculated Mice |
title_sort |
impact of albendazole on cytokine and chemokine response profiles in echinococcus multilocularis-inoculated mice |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6141 |
publishDate |
2021-01-01 |
description |
Objective. Alveolar echinococcosis (AE) is a zoonosis caused by the larval stage of the metacestode Echinococcosis multilocularis with a tumor-like behavior in the targeted organ, especially in the liver. Surgery with albendazole is first-line modality for AE. Drug discontinuation is usually based upon the parasitic viability shown by the positron emission tomography (PET) scan. However, as a demanding and expensive method, it is not widely practiced in majority of the endemic regions. Further understanding on the cytokine and chemokine response profiles in AE patients may provide an interesting insight for potential markers in viability assessment. Methods. Mice were inoculated with Echinococcus multilocularis intrahepatically to develop the hepatic AE murine model. Oral albendazole administration was then applied for three months after the first inoculation, and peripheral and regional immune cells including type 1 T helper cells (Th), Th2, Th17, regulatory T (Treg) cells, related cytokines, and chemokines were examined. Results. The hepatic AE lesion was confirmed by ultrasound examination resulting in a successful rate of 70%. Among the 17 cytokines and chemokines detected, plasma levels of IL-23 were significantly higher in E. multilocularis-infected mice when compared to the control group; furthermore, more obvious increasing levels were found after albendazole treatment (p<0.05). All chemokine levels other than eotaxin and MCP-3 were slightly higher in E. multilocularis-infected mice compared to the control group (p>0.05). Eotaxin levels were significantly decreased in mice with E. multilocularis infection followed by albendazole treatment (p<0.05). Both IL-17A and IL-23 expressions in hepatic AE lesions were significantly higher and related with disease activity. Conclusion. Albendazole administration influenced the balance of immune response and promotes the secretion of proinflammatory factors which is beneficial to parasite clearance. IL-23 seems to be associated with the successful albendazole treatment in mice with E. multilocularis infection; such a change could be translated into clinical application in the near future. |
url |
http://dx.doi.org/10.1155/2021/6628814 |
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