FAM225A facilitates colorectal cancer progression by sponging miR‐613 to regulate NOTCH3

• Main Authors: Xuexiu Zhang, Haoling Shi, Jianning Yao, Yanle Li, Bing Gao, Yanzhen Zhang, Chunfeng Wang, Haining Zhou, Lianfeng Zhang
• Format: Article
• Language: English
• Published: Wiley 2020-06-01
• Series: Cancer Medicine
• Subjects: colorectal cancer; FAM225A; miR‐613; NOTCH3
• Online Access: https://doi.org/10.1002/cam4.3053

Abstract Colorectal cancer (CRC) is a fatal disease ranking the third among the commonplace cancer types around the world. It is extremely significant to exploit effective treatments against CRC. FAM225A was proved to influence cell progression and forecast unfavorable prognosis in nasopharyngeal carcinoma. The role and function mechanism of FAM225A are still unclear in CRC. In this research, FAM225A was discovered presenting much higher expression in CRC tissues and cell lines. In addition, depleting FAM225A was capable of inhibiting cell proliferation, migration, and epithelial‐to‐mesenchymal transition (EMT) progress, and enhancing cell apoptosis ability. Furthermore, miR‐613 exerted important effects as a mediator between FAM225A and NOTCH3. NOTCH3 was negatively correlated with miR‐613, whereas was positively associated with FAM225A. Via competitively binding with miR‐613, FAM225A positively regulated NOTCH3 expression. FAM225A facilitated CRC occurrence and development through positively regulating NOTCH3 expression by binding with miR‐613. In a word, FAM225A/miR‐613/NOTCH3 axis may play a tumor‐facilitator in CRC cell progression. These data manifested the pivotal effect of FAM225A/miR‐613/NOTCH3 pathway in CRC cell proliferation, apoptosis, and migration process. The findings may provide some theoretical basis and different perspective for CRC treatment.