An Exo-Polygalacturonase Pgc4 Regulates Aerial Hyphal Growth and Virulence in <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> race 4
<i>Fusarium oxysporum</i> f. sp. <i>cubense</i> race 4 (Foc4) causes Fusarium wilt that affects banana plants, and hence, the molecular mechanisms of its virulence need to be investigated. We purified an exo-polygalacturonase (exo-PG), Pgc4, from Foc4. Pgc4 has an apparent mo...
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doaj-b39f27a8914b4dcbb2f34422ca57e9e62020-11-25T03:20:16ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-08-01215886588610.3390/ijms21165886An Exo-Polygalacturonase Pgc4 Regulates Aerial Hyphal Growth and Virulence in <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> race 4Zhangyong Dong0Mei Luo1Zhenzhong Wang2Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, ChinaInnovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, ChinaLaboratory of Physiological Plant Pathology, South China Agricultural University, Guangzhou 510642, China<i>Fusarium oxysporum</i> f. sp. <i>cubense</i> race 4 (Foc4) causes Fusarium wilt that affects banana plants, and hence, the molecular mechanisms of its virulence need to be investigated. We purified an exo-polygalacturonase (exo-PG), Pgc4, from Foc4. Pgc4 has an apparent molecular weight of 50.87 kDa based on sodium dodecyl sulphate–polyacrylamide gel electrophoresis. We further performed its sequence analysis and biochemical characterization. The two <i>pgc4</i> genes encoding Pgc4 from Foc4 and Foc1 were 1434 bp in length and encoded 477 amino acids with differences, due to some nucleotide differences between the two. The <i>K</i>m and <i>V</i>max values of Pgc4 purified from Foc4 were determined to be 0.45 mg/mL and 105.26 Units·mg·protein<sup>−1</sup> ·min<sup>−1</sup>, respectively. The recombinant proteins, r-Foc1-Pgc4 and r-Foc4-Pgc4, were expressed and purified from <i>Pichia pastoris</i> and showed optimal Pgc4 activity at 55 °C and pH 4.0; both could induce tissue maceration and necrosis in the “Guangfen-1” and “Baxi” varieties of banana but to a different extent. Phenotypic assays and complementation analyses revealed that, compared to the wild-type, the generated Foc4Δ<i>pgc4</i> mutant strain showed a lower aerial hyphal growth, grew slower, and had a reduced virulence. Therefore, our results demonstrate the function of Pgc4 as a pathogenicity factor of Foc4.https://www.mdpi.com/1422-0067/21/16/5886polygalacturonasevirulence<i>Fusarium oxysporum</i> f. sp. <i>cubense</i> race 4 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhangyong Dong Mei Luo Zhenzhong Wang |
spellingShingle |
Zhangyong Dong Mei Luo Zhenzhong Wang An Exo-Polygalacturonase Pgc4 Regulates Aerial Hyphal Growth and Virulence in <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> race 4 International Journal of Molecular Sciences polygalacturonase virulence <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> race 4 |
author_facet |
Zhangyong Dong Mei Luo Zhenzhong Wang |
author_sort |
Zhangyong Dong |
title |
An Exo-Polygalacturonase Pgc4 Regulates Aerial Hyphal Growth and Virulence in <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> race 4 |
title_short |
An Exo-Polygalacturonase Pgc4 Regulates Aerial Hyphal Growth and Virulence in <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> race 4 |
title_full |
An Exo-Polygalacturonase Pgc4 Regulates Aerial Hyphal Growth and Virulence in <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> race 4 |
title_fullStr |
An Exo-Polygalacturonase Pgc4 Regulates Aerial Hyphal Growth and Virulence in <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> race 4 |
title_full_unstemmed |
An Exo-Polygalacturonase Pgc4 Regulates Aerial Hyphal Growth and Virulence in <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> race 4 |
title_sort |
exo-polygalacturonase pgc4 regulates aerial hyphal growth and virulence in <i>fusarium oxysporum</i> f. sp. <i>cubense</i> race 4 |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2020-08-01 |
description |
<i>Fusarium oxysporum</i> f. sp. <i>cubense</i> race 4 (Foc4) causes Fusarium wilt that affects banana plants, and hence, the molecular mechanisms of its virulence need to be investigated. We purified an exo-polygalacturonase (exo-PG), Pgc4, from Foc4. Pgc4 has an apparent molecular weight of 50.87 kDa based on sodium dodecyl sulphate–polyacrylamide gel electrophoresis. We further performed its sequence analysis and biochemical characterization. The two <i>pgc4</i> genes encoding Pgc4 from Foc4 and Foc1 were 1434 bp in length and encoded 477 amino acids with differences, due to some nucleotide differences between the two. The <i>K</i>m and <i>V</i>max values of Pgc4 purified from Foc4 were determined to be 0.45 mg/mL and 105.26 Units·mg·protein<sup>−1</sup> ·min<sup>−1</sup>, respectively. The recombinant proteins, r-Foc1-Pgc4 and r-Foc4-Pgc4, were expressed and purified from <i>Pichia pastoris</i> and showed optimal Pgc4 activity at 55 °C and pH 4.0; both could induce tissue maceration and necrosis in the “Guangfen-1” and “Baxi” varieties of banana but to a different extent. Phenotypic assays and complementation analyses revealed that, compared to the wild-type, the generated Foc4Δ<i>pgc4</i> mutant strain showed a lower aerial hyphal growth, grew slower, and had a reduced virulence. Therefore, our results demonstrate the function of Pgc4 as a pathogenicity factor of Foc4. |
topic |
polygalacturonase virulence <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> race 4 |
url |
https://www.mdpi.com/1422-0067/21/16/5886 |
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